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    USE OF TRIPLEX STRUCTURE DNA IN TRANSFERRING NUCLEOTIDE SEQUENCES
    3.
    发明申请
    USE OF TRIPLEX STRUCTURE DNA IN TRANSFERRING NUCLEOTIDE SEQUENCES 有权
    TRIPLEX结构DNA在转移核苷酸序列中的应用

    公开(公告)号：US20120095083A1

    公开(公告)日：2012-04-19

    申请号：US12813375

    申请日：2010-06-10

    申请人： Pierre Charneau ,  Véronique Zennou ,  Hüseyin Firat

    发明人： Pierre Charneau ,  Véronique Zennou ,  Hüseyin Firat

    IPC分类号： A61K31/7088 ,  A61P37/04 ,  A61P31/14 ,  C12N5/10

    CPC分类号： C12N15/87 ,  A01K2217/05 ,  A61K48/00 ,  A61K2039/51 ,  C07K14/4748 ,  C12N7/00 ,  C12N15/86 ,  C12N2740/16043 ,  C12N2740/16062 ,  C12N2830/50 ,  C12N2840/20 ,  C12N2840/203

    摘要： A method to induce an immune response in a host in need thereof, comprises administering to the host, recombinant lentiviral vector particles comprising: a) a GAG polypeptide or a functional GAG-polypeptide derivative; b) a POL polypeptide or a functional POL-polypeptide derivative ; c) an ENV polypeptide or a functional ENV-polypeptide derivative; and d) a recombinant polynucleotide. The recombinant polynucleotide comprises a transgene placed under the control of regulatory signals for transcription and expression, regulatory signals, of lentiviral origin, for reverse transcription, expression and packaging, and a polynucleotide comprising a cis-acting central initiation region (cPPT) and a cis-acting termination region (CTS). The regions are of lentiviral origin and are inserted in a functional orientation with the regulatory signals of lentiviral origin. The polynucleotide forms a DNA triplex during reverse transcription.

    摘要翻译： 在有需要的宿主中诱导免疫应答的方法包括向宿主施用重组慢病毒载体颗粒，其包含：a）GAG多肽或功能性GAG-多肽衍生物; b）POL多肽或功能性POL-多肽衍生物; c）ENV多肽或功能性ENV-多肽衍生物; 和d）重组多核苷酸。 重组多核苷酸包含置于转录和表达的调控信号控制下的转基因，慢病毒起源的调控信号，用于逆转录，表达和包装，以及包含顺式作用中枢起始区（cPPT）和顺式 作用终止区域（CTS）。 这些区域是慢病毒来源的，并且以具有慢病毒起源的调节信号的功能方向插入。 多核苷酸在逆转录过程中形成DNA三链体。

    Use of triplex structure DNA in transferring nucleotide sequences
    4.
    发明授权
    Use of triplex structure DNA in transferring nucleotide sequences 失效
    三重结构DNA在转移核苷酸序列中的应用

    公开(公告)号：US06682907B1

    公开(公告)日：2004-01-27

    申请号：US09688990

    申请日：2000-10-17

    申请人： Pierre Charneau ,  Véronique Zennou ,  Hüseyin Firat

    发明人： Pierre Charneau ,  Véronique Zennou ,  Hüseyin Firat

    IPC分类号： C12P2106

    CPC分类号： C12N15/87 ,  A01K2217/05 ,  A61K48/00 ,  A61K2039/51 ,  C07K14/4748 ,  C12N7/00 ,  C12N15/86 ,  C12N2740/16043 ,  C12N2740/16062 ,  C12N2830/50 ,  C12N2840/20 ,  C12N2840/203

    摘要： A recombinant vector comprising a polynucleotide containing a cis-acting central initiation region (cPPT) and a cis-acting termination region (CTS) is disclosed. These regions are of retroviral or retroviral-like origin. The vector further comprises a defined nucleotide sequence (transgene or sequence of interest) and regulatory signals for reverse transcription, expression, and packaging, wherein the regulatory signals are of retroviral or retroviral-like origin. In some embodiments the vector is incorporated into compositions for therapeutic purposes. In other embodiments the vector is incorporated into immunogenic compositions, The vector is also useful for methods such as ex vivo transfection or ex vivo transduction of non-mitotic differentiated cells.

