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1.发明授权
公开(公告)号：US5118625A
公开(公告)日：1992-06-02
申请号：US285032
申请日：1988-12-16
申请人： Shuichi Aiba , Tomohisa Nagasaki , Kohji Uchida
发明人： Shuichi Aiba , Tomohisa Nagasaki , Kohji Uchida
CPC分类号： C12N15/815 , C12N15/68 , Y10S435/911
摘要： The plasmid of the present invention contains Leu 2 gene (.beta.-isopropylmaleate dehydrogenase gene) from Saccharomycopsis lipolytica and an autonomously replicating sequence (hereafter referred to as ARS) and can be a shuttle vector between Escherichia coli and Saccharomycopsis lipolytica since it is produced from shuttle vector pVC 1 of Escherichia coli and Saccharomyces cerevisiae. An exogenous useful gene is inserted into the plasmid of the present invention, namely, shuttle vector to transform Escherichia coli, whereby a large quantity of plasmids are obtained. It becomes possible to produce a useful substance in Saccharomycopsis lipolytica as a host using the plasmid.
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公开(公告)号：US06201594B1
公开(公告)日：2001-03-13
申请号：US09148476
申请日：1998-09-08
申请人： Hiroyuki Kohda , Astuhiro Doi , Kohji Uchida
发明人： Hiroyuki Kohda , Astuhiro Doi , Kohji Uchida
IPC分类号： G03B2752
CPC分类号： H04N1/00259 , G03B27/32 , G03C1/49881 , G03C8/4093 , G03D13/002 , H04N1/00249 , H04N1/00275 , H04N1/032
摘要： An image recording apparatus which effects digital exposure while aiming for compactness. An exposure unit digitally exposes a photosensitive material successively while moving above a stage. An application unit moves from the rear side of the exposure unit and applies water successively to the photosensitive material. A superposing unit moves from the rear side of the application unit, and successively superposes an image-receiving material on the photosensitive material. Heat development transfer is carried out, and an image is obtained on the image-receiving material.
摘要翻译： 一种在瞄准紧凑性的同时实现数字曝光的图像记录装置。 曝光单元在舞台上方移动时，依次数字地曝光感光材料。 应用单元从曝光单元的后侧移动并依次向感光材料施加水。 叠加单元从施加单元的后侧移动，并且将感光材料上的图像接收材料相继叠置。 进行热显影转印，并且在图像接收材料上获得图像。
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公开(公告)号：US6115106A
公开(公告)日：2000-09-05
申请号：US15526
申请日：1998-01-29
申请人： Hiroyuki Kohda , Kohji Uchida , Atsuhiro Doi , Yasuhiro Endo
发明人： Hiroyuki Kohda , Kohji Uchida , Atsuhiro Doi , Yasuhiro Endo
CPC分类号： G03B27/32
摘要： An image recording apparatus, in which a necessary place can be suitably exposed by simple operation and the workability of inspection, maintenance and the like improves, is obtained. In the image recording apparatus, an upper cover is rotatably connected to and supported at a base stand by an intermediate hinge. Accordingly, there are a first stage opened/closed state, in which the upper cover opens and closes around a supporting shaft, and a second stage opened/closed state, in which the upper cover integrally opens and closes along with the intermediate hinge around another supporting shaft. In the second stage opened state, the inner portion of the base stand is exposed by a large amount, the distal end portion of a stage is lifted, and further, the holding of a supply magazine and a take-up magazine is released. As a result, the innermost place within the apparatus is exposed and the workability of inspection and maintenance, such as replacement of the supply magazine or the take-up magazine, improves.
摘要翻译： 可以获得能够通过简单的操作适当地曝光必要的地方，检查，维护等的可操作性提高的图像记录装置。 在图像记录装置中，上盖通过中间铰链可转动地连接到支架上并被支撑在基架上。 因此，存在第一阶段打开/关闭状态，其中上盖围绕支撑轴打开和关闭，以及第二阶段打开/关闭状态，其中上盖与中间铰链一起打开和关闭，并且围绕另一状态 支撑轴。 在第二阶段打开状态下，基座的内部被大量暴露，台架的前端部被提起，并且进一步释放供料盒和卷取盒的保持。 结果，设备内的最内部被暴露，并且改进了供应盒或卷取盒的更换的检查和维护的可操作性。
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公开(公告)号：US5827710A
公开(公告)日：1998-10-27
申请号：US869506
申请日：1997-06-05
申请人： Kohji Uchida , Hirokazu Matsukawa , Tuyosi Fujita , Yushi Matuo
发明人： Kohji Uchida , Hirokazu Matsukawa , Tuyosi Fujita , Yushi Matuo
CPC分类号： C12N9/0006 , C12Y101/01027 , C12Y101/01028
摘要： The present invention provides a gene encoding the type B subunit protein of avian lactate dehydrogenase. The gene encoding the type B subunit protein of avian lactate dehydrogenase of the present invention is obtained by plaque hybridization or the like from a cDNA library derived from avian heart muscle and encodes the type B subunit protein of avian lactate dehydrogenase having the sequences shown as SEQ ID NO. 1 or 2.
