公开(公告)号：US20140248626A1

公开(公告)日：2014-09-04

申请号：US14192631

申请日：2014-02-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie LIU ,  Elena Bolchakova ,  Jaiprakash Shewale ,  Manohar Furtado

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6848 ,  C12Q2523/301 ,  C12Q2521/301 ,  C12Q2521/319

Abstract: In general, the disclosed method can be used to remove contaminating microbes and nucleic acids from microorganisms-derived reagents, apparatus and processes (materials and apparatus) related to PCR (and RT-PCR), including sample prep reagents and materials that are used to isolate, purify and detect nucleic acids.

Abstract translation: 通常，所公开的方法可用于从与来自微生物的试剂，与PCR（和RT-PCR）相关的装置和方法（材料和装置））中除去污染微生物和核酸，包括样品制备试剂和用于 分离，纯化和检测核酸。

公开(公告)号：US09988622B2

公开(公告)日：2018-06-05

申请号：US14697962

申请日：2015-04-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kevin Hacker ,  Gregory Govoni ,  Nikolay Sergeev ,  Elena Bolchakova ,  Maxim Brevnov ,  Manohar Furtado ,  Johnie Young ,  James Nurse ,  Mariela Cuadras

IPC: C12N15/10 ,  C12Q1/68 ,  G01N21/75 ,  C12N1/06 ,  B01D29/13

CPC classification number: C12N15/1003 ,  B01D29/13 ,  C12N1/066 ,  C12Q1/68 ,  C12Q1/689 ,  C12Q1/6895 ,  G01N21/75

Abstract: Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.