公开(公告)号：US20150037801A1

公开(公告)日：2015-02-05

申请号：US14180931

申请日：2014-02-14

Applicant: Samsung Electronics Co., Ltd.

Inventor： Dong-hyun PARK ,  Yeon-jeong Kim ,  Kyung-yeon Han

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q2521/537 ,  C12Q2523/301

Abstract: Provided is a method of amplifying a nucleic acid of a cell involving applying ultrasonic waves to lyse a cell bound to a solid support to provide a cell lysate comprising a nucleic acid; separating the cell lysate from the solid support; adding a protease to the cell lysate; and amplifying the nucleic acid.

Abstract translation: 提供一种扩增细胞核酸的方法，所述方法包括施加超声波以裂解与固体支持物结合的细胞，以提供包含核酸的细胞裂解物; 将细胞裂解物与固体支持物分离; 向细胞裂解物中加入蛋白酶; 并扩增核酸。

公开(公告)号：US20150031023A1

公开(公告)日：2015-01-29

申请号：US14189762

申请日：2014-02-25

Applicant: Samsung Electronics Co., Ltd.

Inventor： Dong-hyun PARK ,  Yeon-jeong Kim ,  Kyung-yeon Han

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q2537/163

Abstract: Provided are a composition or a kit for detecting a nucleic acid with genetic variation including a first amplification blocking nucleic acid and a second amplification blocking nucleic acid, and a method of detecting a nucleic acid with genetic variation by using the same. Based on the above, a nucleic acid with genetic variation can be detected with high sensitivity and accuracy.

Abstract translation: 提供了用于检测具有遗传变异的核酸的组合物或试剂盒，包括第一扩增阻断核酸和第二扩增阻断核酸，以及通过使用该核酸检测具有遗传变异的核酸的方法。 基于上述，可以以高灵敏度和准确度检测出具有遗传变异的核酸。

公开(公告)号：US20140141418A1

公开(公告)日：2014-05-22

申请号：US13899223

申请日：2013-05-21

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Dong-hyun PARK ,  Sung-woo HONG ,  Kyung-hee PARK ,  Myo-yong LEE

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6844 ,  C12Q2525/207 ,  C12Q2525/307 ,  C12Q2531/119

Abstract: Provided is a dual-hybridization polynucleotide including a first complementary region that is complementary to the 3′-terminus of a target nucleic and a second complementary region that is complementary to the 5′-terminus of the target nucleic acid, a composition and kit including the polynucleotide, and a method of producing a nucleotide sequence complementary to the target nucleic acid. The first complementary region to be bound at the 3′-terminus of the target nucleic acid can be shortened and the target nucleic acid may be amplified with excellent specificity and/or sensitivity.

Abstract translation: 提供了双杂交多核苷酸，其包含与靶核酸的3'末端互补的第一互补区和与靶核酸的5'末端互补的第2互补区，包含 多核苷酸，以及产生与靶核酸互补的核苷酸序列的方法。 可以缩短在靶核酸的3'-末端结合的第一个互补区，并且以优异的特异性和/或灵敏度扩增靶核酸。

公开(公告)号：US20130302822A1

公开(公告)日：2013-11-14

申请号：US13768680

申请日：2013-02-15

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Myoung-soon KIM ,  Dong-hyun PARK ,  Chang-eun YOO ,  Ga-hee KIM

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N33/56966 ,  G01N2333/70596

Abstract: A method of analyzing exosomes, the method comprising incubating a sample comprising fluorescence-labeled exosomes with a solid support so that the fluorescence-labeled exosomes bind to the solid support; measuring a fluorescence signal from the bound fluorescence-labeled exosomes; and analyzing the exosomes based on the measured fluorescence signal.

Abstract translation: 一种分析外来体的方法，所述方法包括将包含荧光标记的外来体的样品与固体支持物孵育，使得荧光标记的外来体与固体支持物结合; 从结合的荧光标记的外来体测量荧光信号; 并根据测量的荧光信号分析外来体。

公开(公告)号：US20130157300A1

公开(公告)日：2013-06-20

申请号：US13678357

申请日：2012-11-15

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Dong-hyun PARK ,  Hyun-ju KANG ,  Myo-yong LEE

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  C12Q1/34 ,  C12Q1/37 ,  G01N21/6428 ,  G01N2500/10

Abstract: A method of detecting and recovering of exosomes in a sample, as well as a method of determining the recovery rate of exosomal recovery, and a method of screening for a material that induces secretion of the exosomes in a sample, which methods employ the use of a protease.

Abstract translation: 一种检测和回收样品中外来体的方法，以及确定外来物质回收率回收率的方法，以及筛选诱导样品中外来体分泌物质的方法，该方法采用 一种蛋白酶。