公开(公告)号：US20110046339A1

公开(公告)日：2011-02-24

申请号：US12312636

申请日：2007-11-21

申请人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

发明人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

IPC分类号： C08G63/06 ,  C12P7/62

CPC分类号： C08G63/06 ,  C12P7/625

摘要： The present invention relates to a copolymer comprising 3-hydroxyalkanoate monomer unit and lactate monomer unit, or their preparing method. More specifically, the present invention relates to a method for preparing a copolymer comprising lactate monomer and 3-hydroxyalkanoate monomer, wherein the method comprises culturing a cell or plant comprising the gene of enzyme converting lactate and 3-hydroxyalkanoate into lactyl-CoA and 3-hydroxyalkanoyl-CoA, respectively, and polyhydroxyalkanoate synthase gene together, and the copolymer made by the method. The copolymer of the present invention is a biodegradable polymer being able to be usefully used instead of conventional synthetic plastic, and the copolymer can be used also for medical use.

摘要翻译： 本发明涉及包含3-羟基链烷酸酯单体单元和乳酸单体单元的共聚物或其制备方法。 更具体地说，本发明涉及一种制备包含乳酸单体和3-羟基链烷酸酯单体的共聚物的方法，其中所述方法包括将包含转化乳酸酯和3-羟基链烷酸酯的酶的基因的细胞或植物培养成乳酰CoA和3- 羟基烷酰基-CoA和聚羟基链烷酸酯合酶基因，以及通过该方法制备的共聚物。 本发明的共聚物是可以有效地代替常规合成塑料的可生物降解的聚合物，并且共聚物也可以用于医疗用途。

公开(公告)号：US20100222545A1

公开(公告)日：2010-09-02

申请号：US12312635

申请日：2007-11-21

申请人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-Ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

发明人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-Ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

IPC分类号： C08G63/06 ,  C12P7/62

CPC分类号： C08G63/06 ,  C12N9/1025 ,  C12N15/8243 ,  C12N15/8245 ,  C12P7/625

摘要： The present invention relates to a copolymer comprising 4-hydroxybutyrate monomer unit and lactate monomer unit, a copolymer 4-hydroxybutyrate monomer unit, lactate monomer unit and 3-hydroxyalkanoate, or their preparing method. More specifically, the present invention relates to a method for preparing a copolymer comprising lactate monomer; 4-hydroxybutyrate monomer; and optionally 3-hydroxyalkanoate, wherein the method comprises culturing a cell or plant comprising the gene of enzyme converting lactate and 3-hydroxyalkanoate into lactyl-CoA and 3-hydroxyalkanoyl-CoA, respectively, phosphotransbutylase gene, butyrate kinase gene and polyhydroxyalkanoate synthase gene together, and the copolymer made by the method. The copolymer of the present invention is a biodegradable polymer being able to be usefully used instead of conventional synthetic plastic, and the copolymer can be used for medical use.

摘要翻译： 本发明涉及包含4-羟基丁酸酯单体单元和乳酸酯单体单元，共聚物4-羟基丁酸酯单体单元，乳酸酯单体单元和3-羟基链烷酸酯的共聚物或其制备方法。 更具体地说，本发明涉及制备包含乳酸酯单体的共聚物的方法; 4-羟基丁酸酯单体; 和任选的3-羟基链烷酸酯，其中所述方法包括分别将含有转化乳酸盐和3-羟基链烷酸酯的酶的基因的细胞或植物分别培养成乳酰基-CoA和3-羟基烷酰基-CoA，磷酸转移酶基因，丁酸酯激酶基因和聚羟基链烷酸酯合酶基因 和通过该方法制备的共聚物。 本发明的共聚物是能够有效地代替常规合成塑料的可生物降解聚合物，该共聚物可用于医疗用途。

公开(公告)号：US09096860B2

公开(公告)日：2015-08-04

申请号：US12676840

申请日：2008-08-13

申请人： Si-Jae Park ,  Sang-Hyun Lee ,  Sang-Yup Lee ,  Eun-Jeong Lee

发明人： Si-Jae Park ,  Sang-Hyun Lee ,  Sang-Yup Lee ,  Eun-Jeong Lee

IPC分类号： C12N1/20 ,  C12P7/18 ,  C12N15/00 ,  C07H21/04 ,  C12N15/77

CPC分类号： C12N15/77 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12P7/18 ,  C12Y101/01061 ,  C12Y102/01024 ,  C12Y103/99002

摘要： A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.

