公开(公告)号：US20120052581A1

公开(公告)日：2012-03-01

申请号：US13218076

申请日：2011-08-25

申请人： Michael Edward DeRosa ,  Hongwei Hanna Rao

发明人： Michael Edward DeRosa ,  Hongwei Hanna Rao

IPC分类号： C12N5/02 ,  B29C44/22

CPC分类号： C08J7/02 ,  C08J2325/06 ,  C08J2365/00

摘要： Surfaces of thermoplastic articles are rendered microporous by contacting the surface with a composition that includes a solvent. The article has a birefringence of 0.0001 or greater and the composition has a solvent strength configured to swell but not dissolve the polymer.

摘要翻译： 通过使表面与包含溶剂的组合物接触，使热塑性制品的表面变成微孔。 该制品具有0.0001以上的双折射性，并且该组合物具有溶胀强度但不溶解聚合物的溶剂强度。

公开(公告)号：US20120052565A1

公开(公告)日：2012-03-01

申请号：US13218103

申请日：2011-08-25

申请人： Michael Edward DeRosa ,  Joydeep Lahiri ,  Hongwei Hanna Rao ,  Po Ki Yuen

发明人： Michael Edward DeRosa ,  Joydeep Lahiri ,  Hongwei Hanna Rao ,  Po Ki Yuen

IPC分类号： C12M3/00 ,  C23F1/00

CPC分类号： C08J7/02 ,  C08J2323/08 ,  C08J2325/06 ,  C12M23/22 ,  C12M25/14

摘要： A cell culture article has substrate that is predominantly opaque and that provides a three-dimensional (e.g., irregular) surface, but incorporates an optically transparent, substantially regular (e.g., two-dimensional) surface to serve as a microscopic observation and imaging window for the 3D cell culture. In many embodiments, the 3D portion of the substrate occupies greater than 99% of the surface while the 2D portion occupies less than 1% of the surface so as not to substantially disrupt the general 3D culture environment.

摘要翻译： 细胞培养物具有主要是不透明的并且提供三维（例如，不规则）表面的基底，但是包含光学透明的，基本上规则的（例如，二维）表面，用作微观观察和成像窗口 3D细胞培养。 在许多实施例中，基板的3D部分占据表面的大于99％，而2D部分占据表面的1％以下，以免基本上破坏一般的3D文化环境。

公开(公告)号：US20110294142A1

公开(公告)日：2011-12-01

申请号：US12846209

申请日：2010-07-29

申请人： Joydeep Lahiri ,  Hongwei Hanna Rao ,  Jian Tan

发明人： Joydeep Lahiri ,  Hongwei Hanna Rao ,  Jian Tan

IPC分类号： G01N33/574 ,  B05D1/36 ,  G01N33/50

CPC分类号： C12N5/0068 ,  C12N5/0643 ,  C12N2533/18 ,  C12N2533/54 ,  G01N33/533

摘要： Described herein are multiply-fluorescently-labeled calcium phosphate protein substrates composed of a base coated with a fluorophore-labeled calcium phosphate coating and further having a fluorescently-labeled protein. The multiply-fluorescently-labeled substrate may be a fluorescently labeled calcium phosphate surface having a fluorescently-labeled collagen. The substrates are useful in culturing and studying the activity of a variety of cells, including bone cells. The substrates described herein can be used for both solution- and image-based analysis of cultured cells. New methods for producing and using such coated substrates are also disclosed.

