公开(公告)号：US11491489B2

公开(公告)日：2022-11-08

申请号：US16231272

申请日：2018-12-21

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Marco Angelo Bianchessi ,  Lillo Raia

IPC: B01L3/00 ,  G01N35/10 ,  G01N35/00 ,  F16L21/03

Abstract: A microfluidic group includes a female connector and a male needle connector. The female connector has a connector chamber in a containment body; a duct extending in the containment body to a duct opening on a first face of the connector chamber; a needle entry hole extending from a lateral face of the containment body to a second face, not facing the first face of the connector chamber; and a gasket arranged in the connector chamber. The gasket has a side wall internally delimiting a cavity and extending in part adjacent to the second face of the connector chamber. The cavity of the gasket faces the first face of the connector chamber.

公开(公告)号：US10190161B2

公开(公告)日：2019-01-29

申请号：US14676618

申请日：2015-04-01

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Marco Angelo Bianchessi ,  Francesco Ferrara

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  B82Y30/00

Abstract: An apparatus for nucleic acid sequencing includes: a base-detection device in a detection site, the base-detection device being configured to detect bases of a portion of a nucleic acid strand at the detection site; and a conveying device, configured to extend the nucleic acid strand and to cause the extended nucleic acid strand to slide through the detection site along a path. The base-detection device includes a plurality of field-effect nanowire detectors, arranged along the path and each including a respective nanowire and nucleic acid probes, which are defined by respective base sequences and are fixed to the respective nanowire.

公开(公告)号：US20170369943A1

公开(公告)日：2017-12-28

申请号：US15394371

申请日：2016-12-29

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Alessandro Paolo Bramanti ,  Marco Angelo Bianchessi

IPC: C12Q1/68

Abstract: A method for sequencing a nucleic acid strand, comprising the steps of: providing a solution containing truncated strands having lengths different from one another terminating with a respective dideoxynucleotide from among ddATP, ddTTP, ddGTP, and ddCTP; functionalizing first masses by a donor molecule and second masses by an acceptor molecule such as to generate a light emission when they come into mutual contact; coupling a first mass to a first end of each truncated strand; coupling the second masses to a respective terminal dideoxynucleotide of each strand; applying an AC electrical field having variable frequencies that are such as to generate, on each second mass, a net movement directed towards the first mass; acquiring a plurality of light radiations for each frequency value; and associating each light radiation acquired to a respective dideoxynucleotide and, thus, to a respective nucleotide base.

公开(公告)号：US09765326B2

公开(公告)日：2017-09-19

申请号：US14676626

申请日：2015-04-01

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Francesco Ferrara ,  Marco Angelo Bianchessi

IPC: G01N27/447 ,  C12N15/10 ,  B01L3/00 ,  G01N33/487 ,  C12Q1/68

CPC classification number: C12N15/101 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0627 ,  C12Q1/6869 ,  C12Q2523/307 ,  C12Q2563/157 ,  C12Q2565/607 ,  G01N27/44791 ,  G01N33/48721

Abstract: An apparatus for nucleic acid sequencing includes a nanochannel and a conveying device, configured to move a nucleic acid strand through the nanochannel. The conveying device includes: a first electrode, a second electrode, and a third electrode, which are arranged along the nanochannel so as to be in contact with a fluid occupying the nanochannel, the second electrode being arranged between the first electrode and the third electrode; and a control unit configured to apply a first voltage, a second voltage, and a third voltage, respectively, to the first electrode, the second electrode and the third electrode, for controlling movement of the nucleic acid strand through the nanochannel.

公开(公告)号：US10597716B2

公开(公告)日：2020-03-24

申请号：US15394371

申请日：2016-12-29

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Alessandro Paolo Bramanti ,  Marco Angelo Bianchessi

IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: A method for sequencing a nucleic acid strand, comprising the steps of: providing a solution containing truncated strands having lengths different from one another terminating with a respective dideoxynucleotide from among ddATP, ddTTP, ddGTP, and ddCTP; functionalizing first masses by a donor molecule and second masses by an acceptor molecule such as to generate a light emission when they come into mutual contact; coupling a first mass to a first end of each truncated strand; coupling the second masses to a respective terminal dideoxynucleotide of each strand; applying an AC electrical field having variable frequencies that are such as to generate, on each second mass, a net movement directed towards the first mass; acquiring a plurality of light radiations for each frequency value; and associating each light radiation acquired to a respective dideoxynucleotide and, thus, to a respective nucleotide base.

