METHOD FOR REVERSIBLE FIXATION OR SELECTIVE LYSIS OF CELL USING PHOTOCLEAVABLE POLYMER
    3.
    发明申请
    METHOD FOR REVERSIBLE FIXATION OR SELECTIVE LYSIS OF CELL USING PHOTOCLEAVABLE POLYMER 有权
    使用光可溶性聚合物进行反向固定或选择性细胞分析的方法

    公开(公告)号:US20150148243A1

    公开(公告)日:2015-05-28

    申请号:US14323812

    申请日:2014-07-03

    CPC classification number: G01N1/30 G01N2001/305

    Abstract: A photocleavable polymer comprising a photocleavable linker and a polymer having a functional group that binds to a protein, lipid, sugar of a cell, or any combination thereof, as well as a method of selectively lysing cells by incubating a sample comprising two or more cells with the photocleavable polymer to reversibly fix the cells; selectively irradiating the fixed cells or a target or non-target cell among the fixed cells to cleave the photocleavable polymer; and adding a cell lysis solution to selectively lyse the irradiated cells.

    Abstract translation: 包含可光切割接头和具有结合蛋白质,脂质,细胞糖或其任何组合的官能团的聚合物的可光致透照聚合物,以及通过将包含两个或多个细胞的样品 用可透光聚合物可逆地固定细胞; 选择性地照射固定细胞中的固定细胞或靶或非靶细胞以切割可光裂解的聚合物; 并加入细胞裂解溶液以选择性地裂解照射的细胞。

    PARTICLE COMPLEX AND METHOD OF ISOLATING TARGET CELL
    6.
    发明申请
    PARTICLE COMPLEX AND METHOD OF ISOLATING TARGET CELL 审中-公开
    颗粒复合物和分离目标细胞的方法

    公开(公告)号:US20140011192A1

    公开(公告)日:2014-01-09

    申请号:US13774887

    申请日:2013-02-22

    CPC classification number: G01N21/6486 G01N33/54313

    Abstract: A particle complex comprising a particle; a cleavable linker bound to a surface of the particle; and a macromolecule bound to the cleavable linker, wherein the macromolecule specifically binds to a surface marker of a target cell, and a method of isolating a target cell comprising same.

    Abstract translation: 包含颗粒的颗粒复合物; 与所述颗粒的表面结合的可切割连接体; 以及与可切割接头结合的大分子,其中所述大分子特异性结合靶细胞的表面标志物,以及分离包含其的靶细胞的方法。

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