公开(公告)号：US20240376553A1

公开(公告)日：2024-11-14

申请号：US18658036

申请日：2024-05-08

Applicant: POSTECH Research and Business Development Foundation

Inventor： Jeong Wook LEE ,  Mi Bang KIM ,  Ye Rim SHIN ,  Chang Ha WOO

IPC: C12Q1/689 ,  C12Q1/682

Abstract: The present disclosure relates to a probe set for isothermal one-pot reaction for detecting strains with biologically active biosynthetic pathway and uses thereof. The platform according to the present disclosure selectively binds only to the functional metabolite essential biosynthetic gene cluster by using the probe set according to the present disclosure, and thus it is possible to perform phenotype-based screening on strains isolated using the probe set according to the present disclosure, thereby effectively screening strains with biologically biosynthetic pathway (functional metabolite biosynthetic strains) in a fast and accurate manner. Furthermore, the platform according to the present disclosure is able to utilize RNA as well as DNA as targets, thereby detecting gene clusters that are actually actively expressed.