    摘要翻译： 公开了包含含有顺式作用中心起始区（cPPT）和顺式作用终止区（CTS）的多核苷酸的重组载体。 这些区域是逆转录病毒或逆转录病毒样起源的。 载体还包含定义的核苷酸序列（转基因或感兴趣的序列）和用于逆转录，表达和包装的调节信号，其中调节信号是逆转录病毒或逆转录病毒样起源的。 在一些实施方案中，将载体并入用于治疗目的的组合物中。 在其它实施方案中，载体被并入免疫原性组合物中。载体也可用于非有丝分裂细胞分离细胞的离体转染或离体转导等方法。

    Lentiviral vectors for the preparation of immunotherapeutical compositions
    5.
    发明授权
    Lentiviral vectors for the preparation of immunotherapeutical compositions 有权
    用于制备免疫治疗组合物的慢病毒载体

    公开(公告)号：US08349606B2

    公开(公告)日：2013-01-08

    申请号：US13104380

    申请日：2011-05-10

    申请人： Pierre Charneau ,  Hüseyin Firat ,  Véronique Zennou

    发明人： Pierre Charneau ,  Hüseyin Firat ,  Véronique Zennou

    IPC分类号： C12N15/00 ,  A61K39/12 ,  C12Q1/70

    CPC分类号： C12N15/867 ,  A61K48/00 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  C07K14/35 ,  C12N15/86 ,  C12N2740/16043 ,  C12N2740/16134 ,  C12N2830/48 ,  C12N2830/50 ,  C12N2840/203

    摘要： The invention relates to an immunogenic composition comprising a recombinant vector characterized in that it comprises a polynucleotide comprising the cis-acting central initiation region (cPPT) and the cis-acting termination region (CTS), these regions being of retroviral or retroviral-like origin, said vector comprising in addition a defined nucleotide sequence (transgene or sequence of interest) and regulatory signals of retrotranscription, expression and encapsidation of retroviral or retroviral-like origin, wherein the composition is capable of inducing or of stimulating a cell-mediated response for instance a CTL (Cytotoxic T Lymphocytes) response or a CD4 response, against one or several epitopes encoded by the transgene sequence present in the vector.

    摘要翻译： 本发明涉及包含重组载体的免疫原性组合物，其特征在于其包含包含顺式作用中枢起始区（cPPT）和顺式作用终止区（CTS）的多核苷酸，这些区域是逆转录病毒或逆转录病毒样起源 所述载体另外包含定义的核苷酸序列（转基因或感兴趣的序列）以及逆转录病毒或逆转录病毒样起源的逆转录，表达和壳化的调控信号，其中所述组合物能够诱导或刺激细胞介导的应答 例如针对由载体中存在的转基因序列编码的一个或多个表位的CTL（细胞毒性T淋巴细胞）应答或CD4应答。

    Lentiviral vectors for the preparation of immunotherapeutical compositions
    7.
    发明授权
    Lentiviral vectors for the preparation of immunotherapeutical compositions 有权
    用于制备免疫治疗组合物的慢病毒载体

    公开(公告)号：US07968332B2

    公开(公告)日：2011-06-28

    申请号：US10313038

    申请日：2002-12-06

    申请人： Pierre Charneau ,  Hüseyin Firat ,  Véronique Zennou

    发明人： Pierre Charneau ,  Hüseyin Firat ,  Véronique Zennou

    IPC分类号： C12N15/00 ,  A61K39/12 ,  C12Q1/70

    CPC分类号： C12N15/867 ,  A61K48/00 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  C07K14/35 ,  C12N15/86 ,  C12N2740/16043 ,  C12N2740/16134 ,  C12N2830/48 ,  C12N2830/50 ,  C12N2840/203

