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公开(公告)号：US20240344035A1
公开(公告)日：2024-10-17
申请号：US18685054
申请日：2021-08-31
发明人： Qing Song LIU , Yu Ying HE , Cheng CHEN , Tao HUANG , Tao REN , Wen Chao WANG , Li WANG
IPC分类号： C12N5/09
CPC分类号： C12N5/0694 , C12N2500/32 , C12N2501/125 , C12N2501/22 , C12N2501/2303 , C12N2501/2306 , C12N2501/2307 , C12N2501/26 , C12N2503/02
摘要： Provided are a culture medium and a culture method for human primary acute myeloid leukemia cells. The culture medium for human primary acute myeloid leukemia cells contains a glutamine additive, non-essential amino acids, human interleukin-6, human interleukin-7, human interleukin-3, recombinant human FLT3 Ligand, a recombinant human macrophage colony stimulating factor and a human stem cell factor. Acute myeloid leukemia cells can be cultured with higher amplification efficiency and longer in-vitro culture time by using the culture medium and culture method. Also provided are human primary acute myeloid leukemia cells cultured in vitro by using the culture medium, and the use thereof for curative effect evaluation and screening of drugs.
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2.发明公开公开(公告)号：US20240344020A1
公开(公告)日：2024-10-17
申请号：US18577457
申请日：2021-07-28
发明人： Qingsong LIU , Jie HU , Tao HUANG , Cheng CHEN
CPC分类号： C12N5/0018 , C12N5/0693 , C12N2501/105 , C12N2501/117 , C12N2501/12 , C12N2501/2306 , C12N2501/999 , C12N2503/02
摘要： A primary cell culture medium for culturing primary lung cancer epithelial cells, containing an MST1/2 kinase inhibitor, a ROCK inhibitor, fibroblast growth factor 7, at least one additive of B27 additive and N2 additive, hepatocyte growth factor, insulin-like growth factor 1, interleukin 6, and a TGFβ type I receptor inhibitor. The invention also relates to a culture method using the primary cell culture medium and its application in the efficacy evaluating and screening of drugs. In the culture method, the primary cell culture medium is used to culture primary cells in a culture vessel coated with an extracellular matrix gel, to make the primary cells expend rapidly. A cell model obtained using the primary cell culture medium and the primary cell culture method can be used for the efficacy evaluating and screening of drugs.
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公开(公告)号：US20240319170A1
公开(公告)日：2024-09-26
申请号：US18275125
申请日：2021-02-05
发明人： Qingsong LIU , Yuying HE , Cheng CHEN , Tao HUANG , Tao REN , Wenchao WANG , Li WANG
CPC分类号： G01N33/5011 , C12N5/0693 , C12N2500/02 , C12N2501/01 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/117 , C12N2501/119 , C12N2501/12 , C12N2501/2322 , C12N2509/00
摘要： Disclosed are a culture medium and a culture method for in vitro expansion of primary cells of intestinal cancer. The culture medium comprises an initial culture medium, a Rho protease inhibitor, an antibiotic, gastrin, A8301, a non-essential amino acid, cholera toxin, an insulin-like growth factor-1, nicotinamide, insulin, fetal bovine serum, and an additive selected from at least one of a B27 additive and an N2 additive. By using the culture medium, effective and rapid expansion of the primary cells of intestinal cancer can be achieved. The expanded cells maintain the pathological characteristics of patients, and the culture success rate and the cell expansion rate of the primary cells of intestinal cancer are improved, which provide a research foundation for personalized treatment of the patients.
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