公开(公告)号：US20240018520A1

公开(公告)日：2024-01-18

申请号：US18340838

申请日：2023-06-24

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup LEE ,  Dongsoo YANG ,  Jae Sung CHO

IPC: C12N15/113 ,  C12N15/63

CPC classification number: C12N15/113 ,  C12N2310/10 ,  C12N15/63

Abstract: Synthetic sRNA for inhibiting prokaryote gene expression is described, which includes (i) an Hfq binding site and (ii) a region that forms a complementary bond with a target gene mRNA. Vectors encoding the synthetic sRNA are described, as well as recombinant prokaryotes transformed with such vectors, methods of inhibiting prokaryote gene expression, and methods of gene screening and strain improvement. The synthetic sRNA is able to control single and multiple target genes at a time, and is particularly useful for inhibiting a gene expression of Gram-positive bacteria, e.g., in a recombinant Corynebacterium for mass production of high value products that do not require fossil fuels with associated environmental problems.

公开(公告)号：US20210054375A1

公开(公告)日：2021-02-25

申请号：US16960064

申请日：2019-03-08

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup LEE ,  Dongsoo YANG ,  Jae Sung CHO

IPC: C12N15/113 ,  C12N15/77 ,  C12Q1/689

Abstract: The present disclosure relate to a composition for inhibiting a prokaryotic expression and a use thereof and, more specifically, to a composition for inhibiting an expression of Gram-positive bacteria, which includes an sRNA comprising an sRNA-derived Hfq binding site from prokaryotes and (ii) a region that forms a complementary bond with a target gene mRNA and an Hfq from prokaryotes, a method of producing same, and a use thereof. A synthetic sRNA according to the present disclosure and a composition comprising the sRNA for inhibiting a gene expression having an advantage of being able to control single and multiple target genes at a time, can effectively reduce the expression of the target gene without the conventional gene deletion process via the synthetic sRNA that controls a gene expression so as to be useful for the production of a recombinant microorganism, and are particularly useful for inhibiting a gene expression of Gram-positive bacteria. A recombinant Corynebacterium produced by the present disclosure is a recombinant microorganism capable of mass production of high value products in an ecofriendly and reproducible manner on a bio-basis by controlling microbial metabolism flow through the synthetic sRNA. The recombinant microorganism, which is a bio-based production system developed through the sRNA is useful because of being able to replace existing fossil fuels while resolving environmental problems due to the ever-increasing use of crude oil.

公开(公告)号：US20240102068A1

公开(公告)日：2024-03-28

申请号：US18263127

申请日：2022-01-27

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup LEE ,  Dongsoo YANG ,  Woo Dae JANG

IPC: C12P19/44 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16

CPC classification number: C12P19/44 ,  C12N9/0004 ,  C12N9/1025 ,  C12N9/1288 ,  C12N9/16 ,  C12N9/93 ,  C12Y207/08007 ,  C12Y301/04052 ,  C12Y604/01002

Abstract: The present invention relates to novel C-glycosyltransferase variants and a use thereof. The C-glycosyltransferase variants according to the present invention have improved glycosidic bond-forming ability as compared with wild-type C-glycosyltransferase, and thus can increase the glycoside production effects of polyketide groups and pseudo-natural products, particularly type I, II, III polyketide, nonribosomal peptides, phenylpropanoids, and other aromatic natural products, and thus can be useful for the preparation of a drug, a food additive, a nutritional supplement, and the like containing a C-glycoside compound as a constituent ingredient.

公开(公告)号：US20210277428A1

公开(公告)日：2021-09-09

申请号：US16477897

申请日：2018-08-30

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Dongsoo YANG

IPC: C12P7/22 ,  C12N15/63 ,  C12N9/10 ,  C12P17/06 ,  C12P7/42 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/48

Abstract: The present invention relates to a recombinant microorganism for malonyl-CoA detection in which a type III polyketide synthase-encoding gene is inserted in the genome or in which a recombinant vector containing the gene is introduced; a method of screening a malonyl-CoA production-inducing substance using the recombinant microorganism; a method of screening a gene which is involved in increased malonyl-CoA production; and a method comprising knocking down the gene, screened by the method, in a microorganism, thus increasing the production of malonyl-CoA in the microorganism, and producing a useful substance in the microorganism using malonyl-CoA as a precursor. The use of the biosensor according to the present invention provides single-step signal generation, utilization in various microorganisms, utilization in self-fluorescent microorganisms, a simple construction method, and a simple screening method. In addition, when the present invention is combined with high-throughput screening, it has advantages in that strains having increased malonyl-CoA producing ability can be screened very easily and rapidly (˜3 days) and can be applied directly to the malonyl-CoA-based production of useful compounds.