公开(公告)号：US20140065624A1

公开(公告)日：2014-03-06

申请号：US13942437

申请日：2013-07-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kai LAO ,  Neil STRAUS

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/101 ,  C12Q2537/143 ,  C12Q2527/107 ,  C12Q2525/301 ,  C12Q2525/207 ,  C12Q2533/101

Abstract: The present teachings provide methods, compositions, and kits for performing primer extension reactions on at least two target polynucleotides in the same reaction mixture. In some embodiments, a reverse transcription reaction is performed on a first target polynucleotide with a hot start primer comprising a self-complementary stem and a loop, and extension products form at high temperatures but extension products form less so at low temperatures since the self-complementary stem of the hot start primer prevents hybridization of the target specific region to the target. However, non-hot start primers with free target specific regions can hybridize to their corresponding targets at the low temperature and extension can happen at the low temperature.

Abstract translation: 本教导提供用于在相同反应混合物中的至少两个靶多核苷酸上进行引物延伸反应的方法，组合物和试剂盒。 在一些实施方案中，对具有包含自身互补茎和环的热起始底物的第一靶多核苷酸进行逆转录反应，并且在高温下形成延伸产物，但延伸产物在低温下形成较少， 热启动引物的互补干扰物可以防止目标特异性区域与靶标的杂交。 然而，具有游离目标特异性区域的非热启动引物可以在低温下与其相应的靶标杂交，并且延伸可以在低温下发生。

公开(公告)号：US20170107567A1

公开(公告)日：2017-04-20

申请号：US15237266

申请日：2016-08-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kai LAO ,  Neil STRAUS ,  Nanlan XU

IPC: C12Q1/68

CPC classification number: C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q2600/158 ,  C12Q2525/301 ,  C12Q2525/207 ,  C12Q2525/161

Abstract: The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.

公开(公告)号：US20150322504A1

公开(公告)日：2015-11-12

申请号：US14723304

申请日：2015-05-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kai LAO ,  Neil STRAUS

IPC: C12Q1/68

CPC classification number: C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6876 ,  C12Q2549/119 ,  C12Q2525/301 ,  C12Q2525/155

Abstract: The present disclosure relates to the amplification of target nucleic acid sequences. This can be accomplished via the use of various primers. The use of these primers, as described herein, results in nucleic acid structures that can reduce the amplification of nonspecific hybridization events (such as primer dimerization) while allowing the amplification of the target nucleic acid sequences.