公开(公告)号：US09476090B2

公开(公告)日：2016-10-25

申请号：US14283993

申请日：2014-05-21

Applicant: STC.UNM

Inventor： Carl Brown, III ,  Steven Wayde Graves ,  Darko Stefanovic ,  Matthew Richard Lakin

IPC: C07H21/04 ,  C12Q1/68 ,  C07H21/02

CPC classification number: C12Q1/6816 ,  C12Q2521/345 ,  C12Q2525/301 ,  C12Q2565/1015

Abstract: This disclosure describes a structured polynucleotide, devices that include the structured polynucleotide, and methods involving the structured polynucleotide and/or devices. Generally, the structured polynucleotide includes five domains. A first domain acts as a toehold for an input DNA logic gate to initiate binding to an SCS biomolecule. A second domain acts as a substrate recognition sequence for an upstream DNA logic gate. A third domain acts as a toehold for a output DNA logic gate to initiate binding of the SCS biomolecule to the gate. A fourth domain acts as an effector sequence to alter the state of the output logic gate. A fifth domain acts as a cage sequence to lock the effector sequence in an inactive state until an input gate binds to the structured polynucleotide.

Abstract translation: 本公开描述了结构化多核苷酸，包括结构化多核苷酸的装置和涉及结构化多核苷酸和/或装置的方法。 通常，结构化多核苷酸包括五个结构域。 第一个域用作输入DNA逻辑门的始发点，以启动与SCS生物分子的结合。 第二个域用作上游DNA逻辑门的底物识别序列。 第三个域用作输出DNA逻辑门的脚注，以启动SCS生物分子与门的结合。 第四个域用作效应序列来改变输出逻辑门的状态。 第五结构域充当笼状序列，以将效应序列锁定在无活性状态，直到输入门结合结构化多核苷酸。