公开(公告)号：US20160024147A1

公开(公告)日：2016-01-28

申请号：US14808171

申请日：2015-07-24

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： Andrew TUSTIAN ,  Christine ENDICOTT ,  Benjamin ADAMS ,  John MATTILA ,  Hanne BAK

IPC: C07K1/22 ,  B01D15/38 ,  B01J39/08 ,  B01J39/26 ,  C07K16/12 ,  C07K1/16

CPC classification number: C07K1/22 ,  B01D15/3819 ,  B01D15/3828 ,  B01J39/08 ,  B01J39/26 ,  C07K16/065 ,  C07K2317/14 ,  C07K2317/31 ,  C07K2317/526

Abstract: High resolution protein A chromatography employing a chaotropic agent and pH gradient or pH step elution buffer results in improved peak resolution between closely related molecular species. Bispecific antibodies containing a protein A-binding-ablating substitution CH3 domain paired with a protein A-binding CH3 domain are separated with high peak resolution from monospecific antibodies containing a protein A-binding-ablating substituted CH3 domain paired with the protein A-binding-ablating substituted CH3 domain and monospecific antibodies containing a protein A-binding CH3 domain paired with the protein A-binding CH3 domain. Useful chaotropic agents include magnesium chloride and calcium chloride.

Abstract translation: 使用离液剂和pH梯度或pH步骤洗脱缓冲液的高分辨率蛋白质A层析结果提高了密切相关的分子物质之间的峰分辨率。 将含有与蛋白质A结合的CH3结构域配对的蛋白质A结合消融的取代的CH3结构域的双特异性抗体与来自含有蛋白质A结合消融的取代的CH3结构域的单特异性抗体以高蛋白A-结合 - 消融取代的CH3结构域和含有与蛋白A结合CH3结构域配对的蛋白A结合CH3结构域的单特异性抗体。 有用的离液剂包括氯化镁和氯化钙。

公开(公告)号：US20170191101A1

公开(公告)日：2017-07-06

申请号：US15126790

申请日：2015-03-17

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Benjamin ADAMS ,  Andrew TUSTIAN ,  Hanne BAK

IPC: C12P21/00 ,  C12N5/00

CPC classification number: C12P21/00 ,  C12N5/0081

Abstract: Disclosed is an improved method for clarifying a cell culture during the manufacture of a protein. The method includes the step of transiently reducing the pH of a cell culture, followed by a holding step for a period of time, followed by the neutralization of the cell culture prior to clarification by centrifugation.