摘要翻译： 本发明提供编码禽类乳酸脱氢酶的B型亚基蛋白的基因。 编码本发明禽类乳酸脱氢酶B型亚基蛋白的基因是通过来自禽心肌的cDNA文库的斑块杂交等获得的，并编码具有SEQ所示序列的禽类乳酸脱氢酶的B亚基蛋白 证件号码。 1或2。
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5.发明授权Method for producing DNA nested deletions by an in vitro reaction using transposase 失效通过使用转座酶的体外反应产生DNA嵌套缺失的方法公开(公告)号：US06265159B1
公开(公告)日：2001-07-24
申请号：US08998712
申请日：1997-12-29
申请人： Yoshinobu Sugino , Masayuki Morita , Yushi Matuo , Kohji Uchida
发明人： Yoshinobu Sugino , Masayuki Morita , Yushi Matuo , Kohji Uchida
IPC分类号： C12Q168
CPC分类号： C12N15/00
摘要： The present invention provides a method for producing nested deletions in vitro in desired DNA, comprising: 1) preparing a vector containing a DNA fragment in which nested deletions are to be generated and the terminal repeat of a transposon; 2) incubating the vector in vitro with transposase and a DNA replication system; 3) obtaining the vector incorporating the DNA fragment with nested deletions as a reaction product; and optionally, 4) transforming a host cell with the reaction product and growing it; and 5) recovering a vector incorporating said DNA fragment with nested deletions from the grown host cell.
摘要翻译： 本发明提供了一种用于在所需DNA中体外产生嵌套缺失的方法，包括：1）制备含有其中产生嵌套缺失的DNA片段和转座子末端重复的载体; 2）体外培养载体 具有转座酶和DNA复制体系; 3）获得含有嵌合缺失的DNA片段作为反应产物的载体; 和任选地，4）用反应产物转化宿主细胞并使其生长; 和5）从生长的宿主细胞中回收含有嵌套缺失的所述DNA片段的载体。
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公开(公告)号：US5789186A
公开(公告)日：1998-08-04
申请号：US656934
申请日：1996-06-03
申请人： Hitoshi Takeshita , Hiroshi Morita , Akira Fujiwara , Yasutaka Kaneda , Tohru Watanabe , Taizou Hayashi , Kohji Uchida , Yushi Matsuo , Takekazu Horio
发明人： Hitoshi Takeshita , Hiroshi Morita , Akira Fujiwara , Yasutaka Kaneda , Tohru Watanabe , Taizou Hayashi , Kohji Uchida , Yushi Matsuo , Takekazu Horio
CPC分类号： G01N33/6896 , C12Q1/533 , G01N2800/2871
摘要： A sample is treated with a phosphoglyceric acid mutase (PGAM) inhibitor (polythionic acid (salt) such as potassium tetrathionate) to inactivate the M-type isozyme activity and the B-type PGAM is quantified by determining PGAM isozymes by a rate assay. The B-type PGAM is a novel marker for cerebral apoplexy, and the diagnosis of cerebral apoplexy is enabled by assaying it.
摘要翻译： 用磷酸甘油酸变位酶（PGAM）抑制剂（多硫酸（盐）如四硫代硫酸钾）处理样品以失活M型同工酶活性，并通过速率测定法测定PGAM同功酶来定量B型PGAM。 B型PGAM是脑中风的新型标志物，通过测定脑卒中的诊断能力。
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7.发明申请公开(公告)号：US20050120959A1
公开(公告)日：2005-06-09
申请号：US10952829
申请日：2004-09-30
申请人： Yuji Isoda , Kohji Uchida
发明人： Yuji Isoda , Kohji Uchida
CPC分类号： C23C14/505 , C09K11/7733 , C23C14/0694 , C23C14/24 , G21K4/00 , Y02P20/149
摘要： The vacuum deposition device includes a vacuum deposition chamber, a vacuum evacuator for evacuating an inside of the chamber, an evaporating unit for evaporating one or more film forming materials in the chamber, a holding unit provided above the evaporating unit for holding a substrate for the vacuum deposition, and a preventing unit for preventing evaporated particles of the one or more materials from adhering to the substrate when the vacuum deposition is carried out at a pressure of 0.05 to 10 Pa. The pretreatment method for the vacuum deposition prepares the prevention unit in the chamber and melts the one or more materials by heating in the chamber at the pressure of 0.05 to 10 Pa while preventing the evaporated particles from adhering to the substrate by using the preventing unit.