摘要翻译： 提供能够生产1,4-丁二醇的突变体和使用其制备1,4-丁二醇的方法。 通过在能够产生琥珀酸的微生物中引入和扩增编码将琥珀酸酯转化成4-羟基丁酸酯和4-羟基丁酸酯的基因转化成1,4-丁二醇来制备突变体微生物。 该方法包括在含有碳水化合物的培养基中培养该突变体并从培养物中获得1,4-丁二醇。 因此，在化学工业中必不可少的1,4-丁二醇可以在生物过程中制备。

公开(公告)号：US08383379B2

公开(公告)日：2013-02-26

申请号：US12312635

申请日：2007-11-21

申请人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-Ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

发明人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-Ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

IPC分类号： C12N9/00 ,  C07H21/04 ,  A01N63/00

CPC分类号： C08G63/06 ,  C12N9/1025 ,  C12N15/8243 ,  C12N15/8245 ,  C12P7/625

摘要： The present invention relates to a copolymer comprising 4-hydroxybutyrate monomer unit and lactate monomer unit, a copolymer 4-hydroxybutyrate monomer unit, lactate monomer unit and 3-hydroxyalkanoate, or their preparing method. More specifically, the present invention relates to a method for preparing a copolymer comprising lactate monomer; 4-hydroxybutyrate monomer; and optionally 3-hydroxyalkanoate, wherein the method comprises culturing a cell or plant comprising the gene of enzyme converting lactate and 3-hydroxyalkanoate into lactyl-CoA and 3-hydroxyalkanoyl-CoA, respectively, phosphotransbutylase gene, butyrate kinase gene and polyhydroxyalkanoate synthase gene together, and the copolymer made by the method. The copolymer of the present invention is a biodegradable polymer being able to be usefully used instead of conventional synthetic plastic, and the copolymer can be used for medical use.

摘要翻译： 本发明涉及包含4-羟基丁酸酯单体单元和乳酸酯单体单元，共聚物4-羟基丁酸酯单体单元，乳酸酯单体单元和3-羟基链烷酸酯的共聚物或其制备方法。 更具体地说，本发明涉及制备包含乳酸酯单体的共聚物的方法; 4-羟基丁酸酯单体; 和任选的3-羟基链烷酸酯，其中所述方法包括分别将含有转化乳酸盐和3-羟基链烷酸酯的酶的基因的细胞或植物分别培养成乳酰基-CoA和3-羟基烷酰基-CoA，磷酸转移酶基因，丁酸酯激酶基因和聚羟基链烷酸酯合酶基因 和通过该方法制备的共聚物。 本发明的共聚物是能够有效地代替常规合成塑料的可生物降解聚合物，该共聚物可用于医疗用途。

公开(公告)号：US08765402B2

公开(公告)日：2014-07-01

申请号：US12312636

申请日：2007-11-21

申请人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-Ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

发明人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-Ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

IPC分类号： C12P1/04

CPC分类号： C08G63/06 ,  C12P7/625

摘要： The present invention relates to a copolymer comprising 3-hydroxyalkanoate monomer unit and lactate monomer unit, or their preparing method. More specifically, the present invention relates to a method for preparing a copolymer comprising lactate monomer and 3-hydroxyalkanoate monomer, wherein the method comprises culturing a cell or plant comprising the gene of enzyme converting lactate and 3-hydroxyalkanoate into lactyl-CoA and 3-hydroxyalkanoyl-CoA, respectively, and polyhydroxyalkanoate synthase gene together, and the copolymer made by the method. The copolymer of the present invention is a biodegradable polymer being able to be usefully used instead of conventional synthetic plastic, and the copolymer can be used also for medical use.

摘要翻译： 本发明涉及包含3-羟基链烷酸酯单体单元和乳酸单体单元的共聚物或其制备方法。 更具体地说，本发明涉及一种制备包含乳酸单体和3-羟基链烷酸酯单体的共聚物的方法，其中所述方法包括将包含转化乳酸酯和3-羟基链烷酸酯的酶的基因的细胞或植物培养成乳酰CoA和3- 羟基烷酰基-CoA和聚羟基链烷酸酯合酶基因，以及通过该方法制备的共聚物。 本发明的共聚物是可以有效地代替常规合成塑料的可生物降解的聚合物，并且共聚物也可以用于医疗用途。

公开(公告)号：US08541652B2

公开(公告)日：2013-09-24

申请号：US12312676

申请日：2007-11-21

申请人： Taek-Ho Yang ,  Hye-Ok Kang ,  Si-Jae Park ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-wan Kim ,  Sang-Yup Lee

发明人： Taek-Ho Yang ,  Hye-Ok Kang ,  Si-Jae Park ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-wan Kim ,  Sang-Yup Lee

IPC分类号： C12N15/31 ,  C12N15/52 ,  C12N15/82

CPC分类号： C12N9/1025 ,  C12N15/8243 ,  C12P7/625

摘要： The present invention relates to polyhydroxyalkanoate synthase (PHA synthase) mutant originated from Pseudomonas sp. 6-19 (KCTC 11027BP) which can prepare lactate polymer and/or copolymer by using lactyl-CoA as a substrate. The present invention relates to a method for preparing lactate polymer and/or copolymer with the synthase mutant. The polyhydroxyalkanoate synthase mutants of the present invention originated from Pseudomonas sp. 6-19 can efficiently prepare lactate polymer and/or copolymer by using as a substrate lactyl-CoA which is difficult to be used as a substrate by conventional polyhydroxyalkanoate synthase.

