Invention Application
- Patent Title: CLARIFICATION OF MAMMALIAN CELL CULTURE
-
Application No.: US15126790Application Date: 2015-03-17
-
Publication No.: US20170191101A1Publication Date: 2017-07-06
- Inventor: Benjamin ADAMS , Andrew TUSTIAN , Hanne BAK
- Applicant: Regeneron Pharmaceuticals, Inc.
- International Application: PCT/US15/20862 WO 20150317
- Main IPC: C12P21/00
- IPC: C12P21/00 ; C12N5/00
Abstract:
Disclosed is an improved method for clarifying a cell culture during the manufacture of a protein. The method includes the step of transiently reducing the pH of a cell culture, followed by a holding step for a period of time, followed by the neutralization of the cell culture prior to clarification by centrifugation.
Information query
