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    Sequencing Device
    2.
    发明申请
    Sequencing Device 有权
    排序设备

    公开(公告)号：US20150361488A1

    公开(公告)日：2015-12-17

    申请号：US14742404

    申请日：2015-06-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan SCHULTZ ,  Todd ROSWECH ,  Jon A. HOSHIZAKI ,  Albert L. CARRILLO ,  James A. BALL

    IPC: C12Q1/68 ,  G01N27/414

    CPC classification number: C12Q1/6869 ,  B01F1/0027 ,  B01F5/0683 ,  B01F11/0048 ,  B01F11/0071 ,  B01L3/502 ,  B01L3/505 ,  B01L3/527 ,  B01L3/563 ,  B01L2200/04 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/0681 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  C12Q1/6806 ,  G01N27/4145 ,  G01N35/1002 ,  G01N35/1095 ,  Y10T436/11 ,  Y10T436/143333

    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

    Abstract translation: 制备试剂的方法包括将药筒插入仪器中。 盒包括设置在盒的空腔中并将端口暴露于盒的外部的多个试剂盒。 每个试剂盒包括试剂容器，其包括试剂和限定可压缩体积的内腔，通过试剂容器限定到内腔的开口。 该方法还包括将多个流体端口连接到多个试剂盒的开口; 通过所述流体端口施加溶液以至少部分地填充所述多个试剂外壳; 并且循环空腔的压力，由此对于每个试剂外壳，在增加的压力期间，溶液进入试剂容器的内腔，与试剂结合，并压缩可压缩体积，并且在减压期间，可压缩体积 减少，试剂通过开口排出。

    SEQUENCING DEVICE
    3.
    发明申请
    SEQUENCING DEVICE 有权

    公开(公告)号：US20210130891A1

    公开(公告)日：2021-05-06

    申请号：US17146376

    申请日：2021-01-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan SCHULTZ ,  Todd ROSWECH ,  Jon A. HOSHIZAKI ,  Albert L. CARRILLO ,  James A. BALL

    IPC: C12Q1/6869 ,  G01N27/414 ,  C12Q1/6806 ,  B01F5/06 ,  B01F11/00 ,  B01L3/00 ,  B01F1/00 ,  G01N35/10

    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

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