公开(公告)号：US11859204B2

公开(公告)日：2024-01-02

申请号：US16962851

申请日：2018-11-26

Applicant: PUSAN NATIONAL UNIVERSITY INDUSTRY-UNIVERSITY COOPERATION FOUNDATION

Inventor： Sik Yoon ,  Ye Seon Lim ,  Ye Jin Ok ,  Sun Young Hwang ,  Kang Oh Lee ,  Seung Soo Lee

IPC: B29C39/36 ,  C12N5/00 ,  B29C39/26

CPC classification number: C12N5/0062 ,  B29C39/265 ,  B29C39/36 ,  C12N5/0068 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2535/00

Abstract: A mold for producing a hydrogel support for a 3-dimensional (3D) cell culture, includes: an upper mold comprising a base and a plurality of upper unit molds protruding downward from the base to form accommodating portions of a female type corresponding to shapes of hydrogel supports, having through holes at bottoms thereof, and patterned at a lower portion of the base; a lower mold where a plurality of lower unit molds are patterned, the plurality of lower unit molds formed in a female type to respectively accommodate the plurality of upper unit molds protruding downward and having sealed lower portions; and an ejecting unit for separating, from the accommodating portions of the plurality of upper unit molds, the hydrogel supports that are coagulated after being inserted into the through holes respectively formed in the plurality of upper unit molds.

公开(公告)号：US11162068B2

公开(公告)日：2021-11-02

申请号：US16238537

申请日：2019-01-03

Applicant: PUSAN NATIONAL UNIVERSITY INDUSTRY-UNIVERSITY COOPERATION FOUNDATION

Inventor： Sik Yoon ,  Jong-Young Kwak ,  Song-Wan Jin ,  Young Hun Jeong ,  Chang Gun Kim ,  Da Jeong Choi ,  Hae Yeong Kang ,  Subhan Fazli ,  Muhamad Ikram

IPC: C12N5/00 ,  G01N33/543 ,  G01N33/50 ,  C12M1/12

Abstract: Disclosed is a method for manufacturing a composite nanofiber support and a fish collagen/synthetic polymer nanofiber support manufactured by the method, wherein the method comprises: a first step for dissolving a synthetic polymer in an organic solvent; a second step for dissolving a fish collagen in water to prepare an aqueous fish collagen solution; a third step for adding the aqueous fish collagen solution prepared in the second step to the synthetic polymer solution prepared in the first step, followed by mixing; and a fourth step for electrospinning the mixture solution prepared in the third step to manufacture a nanofiber support.

公开(公告)号：US10227560B2

公开(公告)日：2019-03-12

申请号：US15326638

申请日：2015-07-16

Applicant: PUSAN NATIONAL UNIVERSITY INDUSTRY-UNIVERSITY COOPERATION FOUNDATION

Inventor： Sik Yoon ,  Jong-Young Kwak ,  Song-Wan Jin ,  Young Hun Jeong ,  Chang Gun Kim ,  Da Jeong Choi ,  Hae Yeong Kang ,  Subhan Fazli ,  Muhamad Ikram

IPC: C12N5/00 ,  G01N33/50 ,  G01N33/543 ,  C12M1/12

Abstract: Disclosed is a method for manufacturing a composite nanofiber support and a fish collagen/synthetic polymer nanofiber support manufactured by the method, wherein the method comprises: a first step for dissolving a synthetic polymer in an organic solvent; a second step for dissolving a fish collagen in water to prepare an aqueous fish collagen solution; a third step for adding the aqueous fish collagen solution prepared in the second step to the synthetic polymer solution prepared in the first step, followed by mixing; and a fourth step for electrospinning the mixture solution prepared in the third step to manufacture a nanofiber support.