摘要翻译： 本文描述的是多重荧光标记的磷酸钙蛋白质底物，其由涂布有荧光团标记的磷酸钙涂层的基底组成，并且还具有荧光标记的蛋白质。 多荧光标记的底物可以是具有荧光标记的胶原蛋白的荧光标记的磷酸钙表面。 底物可用于培养和研究包括骨细胞在内的各种细胞的活性。 本文所述的底物可用于培养细胞的基于溶液和图像的分析。 还公开了用于生产和使用这种涂覆的基材的新方法。

公开(公告)号：US08252549B2

公开(公告)日：2012-08-28

申请号：US12625952

申请日：2009-11-25

申请人： Hongwei Hanna Rao ,  Jian Tan

发明人： Hongwei Hanna Rao ,  Jian Tan

IPC分类号： C12Q1/04

CPC分类号： C12N5/0068 ,  C12M23/20 ,  C12M25/06 ,  C12N5/0643 ,  C12N2503/00 ,  C12N2533/18 ,  C12N2533/54 ,  G01N33/582

摘要： Described herein are multi-purpose substrates composed of (1) a base coated with a calcium phosphate coating and (2) a fluorophore-labeled collagen adsorbed on the calcium phosphate coating. The multi-purpose substrates are useful in culturing and studying the activity of a variety of cells. The multi-purpose substrates described herein can be used for both solution- and image-based analysis of cultured cells. New methods for producing and using such coated substrates are also disclosed.

摘要翻译： 本文描述的是由（1）涂覆有磷酸钙涂层的基底和（2）吸附在磷酸钙涂层上的荧光团标记的胶原构成的多功能底物。 多功能底物可用于培养和研究各种细胞的活性。 本文所述的多功能底物可用于培养细胞的溶液和图像分析。 还公开了用于生产和使用这种涂覆的基材的新方法。

公开(公告)号：US20110294151A1

公开(公告)日：2011-12-01

申请号：US12846043

申请日：2010-07-29

申请人： Hongwei Hanna Rao ,  Jian Tan

发明人： Hongwei Hanna Rao ,  Jian Tan

IPC分类号： C12Q1/02 ,  C07F9/572 ,  B32B27/32 ,  B05D3/06 ,  B32B17/06 ,  B32B9/04 ,  C12M1/34 ,  B05D3/02 ,  C07F9/09 ,  B32B27/36

CPC分类号： C12N5/0068 ,  C12N5/0643 ,  C12N2533/18 ,  G01N33/533 ,  G01N33/582 ,  Y10T428/31507 ,  Y10T428/31935 ,  Y10T428/31938

摘要： Described herein are substrates composed of a base coated with a fluorophore-labeled calcium phosphate coating. The substrates are useful in culturing and studying the activity of a variety of cells. The substrates described herein can be used for both solution- and image-based analysis of cultured cells. New methods for producing and using such coated substrates are also disclosed.

摘要翻译： 本文描述的是由涂布有荧光团标记的磷酸钙涂层的基底组成的底物。 底物可用于培养和研究各种细胞的活性。 本文所述的底物可用于培养细胞的基于溶液和图像的分析。 还公开了用于生产和使用这种涂覆的基材的新方法。

公开(公告)号：US20110124026A1

公开(公告)日：2011-05-26

申请号：US12625952

申请日：2009-11-25

申请人： Hongwei Hanna Rao ,  Jian Tan

发明人： Hongwei Hanna Rao ,  Jian Tan

IPC分类号： C12Q1/02 ,  C12N5/00

CPC分类号： C12N5/0068 ,  C12M23/20 ,  C12M25/06 ,  C12N5/0643 ,  C12N2503/00 ,  C12N2533/18 ,  C12N2533/54 ,  G01N33/582

摘要： Described herein are multi-purpose substrates composed of (1) a base coated with a calcium phosphate coating and (2) a fluorophore-labeled collagen adsorbed on the calcium phosphate coating. The multi-purpose substrates are useful in culturing and studying the activity of a variety of cells. The multi-purpose substrates described herein can be used for both solution- and image-based analysis of cultured cells. New methods for producing and using such coated substrates are also disclosed.

摘要翻译： 本文描述的是由（1）涂覆有磷酸钙涂层的基底和（2）吸附在磷酸钙涂层上的荧光团标记的胶原构成的多功能底物。 多功能底物可用于培养和研究各种细胞的活性。 本文所述的多功能底物可用于培养细胞的溶液和图像分析。 还公开了用于生产和使用这种涂覆的基材的新方法。