摘要翻译： 真空沉积装置包括真空沉积室，用于抽空室内的真空抽气机，用于蒸发腔室中的一种或多种成膜材料的蒸发单元，设置在蒸发单元上方的保持单元的保持单元，用于保持用于 真空沉积以及当在0.05〜10Pa的压力下进行真空蒸镀时，能够防止一种或多种材料的蒸发的粒子附着在基板上的防止单元，进行真空蒸镀的预处理方法， 并且通过在0.05至10Pa的压力下在室中加热来熔化一种或多种材料，同时通过使用防止单元防止蒸发的颗粒粘附到基板。
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公开(公告)号：US6163370A
公开(公告)日：2000-12-19
申请号：US149016
申请日：1998-09-08
申请人： Hiroyuki Kohda , Astuhiro Doi , Kohji Uchida
发明人： Hiroyuki Kohda , Astuhiro Doi , Kohji Uchida
CPC分类号： H04N1/00259 , G03B27/32 , G03C1/49881 , G03C8/4093 , G03D13/002 , H04N1/00249 , H04N1/00275 , H04N1/032
摘要： An image recording apparatus which effects digital exposure while aiming for compactness. An exposure unit digitally exposes a photosensitive material successively while moving above a stage. An application unit moves from the rear side of the exposure unit and applies water successively to the photosensitive material. A superposing unit moves from the rear side of the application unit, and successively superposes an image-receiving material on the photosensitive material. Heat development transfer is carried out, and an image is obtained on the image-receiving material.
摘要翻译： 一种在瞄准紧凑性的同时实现数字曝光的图像记录装置。 曝光单元在舞台上方移动时，依次数字地曝光感光材料。 应用单元从曝光单元的后侧移动并依次向感光材料施加水。 叠加单元从施加单元的后侧移动，并且将感光材料上的图像接收材料相继叠置。 进行热显影转印，并且在图像接收材料上获得图像。
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公开(公告)号：US6145830A
公开(公告)日：2000-11-14
申请号：US007764
申请日：1998-01-15
申请人： Kohji Uchida , Hiroyuki Kohda , Yasuhiro Endo , Atsuhiro Doi
发明人： Kohji Uchida , Hiroyuki Kohda , Yasuhiro Endo , Atsuhiro Doi
CPC分类号： B65H3/0833 , B65H2301/42346 , B65H2403/512 , B65H2405/1117 , B65H2405/13
摘要： A sheet material supplying apparatus having a low-cost sucker adsorbing unit of a simple structure is provided for the carrying of sheet materials that are used under a high-humidity environment or that include humidity, to ensure a proper separation of each sheet material. Sheet materials accommodated in a tray are being sandwiched between a push-up panel and an engagement claw. At the time of taking out the sheet materials from the tray, the push-up panel is pressed down by a cam to cancel the state of sandwiching between the engagement claw and the push-up panel. Accordingly, only the top layer sheet material is taken out from the tray, with the rest of the lower layer sheet materials being dropped by their self-weight. Further, the sheet materials are carried by the sucker adsorbing unit. When a sucker adsorbing a sheet material by a negative pressure has moved to a carrying roller, a sucker supporting section is elastically deformed by a sucker pressing unit, so that the negative pressure within the sucker can be cancelled. Accordingly, a complex control system is not required for cancelling the negative pressure.
摘要翻译： 提供一种具有简单结构的低成本抽吸吸附单元的片材供应装置，用于承载在高湿度环境下使用或包括湿度的片材，以确保每个片材的适当分离。 容纳在托盘中的片材夹在上推面板和接合爪之间。 从托盘取出片状材料时，上推面板被凸轮按压，以抵消接合爪和上推面板之间夹持的状态。 因此，只有顶层片材从托盘中取出，其余的下层片材由于其自重而下降。 此外，片材由吸盘吸附单元承载。 当通过负压吸附片材的吸盘已经移动到搬运辊时，吸盘支撑部通过吸盘单元弹性变形，从而可以消除吸盘内的负压。 因此，不需要复杂的控制系统来消除负压。
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公开(公告)号：US6057141A
公开(公告)日：2000-05-02
申请号：US128967
申请日：1998-08-04
申请人： Kohji Uchida , Hirokazu Matsukawa , Tuyosi Fujita , Yushi Matuo
发明人： Kohji Uchida , Hirokazu Matsukawa , Tuyosi Fujita , Yushi Matuo
CPC分类号： C12N9/0006 , C12Y101/01027 , C12Y101/01028
摘要： The present invention provides a gene encoding the type B subunit protein of avian lactate dehydrogenase. The gene encoding the type B subunit protein of avian lactate dehydrogenase of the present invention is obtained by plaque hybridization or the like from a cDNA library derived from avian heart muscle and encodes the type B subunit protein of avian lactate dehydrogenase having an amino acid sequence shown as SEQ ID NO. 2.
摘要翻译： 本发明提供编码禽类乳酸脱氢酶的B型亚基蛋白的基因。 本发明的编码禽类乳酸脱氢酶B型亚单位蛋白的基因是通过来自禽心肌的cDNA文库的斑块杂交等获得的，并编码具有氨基酸序列的禽类乳酸脱氢酶的B亚基蛋白 如SEQ ID NO。 2。
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