公开(公告)号：US5377676A

公开(公告)日：1995-01-03

申请号：US39991

申请日：1993-03-30

Applicant: Sandor G. Vari ,  Warren S. Grundfest

Inventor： Sandor G. Vari ,  Warren S. Grundfest

IPC: A61B5/00 ,  A61B10/00 ,  A61B10/02 ,  A61B17/00 ,  A61B17/22 ,  A61B18/22 ,  A61B19/00 ,  G01N21/64

CPC classification number: A61B10/0233 ,  A61B18/22 ,  A61B5/0059 ,  A61B5/415 ,  A61B5/416 ,  A61B5/418 ,  G01N21/6428 ,  A61B2017/00061 ,  A61B2017/22087 ,  A61B2090/062 ,  A61B2090/306 ,  A61B2090/3941

Abstract: A method is disclosed for determining the biodistribution of substances using fluorescence spectroscopy. A photosensitizing agent or other intrinsically fluorescent agent, or an agent labeled with an extrinsic fluorophor is administered to a subject. A fiberoptic probe integrated with an excitation light source illuminates the examined tissue and causes fluorescence. The fluorescence is recorded by a spectrograph and plotted as a spectral curve. The intensity ratio (S1/S2) for the fluorescence from the photosensitizing agent (S1) and autofluorescence (S2) for the examined tissue is used as an index for drug presence and compared with the intensity ratio at the same wavelengths for various tissues.

Abstract translation: 公开了使用荧光光谱测定物质生物分布的方法。 向受试者施用光敏剂或其它固有荧光剂或者用外在荧光素标记的药剂。 与激发光源集成的光纤探头照亮被检查的组织并引起荧光。 通过光谱仪记录荧光，作为光谱曲线绘制。 使用来自光敏剂（S1）的荧光和检测组织的自发荧光（S2）的强度比（S1 / S2）作为药物存在的指标，并与各种组织的相同波长的强度比进行比较。

公开(公告)号：US5503559A

公开(公告)日：1996-04-02

申请号：US129408

申请日：1993-09-30

Applicant: Sandor G. Vari

Inventor： Sandor G. Vari

IPC: A61B5/00 ,  A61C5/02 ,  A61C13/15 ,  A61C19/04 ,  A61L2/10 ,  A61C5/10 ,  A61C1/00

CPC classification number: A61B5/0088 ,  A61C19/004 ,  A61C5/02 ,  A61L2/10

Abstract: An endodontic apparatus and related method for the instant detection of the anatomical structure of a tooth and for the restoration of a dead or severely decayed tooth using induced fluorescence spectroscopy. A root canal probe having an optical fiber through its center transmits excitation light into the tooth's root canal. The excitation light induces the tissue within the root canal to fluoresce. The fluorescent light is collected by the optical fiber and transmitted back to a sensor that generates electrical signals indicative of the intensity of light within predetermined wavelength bands. The electrical signals are processed to identify the tissues within the root canal. The probe may include a slightly conical metal surface having flutes for shaving and removing dentin from within the root canal. Using the fluorescent emission properties of the tissues of components of a tooth, the entrance of the root canal is located. The root canal is cleaned and shaped and the apex of the root canal located using the difference between the fluorescence spectrum of the apex and the root canal. The root canal is sealed and filled by a light cure restorative delivered into the root canal through a tube. The light cure restorative is activated by light transmitted into the root canal by an optical fiber. The light activation and polymerization of the light cure restorative can be controlled by monitoring the intensity of the restorative's fluorescence spectrum.

Abstract translation: 用于立即检测牙齿的解剖结构并使用诱导荧光光谱法恢复死亡或严重衰退的牙齿的根管装置和相关方法。 通过其中心具有光纤的根管探针将激发光透射到牙齿的根管中。 激发光诱导根管内的组织发荧光。 荧光灯由光纤收集并传输回传感器，该传感器产生指示预定波长带内的光强度的电信号。 处理电信号以识别根管内的组织。 探针可以包括略微锥形的金属表面，其具有用于剃刮的槽纹和从根管内去除牙本质。 使用牙齿组分的组织的荧光发射特性，定位根管的入口。 根管被清洁和成形，根管的顶点使用顶点的荧光光谱与根管之间的差异来定位。 根管被密封并由通过管输送到根管中的光固化修复剂填充。 光固化修复剂由通过光纤传播到根管中的光激活。 光固化修复剂的光激活和聚合可以通过监测恢复性荧光光谱的强度来控制。

公开(公告)号：US5701902A

公开(公告)日：1997-12-30

申请号：US306118

申请日：1994-09-14

Applicant: Sandor G. Vari ,  Jean-Michel I. Maarek

Inventor： Sandor G. Vari ,  Jean-Michel I. Maarek

IPC: G01N21/64 ,  A61B5/00 ,  A61B5/103 ,  G01N21/35

CPC classification number: A61B5/445 ,  A61B5/0059 ,  A61B5/0071 ,  A61B5/0075

Abstract: A burn evaluation apparatus and related method, that allows a surgeon to make a quick evaluation of the extent and depth of a skin burn injury by employing induced ultraviolet or blue light fluorescence spectroscopy and visible and infrared reflectance spectroscopy. The apparatus monitors the condition of the structural and metabolic constituents in the injured skin. The apparatus includes a plurality of light sources, a sensor, a microprocessor, and several optical fiber bundles. The light sources emit excitation light at predetermined wavelengths, and when each is activated, the sensor measures the amount return light received within several wavelength bands of interest. A side fiber bundle spaced about a centimeter from a main fiber bundle assists in detecting tissue water below the burn area. By optically evaluating the skin at the burn site, the apparatus prevents the unnecessary removal of viable skin that will heal spontaneously within a few weeks, thereby reducing the amount of skin that must be surgically grafted.