公开(公告)号：US11278897B2

公开(公告)日：2022-03-22

申请号：US16224626

申请日：2018-12-18

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Davide Cucchi ,  Marco Angelo Bianchessi ,  Alessandro Cocci ,  Lillo Raia ,  Lorenzo Bruno ,  Nadia Serina ,  Marco Cereda ,  Danilo Pirola ,  Pietro Ferrari ,  Francesco Ferrara ,  Alessandro Paolo Bramanti

IPC: B01L3/00 ,  C12Q1/6806 ,  G01N1/34

Abstract: A sample treatment and molecule analysis cartridge is configured to be mounted in a treatment machine vertically. The cartridge has a sample inlet opening, a fluidic inlet, and a fluidic outlet. The cartridge houses an extraction chamber extending vertically from the sample inlet opening and connected to the fluidic inlet; a waste chamber extending vertically, alongside the extraction chamber; and a collector extending along the extraction chamber and the waste chamber and having a smaller height than the extraction chamber and the waste chamber. A fluidic circuit connects together the extraction chamber, the waste chamber, the collector, the fluidic inlet, and the fluidic outlet, and is configured to connect the fluidic outlet to vent openings of the extraction chamber, the waste chamber, and the collector, and to connect the bottom end of the extraction chamber to the fluidic inlet, the waste chamber, and the collector.

公开(公告)号：US20150284792A1

公开(公告)日：2015-10-08

申请号：US14676618

申请日：2015-04-01

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Marco Angelo Bianchessi ,  Francesco Ferrara

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B82Y30/00 ,  G01N27/4146

Abstract: An apparatus for nucleic acid sequencing includes: a base-detection device in a detection site, the base-detection device being configured to detect bases of a portion of a nucleic acid strand at the detection site; and a conveying device, configured to extend the nucleic acid strand and to cause the extended nucleic acid strand to slide through the detection site along a path. The base-detection device includes a plurality of field-effect nanowire detectors, arranged along the path and each including a respective nanowire and nucleic acid probes, which are defined by respective base sequences and are fixed to the respective nanowire.

Abstract translation: 用于核酸测序的装置包括：检测位点中的碱基检测装置，所述碱基检测装置被配置为检测所述检测部位的核酸链的一部分的碱基; 以及传送装置，其被配置为延伸所述核酸链并且使所述延伸的核酸链沿着路径滑动通过所述检测部位。 基底检测装置包括多个场效应纳米线检测器，沿着路径布置，每个包括相应的纳米线和核酸探针，它们由相应的碱基序列限定并固定在相应的纳米线上。

公开(公告)号：US20150284707A1

公开(公告)日：2015-10-08

申请号：US14676626

申请日：2015-04-01

Applicant: STMICROELECTRONICS S.R.L.

Inventor： Francesco Ferrara ,  Marco Angelo Bianchessi

IPC: C12N15/10 ,  B01L3/00 ,  G01N27/447

CPC classification number: C12N15/101 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0627 ,  C12Q1/6869 ,  C12Q2523/307 ,  C12Q2563/157 ,  C12Q2565/607 ,  G01N27/44791 ,  G01N33/48721

Abstract: An apparatus for nucleic acid sequencing includes a nanochannel and a conveying device, configured to move a nucleic acid strand through the nanochannel. The conveying device includes: a first electrode, a second electrode, and a third electrode, which are arranged along the nanochannel so as to be in contact with a fluid occupying the nanochannel, the second electrode being arranged between the first electrode and the third electrode; and a control unit configured to apply a first voltage, a second voltage, and a third voltage, respectively, to the first electrode, the second electrode and the third electrode, for controlling movement of the nucleic acid strand through the nanochannel.

Abstract translation: 用于核酸测序的装置包括纳米通道和输送装置，其被配置成使核酸链通过纳米通道移动。 输送装置包括：第一电极，第二电极和第三电极，其沿着纳米通道布置成与占据纳米通道的流体接触，第二电极布置在第一电极和第三电极之间 ; 以及控制单元，被配置为分别向第一电极，第二电极和第三电极施加第一电压，第二电压和第三电压，以控制核酸链通过纳米通道的运动。