公开(公告)号：US09388420B2

公开(公告)日：2016-07-12

申请号：US14600155

申请日：2015-01-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Anilkumar Kore ,  Shanmugasundaram Muthian ,  Kyle Gee

IPC: A01N43/04 ,  A61K31/70 ,  C12N15/67 ,  A61K31/7088 ,  A61K31/7084 ,  A61K31/7115 ,  A61K31/712 ,  C07C247/04 ,  C07D213/82 ,  C07H19/207 ,  C07H21/02 ,  C12Q1/68 ,  C12N15/11 ,  A61K48/00

CPC classification number: C12N15/67 ,  A61K31/7084 ,  A61K31/7088 ,  A61K31/7115 ,  A61K31/712 ,  A61K39/12 ,  A61K39/39 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/55511 ,  C07C247/04 ,  C07C323/60 ,  C07D213/82 ,  C07H1/00 ,  C07H1/02 ,  C07H1/04 ,  C07H19/207 ,  C07H21/02 ,  C12N7/00 ,  C12N15/11 ,  C12N2770/36134 ,  C12N2770/36171 ,  C12P19/34 ,  C12Q1/68

Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.