Abstract:
A method of analyzing nucleic acid by compensating for crosstalk in polymerase chain reaction (PCR) data and other data, wherein crosstalk signals associated with multiple fluorescent dyes are corrected by using fluorescent intensity variations detected from a concentration difference of the fluorescent dyes, and apparatus for performing the method.
Abstract:
A method and apparatus for performing quantitative analysis of a nucleic acid by determining a curve-fitting area based on fluorescence intensity data obtained by performing PCR on a target nucleic acid; analyzing parameters for amplification efficiency and nucleic acid concentration by curve-fitting a result of performing PCR on a reference nucleic acid with a known initial nucleic acid concentration; and estimating the initial nucleic acid concentration of the target nucleic acid by performing curve-fitting on the determined curve-fitting area using the analyzed parameters.
Abstract:
A method for amplifying a nucleic acid amplification in the presence of an ionic liquid that suppresses an inhibitor of nucleic acid amplification, particularly when in a biological material, and a composition useful for performing the method.
Abstract:
A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber
Abstract:
A composition and kit for detecting Clostridium difficile including a primer set for detecting a strain of Clostridium difficile, and a method of detecting Clostridium difficile by using the same.