Abstract translation: 一种烧伤评估装置及相关方法，其允许外科医生通过使用诱导紫外或蓝光荧光光谱和可见光和红外反射光谱法快速评估皮肤烧伤的程度和深度。 该装置监测受伤皮肤中结构和代谢成分的状况。 该装置包括多个光源，传感器，微处理器和多个光纤束。 光源发射预定波长的激发光，并且当激活光被激活时，传感器测量在感兴趣的几个波长带内接收的返回光量。 与主纤维束间隔约一厘米的侧纤维束有助于检测烧伤区域以下的组织水。 通过光刻评估烧伤部位的皮肤，该装置可防止不必要的去除在几周内自发愈合的活皮肤，从而减少必须手术移植的皮肤的量。

公开(公告)号：US5318023A

公开(公告)日：1994-06-07

申请号：US679766

申请日：1991-04-03

Applicant: Sandor G. Vari ,  Theodore Papazoglou ,  Warren S. Grundfest

Inventor： Sandor G. Vari ,  Theodore Papazoglou ,  Warren S. Grundfest

IPC: A61B5/00 ,  A61B10/00 ,  A61B10/02 ,  A61B17/22 ,  A61B18/20 ,  A61B18/22 ,  A61B19/00

CPC classification number: A61B10/0233 ,  A61B18/22 ,  A61B5/0059 ,  A61B5/415 ,  A61B5/418 ,  A61B2017/22087 ,  A61B2090/062

Abstract: A method and apparatus is disclosed for the instant intraoperative detection and biopsy of metastatic cancer using fluorescence spectroscopy. A photosensitizing agent selectively retained by cancerous tissue is administered prior to surgery. A fiberoptic probe integrated with a biopsy device illuminates the examined tissue and causes fluorescence which is recorded by a spectrograph and plotted as a spectral curve. The intensity ratio (S1/S2) for the fluorescence from the photosensitizing agent (S1) and autofluorescence (S2) for the examined tissue is compared with the intensity ratio at the same wavelengths for primary tumor and normal tissue. Tissue that displays an intensity ratio different from that of normal tissue can immediately be analyzed for the depth of tumor involvement and then excised for histological examination using the biopsy device.

Abstract translation: 公开了使用荧光光谱法进行的转移性癌症的即时术中检测和活检的方法和装置。 在手术前给予由癌组织选择性保留的光敏剂。 与活检装置集成的光纤探针照亮被检查的组织并引起荧光，其由光谱仪记录并绘制为光谱曲线。 将来自光敏剂（S1）的荧光和检测组织的自发荧光（S2）的强度比（S1 / S2）与原发肿瘤和正常组织的相同波长的强度比进行比较。 显示与正常组织不同的强度比的组织可以立即分析肿瘤深度，然后用活检装置进行组织学检查。

公开(公告)号：US5456252A

公开(公告)日：1995-10-10

申请号：US129406

申请日：1993-09-30

Applicant: Sandor G. Vari ,  Theodore Papazoglou ,  Warren S. Grundfest

Inventor： Sandor G. Vari ,  Theodore Papazoglou ,  Warren S. Grundfest

IPC: A61B5/00 ,  G01N21/64 ,  A61B6/00

CPC classification number: G01N21/6486 ,  G01N2021/6417 ,  G01N2021/6484 ,  Y10S128/925

Abstract: A medical monitor, and related method, determines a pre-existing physiological property of an organ of a patient by monitoring fluorescent light produced by constituents associated with the metabolic and structural condition of the organ. The monitor illuminates the organ with ultraviolet excitation light that induces some constituents of the organ to fluoresce, with the fluorescent light being monitored and processed to determine pre-existing physiological properties of the organ. A sensor monitors the return light, which includes fluorescent light produced by the fluorescent constituents of the organ, and generates first and second electrical signals indicative of the intensity of light at two wavelength. One wavelength is associated with the fluorescence of collagen, a constituent associated with organ's structural properties, and the other wavelength is a associated with the fluorescence of NADH, a constituent associated with the organ's metabolism. A processor then processes the first and second electrical signals to determine the localized pH of the organ. A fiber-optic waveguide is used to guide the excitation light from the laser light source to the organ and the return light from the organ to the sensor. In another aspect of the invention, the sensor generates a third electrical signal associated with the fluorescence of elastin, a constituent associated with the organ's structural properties. The processor processes the first, second and third electrical signals to determine the perfusion or oxygenation of the organ.

Abstract translation: 医疗监视器和相关方法通过监测与器官的代谢和结构状况相关的成分产生的荧光，来确定患者器官的预先存在的生理特性。 监视器用紫外线激发光照亮器官，诱导器官的一些成分发荧光，荧光被监测和处理以确定器官的先前存在的生理特性。 传感器监测返回光，其包括由器官的荧光成分产生的荧光，并产生指示两个波长的光强度的第一和第二电信号。 一个波长与胶原蛋白的荧光相关联，胶原蛋白是与器官结构特性相关的成分，另一个波长与NADH（与器官代谢相关的成分）的荧光相关。 然后处理器处理第一和第二电信号以确定器官的局部pH。 使用光纤波导将来自激光光源的激发光引导至器官，并将来自器官的返回光引导至传感器。 在本发明的另一方面，传感器产生与弹性蛋白的荧光相关联的第三电信号，弹性蛋白是与器官结构特性相关的成分。 处理器处理第一，第二和第三电信号以确定器官的灌注或氧合。