    摘要： The invention relates to an immunogenic composition comprising a recombinant vector characterized in that it comprises a polynucleotide comprising the cis-acting central initiation region (cPPT) and the cis-acting termination region (CTS), these regions being of retroviral or retroviral-like origin, said vector comprising in addition a defined nucleotide sequence (transgene or sequence of interest) and regulatory signals of retrotranscription, expression and encapsidation of retroviral or retroviral-like origin, wherein the composition is capable of inducing or of stimulating a cell-mediated response for instance a CTL (Cytotoxic T Lymphocytes) response or a CD4 response, against one or several epitopes encoded by the transgene sequence present in the vector.

    摘要翻译： 本发明涉及包含重组载体的免疫原性组合物，其特征在于其包含包含顺式作用中枢起始区（cPPT）和顺式作用终止区（CTS）的多核苷酸，这些区域是逆转录病毒或逆转录病毒样起源 所述载体另外包含定义的核苷酸序列（转基因或感兴趣的序列）以及逆转录病毒或逆转录病毒样起源的逆转录，表达和壳化的调控信号，其中所述组合物能够诱导或刺激细胞介导的应答 例如针对由载体中存在的转基因序列编码的一个或多个表位的CTL（细胞毒性T淋巴细胞）应答或CD4应答。

    Use of triplex structure DNA in transferring nucleotide sequences
    8.
    发明授权
    Use of triplex structure DNA in transferring nucleotide sequences 有权
    三重结构DNA在转移核苷酸序列中的应用

    公开(公告)号：US08367068B2

    公开(公告)日：2013-02-05

    申请号：US12813375

    申请日：2010-06-10

    申请人： Pierre Charneau ,  Véronique Zennou ,  Hüseyin Firat

    发明人： Pierre Charneau ,  Véronique Zennou ,  Hüseyin Firat

    IPC分类号： A61K39/00 ,  A61K39/38 ,  A61K39/21 ,  A61K39/12

    CPC分类号： C12N15/87 ,  A01K2217/05 ,  A61K48/00 ,  A61K2039/51 ,  C07K14/4748 ,  C12N7/00 ,  C12N15/86 ,  C12N2740/16043 ,  C12N2740/16062 ,  C12N2830/50 ,  C12N2840/20 ,  C12N2840/203

    摘要： A method to induce an immune response in a host in need thereof, comprises administering to the host, recombinant lentiviral vector particles comprising: a) a GAG polypeptide or a functional GAG-polypeptide derivative; b) a POL polypeptide or a functional POL-polypeptide derivative ; c) an ENV polypeptide or a functional ENV-polypeptide derivative; and d) a recombinant polynucleotide. The recombinant polynucleotide comprises a transgene placed under the control of regulatory signals for transcription and expression, regulatory signals, of lentiviral origin, for reverse transcription, expression and packaging, and a polynucleotide comprising a cis-acting central initiation region (cPPT) and a cis-acting termination region (CTS). The regions are of lentiviral origin and are inserted in a functional orientation with the regulatory signals of lentiviral origin. The polynucleotide forms a DNA triplex during reverse transcription.

    摘要翻译： 在有需要的宿主中诱导免疫应答的方法包括向宿主施用重组慢病毒载体颗粒，其包含：a）GAG多肽或功能性GAG-多肽衍生物; b）POL多肽或功能性POL-多肽衍生物; c）ENV多肽或功能性ENV-多肽衍生物; 和d）重组多核苷酸。 重组多核苷酸包含置于转录和表达的调控信号控制下的转基因，慢病毒起源的调控信号，用于逆转录，表达和包装，以及包含顺式作用中枢起始区（cPPT）和顺式 作用终止区域（CTS）。 这些区域是慢病毒来源的，并且以具有慢病毒起源的调节信号的功能方向插入。 多核苷酸在逆转录过程中形成DNA三链体。