摘要翻译： 本发明涉及源于假单胞菌属的聚羟基链烷酸酯合成酶（PHA合酶）突变体。 6-19（KCTC 11027BP），其可以通过使用乳酰辅酶A作为底物制备乳酸聚合物和/或共聚物。 本发明涉及一种用合成酶突变体制备乳酸聚合物和/或共聚物的方法。 本发明的聚羟基链烷酸酯合酶突变体起源于假单胞菌属 6-19可以通过使用难以用作常规聚羟基链烷酸酯合成酶的底物的底物乳酰基辅酶A来有效地制备乳酸聚合物和/或共聚物。

公开(公告)号：US20100050298A1

公开(公告)日：2010-02-25

申请号：US12312676

申请日：2007-11-21

申请人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-Ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

发明人： Si-Jae Park ,  Taek-Ho Yang ,  Hye-Ok Kang ,  Sang-Hyun Lee ,  Eun-Jeong Lee ,  Tae-Wan Kim ,  Sang-Yup Lee

IPC分类号： A01H5/00 ,  C12N9/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C12P7/62

CPC分类号： C12N9/1025 ,  C12N15/8243 ,  C12P7/625

摘要： The present invention relates to polyhydroxyalkanoate synthase (PHA synthase) mutant originated from Pseudomonas sp. 6-19 (KCTC 11027BP) which can prepare lactate polymer and/or copolymer by using lactyl-CoA as a substrate. The present invention relates to a method for preparing lactate polymer and/or copolymer with the synthase mutant. The polyhydroxyalkanoate synthase mutants of the present invention originated from Pseudomonas sp. 6-19 can efficiently prepare lactate polymer and/or copolymer by using as a substrate lactyl-CoA which is difficult to be used as a substrate by conventional polyhydroxyalkanoate synthase.

摘要翻译： 本发明涉及源于假单胞菌属的聚羟基链烷酸酯合成酶（PHA合酶）突变体。 6-19（KCTC 11027BP），其可以通过使用乳酰辅酶A作为底物制备乳酸聚合物和/或共聚物。 本发明涉及一种用合成酶突变体制备乳酸聚合物和/或共聚物的方法。 本发明的聚羟基链烷酸酯合酶突变体起源于假单胞菌属 6-19可以通过使用难以用作常规聚羟基链烷酸酯合成酶的底物的底物乳酰基辅酶A来有效地制备乳酸聚合物和/或共聚物。

公开(公告)号：US20070277268A1

公开(公告)日：2007-11-29

申请号：US11439517

申请日：2006-05-24

申请人： Jun-Hyeong Cho ,  Si-Jae Park ,  Sang-Yup Lee ,  Yu-Kyung Jung

发明人： Jun-Hyeong Cho ,  Si-Jae Park ,  Sang-Yup Lee ,  Yu-Kyung Jung

IPC分类号： A01H5/00 ,  C12P7/62 ,  C12N1/21 ,  C08L77/06

CPC分类号： C12P7/625 ,  C12N9/10 ,  C12N9/1025 ,  C12N9/93 ,  C12N15/70 ,  C12N15/74 ,  C12N15/8242 ,  C12N15/8243 ,  C12N2500/35 ,  Y02P20/52

摘要： The present invention relates to cells or plants that can produce polylactate or its copolymers and to a method for preparing polylactate or its copolymers using the same. More specifically, cells or plants with the ability to produce polylactate or hydroxyalkanoate-lactate copolymers comprise both a gene encoding an enzyme that converts lactate into lactyl-CoA and a gene encoding polyhydroxyalkanoate (PHA) synthase which uses lactyl-CoA as a substrate. Also described is a method for preparing polylactate or hydroxyalkanoate-lactate copolymers which comprises culturing the cells in a medium containing lactate or lactate and various hydroxyalkanoates or culturing the plants. Effective preparation of hydroxyalkanoate-lactate copolymer which comprises various hydroxyalkanoates as well as polylactate, using the cells or the plants, is disclosed.

