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1.发明申请Fractionation of Macro-Molecules Using Asymmetric Pulsed Field Electrophoresis 审中-公开使用不对称脉冲场电泳分离大分子公开(公告)号：US20090014332A1
公开(公告)日：2009-01-15
申请号：US12181827
申请日：2008-07-29
IPC分类号： B01D57/02
CPC分类号： C12N15/101 , Y10T436/10 , Y10T436/11 , Y10T436/25
摘要： A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.
摘要翻译： 提供了用于分离带电大分子如DNA的方法和装置。 将DNA溶液加载到包括障碍物阵列的矩阵中。 施加具有不同取向的两个不同场的交变电场。 交变电场是不对称的，因为一个场的持续时间或强度比另一个场强更强，否则是不对称的。 因此，DNA分子根据位点进行分级，并被驱动到基质的远侧，其中分离的DNA被回收。 分馏电场可用于负载和回收DNA以连续操作过程。
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2.发明申请METHOD FOR CONTINUOUS PARTICLE SEPARATION USING OBSTACLE ARRAYS ASYMMETRICALLY ALIGNED TO FIELDS 有权使用不对称地对齐的不正确的阵列的连续粒子分离方法公开(公告)号：US20120006728A1
公开(公告)日：2012-01-12
申请号：US13180823
申请日：2011-07-12
IPC分类号： B07B1/00
CPC分类号： B07B1/00 , B01L3/5027 , G01N27/44704 , G01N27/44773 , G01N27/44791 , G01N30/0005 , G01N30/02 , G01N30/6095 , B01D15/34
摘要： The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.
摘要翻译： 本发明涉及根据尺寸分离颗粒的方法和装置。 更具体地说，本发明涉及一种用于使用包括间隙网络的阵列根据尺寸分离颗粒的微流体方法和装置，其中来自每个间隙的场磁通不均匀地分成随后的间隙。 在一个实施例中，阵列包括在微流体通道中的有序阵列的障碍物，其中障碍物阵列相对于施加场的方向是不对称的。
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3.发明授权Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields 有权使用与场不对称的障碍物阵列进行连续粒子分离的方法公开(公告)号：US07150812B2
公开(公告)日：2006-12-19
申请号：US10693091
申请日：2003-10-23
IPC分类号： G01N27/447 , G01N27/453 , G01N30/02 , G01N15/06
CPC分类号： B07B1/00 , B01L3/5027 , G01N27/44704 , G01N27/44773 , G01N27/44791 , G01N30/0005 , G01N30/02 , G01N30/6095 , B01D15/34
摘要： The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.
摘要翻译： 本发明涉及根据尺寸分离颗粒的方法和装置。 更具体地说，本发明涉及一种用于使用包括间隙网络的阵列根据尺寸分离颗粒的微流体方法和装置，其中来自每个间隙的场磁通不均匀地分成随后的间隙。 在一个实施例中，阵列包括在微流体通道中的有序阵列的障碍物，其中障碍物阵列相对于施加场的方向是不对称的。
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4.发明授权Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields 有权使用与场不对称的障碍物阵列进行连续粒子分离的方法公开(公告)号：US08282799B2
公开(公告)日：2012-10-09
申请号：US13180823
申请日：2011-07-12
IPC分类号： G01N27/447 , G01N27/453 , G01N30/02 , G01N15/06
CPC分类号： B07B1/00 , B01L3/5027 , G01N27/44704 , G01N27/44773 , G01N27/44791 , G01N30/0005 , G01N30/02 , G01N30/6095 , B01D15/34
摘要： The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.
摘要翻译： 本发明涉及根据尺寸分离颗粒的方法和装置。 更具体地说，本发明涉及一种用于使用包括间隙网络的阵列根据尺寸分离颗粒的微流体方法和装置，其中来自每个间隙的场磁通不均匀地分成随后的间隙。 在一个实施例中，阵列包括在微流体通道中的有序阵列的障碍物，其中障碍物阵列相对于施加场的方向是不对称的。
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5.发明授权公开(公告)号：US06881317B2
公开(公告)日：2005-04-19
申请号：US10022189
申请日：2001-12-18
IPC分类号： C07H21/04 , B01L3/00 , C12N15/10 , G01N27/447 , C02F1/40 , C02F11/00 , C25B11/00 , G01N1/00 , G01N15/06
CPC分类号： C12N15/101 , Y10T436/10 , Y10T436/11 , Y10T436/25
摘要： A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.
摘要翻译： 提供了用于分离带电大分子如DNA的方法和装置。 将DNA溶液加载到包括障碍物阵列的矩阵中。 施加具有不同取向的两个不同场的交变电场。 交变电场是不对称的，因为一个场的持续时间或强度比另一个场强更强，否则是不对称的。 因此，DNA分子根据位点进行分级，并被驱动到基质的远侧，其中分离的DNA被回收。 分馏电场可用于负载和回收DNA以连续操作过程。
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6.发明授权Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields 有权使用与场不对称的障碍物阵列进行连续粒子分离的方法公开(公告)号：US07988840B2
公开(公告)日：2011-08-02
申请号：US11640111
申请日：2006-12-15
IPC分类号： G01N27/447 , G01N27/453 , G01N30/02 , G01N15/06
CPC分类号： B07B1/00 , B01L3/5027 , G01N27/44704 , G01N27/44773 , G01N27/44791 , G01N30/0005 , G01N30/02 , G01N30/6095 , B01D15/34
摘要： The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.
摘要翻译： 本发明涉及根据尺寸分离颗粒的方法和装置。 更具体地说，本发明涉及一种用于使用包括间隙网络的阵列根据尺寸分离颗粒的微流体方法和装置，其中来自每个间隙的场磁通不均匀地分成随后的间隙。 在一个实施例中，阵列包括在微流体通道中的有序阵列的障碍物，其中障碍物阵列相对于施加场的方向是不对称的。
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7.发明授权Method and apparatus for generating electric fields and flow distributions for rapidly separating molecules 有权用于产生用于快速分离分子的电场和流动分布的方法和装置公开(公告)号：US07597791B2
公开(公告)日：2009-10-06
申请号：US10147370
申请日：2002-05-15
IPC分类号： G01N27/453
CPC分类号： B01D57/02 , G01N27/44773
摘要： A method and apparatus for generating tunable, uniform electric fields in fluidic applications for rapid separation of molecules, such as DNA, is provided. A region receives the molecules to be separated, the molecules being injected into the region by an injection channel connected thereto. Fluidic microchannels or resistor arrays connected to sides of the region inject currents into the region and produce electric fields in the region that can be oriented at any angle. The electric fields can separate the molecules according to size, and can be used to move or manipulate the molecules within the region. Further, the molecules can be separated by controlling fluid flows within the region to manipulate the molecules. One or more reservoirs can be attached to the fluidic microchannels for collecting the molecules after separation, movement, or manipulation.
摘要翻译： 提供了一种用于在用于快速分离分子（例如DNA）的流体应用中产生可调谐均匀电场的方法和装置。 区域接收要分离的分子，分子通过与其连接的注入通道注入该区域。 连接到区域侧面的流体微通道或电阻器阵列将电流注入该区域，并在可以任意角度定向的区域中产生电场。 电场可以根据大小分离分子，并且可以用于移动或操纵该区域内的分子。 此外，可以通过控制区域内的流体流动来分离分子以操纵分子。 可以将一个或多个储存器连接到流体微通道，用于在分离，移动或操纵之后收集分子。
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