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公开(公告)号：US20220213540A1
公开(公告)日：2022-07-07
申请号：US17605640
申请日：2020-04-22
Inventor： Jeong Wook LEE , Gyoo Yeol JUNG , Chang Ha WOO , Sungho Jang , Giyoung SHIN
IPC: C12Q1/6862 , C12Q1/6876
Abstract: The present invention relates to a novel probe set for an isothermal one-pot reaction, and uses thereof and, particularly, provides a method for easily, accurately, and quickly diagnosing molecules, in particular, infection diseases, in the field, the method having a form applicable in the field on the basis of a nucleic acid sequence by using a one-pot reaction composition under an isothermal condition. If a molecular diagnostic platform according to the present invention is used, a target sequence can be quickly and accurately detected. Also, elements (reaction buffer, enzyme) needed in a diagnostic process using the present invention are much more simple and convenient than in a conventional antibody-based diagnosis, and, thus, the diagnostic process can be performed by a non-skilled person, and the sensitivity and speed of diagnosis can be increased, as all reactions are unified at a constant temperature without expensive equipment used in general nucleic-acid-based molecular diagnostic technology, and an amplification process is performed automatically during a reaction process.
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公开(公告)号：US20240376553A1
公开(公告)日：2024-11-14
申请号：US18658036
申请日：2024-05-08
Inventor： Jeong Wook LEE , Mi Bang KIM , Ye Rim SHIN , Chang Ha WOO
Abstract: The present disclosure relates to a probe set for isothermal one-pot reaction for detecting strains with biologically active biosynthetic pathway and uses thereof. The platform according to the present disclosure selectively binds only to the functional metabolite essential biosynthetic gene cluster by using the probe set according to the present disclosure, and thus it is possible to perform phenotype-based screening on strains isolated using the probe set according to the present disclosure, thereby effectively screening strains with biologically biosynthetic pathway (functional metabolite biosynthetic strains) in a fast and accurate manner. Furthermore, the platform according to the present disclosure is able to utilize RNA as well as DNA as targets, thereby detecting gene clusters that are actually actively expressed.
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公开(公告)号：US20250051829A1
公开(公告)日：2025-02-13
申请号：US18717794
申请日：2022-12-08
Inventor： Chang Ha WOO , Jeong Wook LEE , Jeongmin LEE , Doeon SUNG
IPC: C12Q1/6816 , C12Q1/6888
Abstract: The present invention relates to a method for generating a single-stranded region for detecting genetic mutation and a method for detecting genetic mutation using same. A detection method according to the present invention can detect sequence-specific nucleic acids and detect mutations at the single nucleotide polymorphism (SNP) discrimination level, thereby allowing for the accurate and sensitive discrimination of mutations in a short time. Thus, the present invention provides an effective mutation detection method that can respond not only to current SARS-CoV-2 variants but also generally to specific diseases or related mutations in the future.
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公开(公告)号：US20250092451A1
公开(公告)日：2025-03-20
申请号：US18967805
申请日：2024-12-04
Inventor： Jeong Wook LEE , Gyoo Yeol JUNG , Chang Ha WOO , Sungho Jang , Giyoung SHIN
IPC: C12Q1/6862 , C12Q1/6832 , C12Q1/6876
Abstract: The present invention relates to a novel probe set for an isothermal one-pot reaction, and uses thereof and, particularly, provides a method for easily, accurately, and quickly diagnosing molecules, in particular, infection diseases, in the field, the method having a form applicable in the field on the basis of a nucleic acid sequence by using a one-pot reaction composition under an isothermal condition. If a molecular diagnostic platform according to the present invention is used, a target sequence can be quickly and accurately detected. Also, elements (reaction buffer, enzyme) needed in a diagnostic process using the present invention are much more simple and convenient than in a conventional antibody-based diagnosis, and, thus, the diagnostic process can be performed by a non-skilled person, and the sensitivity and speed of diagnosis can be increased, as all reactions are unified at a constant temperature without expensive equipment used in general nucleic-acid-based molecular diagnostic technology, and an amplification process is performed automatically during a reaction process.
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