摘要翻译： 本发明涉及可产生聚乳酸或其共聚物的细胞或植物，以及使用其制备聚乳酸或其共聚物的方法。 更具体地说，具有产生聚乳酸或羟基链烷酸酯 - 乳酸酯共聚物能力的细胞或植物包括编码将乳酸转化为乳酰基-CoA的酶的基因和使用乳酰CoA作为底物的编码聚羟基链烷酸酯（PHA）合酶的基因。 还描述了制备聚乳酸或羟基链烷酸酯 - 乳酸共聚物的方法，其包括在含有乳酸盐或乳酸盐和各种羟基链烷酸酯的培养基中培养细胞或培养植物。 公开了使用细胞或植物有效制备包含各种羟基链烷酸酯以及聚乳酸的羟基链烷酸酯 - 乳酸共聚物。

公开(公告)号：US09410167B2

公开(公告)日：2016-08-09

申请号：US11439517

申请日：2006-05-24

申请人： Jun-Hyeong Cho ,  Si-Jae Park ,  Sang-Yup Lee ,  Yu-Kyung Jung

发明人： Jun-Hyeong Cho ,  Si-Jae Park ,  Sang-Yup Lee ,  Yu-Kyung Jung

IPC分类号： C12N15/70 ,  C12N15/74 ,  C12P7/62 ,  C12N9/10 ,  C12N9/00 ,  C12N15/82

CPC分类号： C12P7/625 ,  C12N9/10 ,  C12N9/1025 ,  C12N9/93 ,  C12N15/70 ,  C12N15/74 ,  C12N15/8242 ,  C12N15/8243 ,  C12N2500/35 ,  Y02P20/52

摘要： The present invention relates to cells or plants that can produce polylactate or its copolymers and to a method for preparing polylactate or its copolymers using the same. More specifically, cells or plants with the ability to produce polylactate or hydroxyalkanoate-lactate copolymers comprise both a gene encoding an enzyme that converts lactate into lactyl-CoA and a gene encoding polyhydroxyalkanoate (PHA) synthase which uses lactyl-CoA as a substrate. Also described is a method for preparing polylactate or hydroxyalkanoate-lactate copolymers which comprises culturing the cells in a medium containing lactate or lactate and various hydroxyalkanoates or culturing the plants. Effective preparation of hydroxyalkanoate-lactate copolymer which comprises various hydroxyalkanoates as well as polylactate, using the cells or the plants, is disclosed.

摘要翻译： 本发明涉及可产生聚乳酸或其共聚物的细胞或植物，以及使用其制备聚乳酸或其共聚物的方法。 更具体地说，具有产生聚乳酸或羟基链烷酸酯 - 乳酸酯共聚物能力的细胞或植物包括编码将乳酸转化为乳酰基-CoA的酶的基因和使用乳酰CoA作为底物的编码聚羟基链烷酸酯（PHA）合酶的基因。 还描述了制备聚乳酸或羟基链烷酸酯 - 乳酸共聚物的方法，其包括在含有乳酸盐或乳酸盐和各种羟基链烷酸酯的培养基中培养细胞或培养植物。 公开了使用细胞或植物有效制备包含各种羟基链烷酸酯以及聚乳酸的羟基链烷酸酯 - 乳酸共聚物。

公开(公告)号：US08420357B2

公开(公告)日：2013-04-16

申请号：US12676832

申请日：2008-08-08

申请人： Si-Jae Park ,  Tae-wan Kim ,  Hye-Ok Kang ,  Taek-Ho Yang ,  Sang-Yup Lee

发明人： Si-Jae Park ,  Tae-wan Kim ,  Hye-Ok Kang ,  Taek-Ho Yang ,  Sang-Yup Lee

IPC分类号： C12P7/62

CPC分类号： C12N9/1025 ,  C12N9/13 ,  C12Y208/03001

摘要： Provided are a microorganism capable of producing a polylactate (PLA) or PLA copolymer from sucrose and a method of producing the PLA or PLA copolymer from sucrose using the same. A gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate are introduced into a microorganism capable of using sucrose as a substrate, and the microorganism is cultured using sucrose as the substrate, thereby allowing efficient production of a PLA or PLA copolymer.

摘要翻译： 提供能够从蔗糖生产聚乳酸（PLA）或PLA共聚物的微生物以及使用其从蔗糖生产PLA或PLA共聚物的方法。 将使用乳酰基辅酶A作为底物的将乳酸酯转化为乳酰基-CoA的酶的基因和聚羟基链烷酸酯（PHA）合成酶的基因导入到能够使用蔗糖作为底物的微生物中，并使用蔗糖作为培养基 从而能够有效地生产PLA或PLA共聚物。