Invention Grant
- Patent Title: Fractionation of macro-molecules using asymmetric pulsed field electrophoresis
- Patent Title (中): 使用不对称脉冲场电泳分离大分子
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Application No.: US10022189Application Date: 2001-12-18
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Publication No.: US06881317B2Publication Date: 2005-04-19
- Inventor: Lotien Richard Huang , James Christopher Sturm , Robert Hamilton Austin
- Applicant: Lotien Richard Huang , James Christopher Sturm , Robert Hamilton Austin
- Applicant Address: US NJ Princeton
- Assignee: The Trustees of Princeton University
- Current Assignee: The Trustees of Princeton University
- Current Assignee Address: US NJ Princeton
- Agency: McCarter & English, LLP
- Main IPC: C07H21/04
- IPC: C07H21/04 ; B01L3/00 ; C12N15/10 ; G01N27/447 ; C02F1/40 ; C02F11/00 ; C25B11/00 ; G01N1/00 ; G01N15/06
Abstract:
A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.
Public/Granted literature
- US20020098504A1 Fractionation of macro-molecules using asymmetric pulsed field electrophoresis Public/Granted day:2002-07-25
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