公开(公告)号：US10767217B2

公开(公告)日：2020-09-08

申请号：US15927572

申请日：2018-03-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole Bornarth ,  Michael Lau ,  Junko Stevens

IPC: C12Q1/68 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/6848 ,  C12Q1/686

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US11046727B2

公开(公告)日：2021-06-29

申请号：US15682103

申请日：2017-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tom Xu ,  Christopher Trinh ,  Carole Bornarth ,  Brian Evans ,  Mousumi Rath ,  Kathy Tran

IPC: C07H21/02 ,  C12Q1/6876 ,  C12N15/10 ,  C12Q1/6809 ,  C12Q1/6834

Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.

公开(公告)号：US10024863B2

公开(公告)日：2018-07-17

申请号：US14213204

申请日：2014-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole Bornarth ,  Mousumi Rath

IPC: G01N33/557 ,  G01N33/58 ,  G01N33/68 ,  C09B23/10 ,  C09B23/14

Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.

公开(公告)号：US11788126B2

公开(公告)日：2023-10-17

申请号：US16947727

申请日：2020-08-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole Bornarth ,  Michael Lau ,  Junko Stevens

IPC: C12Q1/6848 ,  C12N9/00 ,  C07K16/00 ,  C12Q1/686

CPC classification number: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686 ,  C12Q1/6848 ,  C12Q2527/125 ,  C12Q2547/107 ,  C12Q2549/101

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US20210147946A1

公开(公告)日：2021-05-20

申请号：US17091092

申请日：2020-11-06

Applicant: Life Technologies Corporation

Inventor： Charles Wendell Higdon, III ,  Harrison Leong ,  Charles Baudo ,  Carole Bornarth ,  Edgar Schreiber

IPC: C12Q1/6886 ,  C12Q1/6844 ,  G16B30/10 ,  G16B40/10

Abstract: Systems, primers, kits, and methods for detecting microsatellite instability in a biological sample are described. Signal data is received from a capillary electrophoresis genetic analysis instrument, wherein the signal data is measured from fluorescence of fragments comprising nucleic acid sequences amplified from the biological sample via polymerase chain reaction (PCR). The nucleic acid sequences correspond to a plurality of different microsatellite loci and are obtained using a plurality of PCR primers configured to flank a plurality of microsatellite loci of a biological sample. When the PCR primers and the biological sample are combined and subjected to PCR amplification, fluorescently labeled DNA fragments are generated comprising the plurality of microsatellite loci. Fluorescent data obtained from the plurality of fluorescently labelled microsatellite loci are used to classify microsatellite instability of the biological sample.

公开(公告)号：US09951378B2

公开(公告)日：2018-04-24

申请号：US13918768

申请日：2013-06-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole Bornarth ,  Michael Lau ,  Junko Stevens

IPC: C12Q1/68 ,  C12N9/00 ,  C07K16/00

CPC classification number: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2547/107 ,  C12Q2549/101

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.