公开(公告)号：US11352658B2

公开(公告)日：2022-06-07

申请号：US15500418

申请日：2015-07-31

申请人： Olink Bioscience AB

发明人： Ulf Landegren ,  Rachel Yuan Nong

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/00 ,  C07H21/04 ,  C12Q1/6813 ,  C12Q1/6844

摘要： The present invention relates to a method for selecting a target region of interest (ROI) in a target nucleic acid molecule using a nucleic acid probe comprising a 3′ sequence capable of hybridising to a target nucleic acid molecule and acting as a primer for the production of a complement of the target ROI (i.e. by target templated extension of the primer), and a sequence capable of templating the circularisation and ligation of the extended probe comprising the reverse complement of the target ROI and a portion of the probe. The circularised molecule thus obtained contains the reverse complement of the target ROI and may be subjected to further analysis and/or amplification etc. The probe may be provided as an oligonucleotide comprising a stem-loop structure or as a partially double-stranded construct and comprises a single-stranded 3′ end region containing the target-binding site. A second binding site provided in the probe serves as the ligation template for circularisation, and the stem-loop structure, if present, is cleaved to render the second binding site available for hybridisation to the target complement. Also provided are probes and kits for carrying out such a method.

公开(公告)号：US10597701B2

公开(公告)日：2020-03-24

申请号：US15374135

申请日：2016-12-09

申请人： Olink Bioscience AB

发明人： Ulf Landegren ,  Rachel Yuan Nong ,  Ola Söderberg ,  Irene Helbing

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6876

摘要： The present invention relates to a proximity-probe based detection assay for detecting an analyte in a sample and in particular to a method that comprises the use of at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte directly or indirectly, wherein the nucleic acid domain of at least one of said proximity probes comprises a hairpin structure that can be unfolded by cleavage of the nucleic acid domain to generate at least one ligatable free end or region of complementarity to another nucleic acid molecule in said sample, wherein when the probes bind to said analyte unfolding said hairpin structure allows the nucleic acid domains of said at least first and second proximity probes to interact directly or indirectly.

公开(公告)号：US20170211133A1

公开(公告)日：2017-07-27

申请号：US15374135

申请日：2016-12-09

申请人： Olink Bioscience AB

发明人： Ulf Landegren ,  Rachel Yuan Nong ,  Ola Söderberg ,  Irene Helbing

IPC分类号： C12Q1/68 ,  G01N33/542

CPC分类号： C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6876 ,  C12Q2521/301 ,  C12Q2525/301

摘要： The present invention relates to a proximity-probe based detection assay for detecting an analyte in a sample and in particular to a method that comprises the use of at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte directly or indirectly, wherein the nucleic acid domain of at least one of said proximity probes comprises a hairpin structure that can be unfolded by cleavage of the nucleic acid domain to generate at least one ligatable free end or region of complementarity to another nucleic acid molecule in said sample, wherein when the probes bind to said analyte unfolding said hairpin structure allows the nucleic acid domains of said at least first and second proximity probes to interact directly or indirectly.

公开(公告)号：US20170362643A9

公开(公告)日：2017-12-21

申请号：US15374135

申请日：2016-12-09

申请人： Olink Bioscience AB

发明人： Ulf Landegren ,  Rachel Yuan Nong ,  Ola Söderberg ,  Irene Helbing

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6876 ,  C12Q2521/301 ,  C12Q2525/301

摘要： The present invention relates to a proximity-probe based detection assay for detecting an analyte in a sample and in particular to a method that comprises the use of at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte directly or indirectly, wherein the nucleic acid domain of at least one of said proximity probes comprises a hairpin structure that can be unfolded by cleavage of the nucleic acid domain to generate at least one ligatable free end or region of complementarity to another nucleic acid molecule in said sample, wherein when the probes bind to said analyte unfolding said hairpin structure allows the nucleic acid domains of said at least first and second proximity probes to interact directly or indirectly.