公开(公告)号：US20210180102A1

公开(公告)日：2021-06-17

申请号：US17181230

申请日：2021-02-22

申请人： Kapa Biosystems, Inc.

发明人： William Bourn ,  Maryke Appel ,  Gavin Rush ,  John Foskett ,  Paul J. McEwan

IPC分类号： C12P19/34 ,  C12N9/12 ,  C12N15/10 ,  C12Q1/48

摘要： The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

公开(公告)号：US20200347441A1

公开(公告)日：2020-11-05

申请号：US16642813

申请日：2018-08-30

申请人： Kapa Biosystems, Inc.

发明人： Raeeza Allie ,  Michael Stuart Berry ,  William Bourn ,  Bjarne Faurholm ,  Suzanne Margaret Huddy ,  Martin Ranik ,  Du Toit Schabort

IPC分类号： C12Q1/6811 ,  C12N15/10 ,  C12Q1/6827

摘要： The disclosure provides compositions and methods for high-throughput screening a plurality of transposases to identify rare mutations that affect desired features of the transposase. Compositions of the disclosures are provided that facilitate the high-throughput screening methods of the disclosure.

公开(公告)号：US11932847B2

公开(公告)日：2024-03-19

申请号：US15491148

申请日：2017-04-19

申请人： Kapa Biosystems, Inc.

发明人： Martin Ranik ,  Eric Van der Walt ,  William Bourn ,  Jennifer Hsieh ,  Abre De Beer ,  Gerrida Uys ,  Paul McEwan

IPC分类号： C12N9/12 ,  C12N15/10 ,  C12Q1/6806

CPC分类号： C12N15/1065 ,  C12N9/1241 ,  C12Q1/6806 ,  C12Q1/6806 ,  C12Q2521/501 ,  C12Q2527/149

摘要： Disclosed is a method of fragmenting DNA comprising contacting a sample of target DNA with (a) a composition comprising an active transpososome, and (b) a composition comprising an inactive transpososome, under conditions suitable for transpososome activity, wherein a ratio of an amount of the inactive transpososome in the composition of (b) to an amount of the active transpososome in the composition of (a) determines the mean fragment size and a level of insertion bias.

公开(公告)号：US20170051326A1

公开(公告)日：2017-02-23

申请号：US15273670

申请日：2016-09-22

申请人： Kapa Biosystems, Inc.

发明人： William Bourn ,  Maryke Appel ,  Gavin Rush ,  John Foskett ,  Paul J. McEwan

IPC分类号： C12P19/34 ,  C12N15/10 ,  C12Q1/48 ,  C12N9/12

CPC分类号： C12P19/34 ,  C12N9/1241 ,  C12N9/1252 ,  C12N15/1058 ,  C12Q1/485 ,  C12Y207/07007 ,  G01N2333/9126

摘要： The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

摘要翻译： 本发明提供改进的DNA聚合酶，特别是A型DNA聚合酶，其可以更适合于重组DNA技术中的应用。 除此之外，本发明提供了衍生自定向进化实验的修饰的DNA聚合酶，设计用于选择在工业或研究应用中使用的条件下赋予有利表型的突变。

公开(公告)号：US10975361B2

公开(公告)日：2021-04-13

申请号：US15130339

申请日：2016-04-15

申请人： Kapa Biosystems, Inc.

发明人： Wolfgang Schafer ,  Paul McEwan ,  Eric Van Der Walt ,  John Foskett ,  William Bourn

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/686

摘要： The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

公开(公告)号：US09388396B2

公开(公告)日：2016-07-12

申请号：US14270304

申请日：2014-05-05

申请人： KAPA BIOSYSTEMS, INC.

发明人： Bjarne Faurholm ,  Paul McEwan ,  William Bourn ,  Gavin Rush

IPC分类号： C12N9/12 ,  C12P19/34

CPC分类号： C12N9/1252 ,  C12N9/1241 ,  C12P19/34

摘要： The present invention provides, among other things, chimeric DNA polymerases containing heterologous domains having sequences derived from at least two DNA polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, PCR additives (e.g., PCR enhancers) and other impurities; and high fidelity) of different DNA polymerases in a chimeric polymerase.

公开(公告)号：US11746339B2

公开(公告)日：2023-09-05

申请号：US17199343

申请日：2021-03-11

申请人： Kapa Biosystems, Inc.

发明人： Wolfgang Schafer ,  Paul J. McEwan ,  Eric van der Walt ,  John Foskett ,  William Bourn

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/686

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/686 ,  C12Y207/07007 ,  Y02P20/52

摘要： The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

公开(公告)号：US20190100783A1

公开(公告)日：2019-04-04

申请号：US15970769

申请日：2018-12-21

申请人： Kapa Biosystems, Inc.

发明人： William Bourn ,  Maryke Appel ,  Gavin Rush ,  John Foskett ,  Paul J. McEwan

IPC分类号： C12P19/34 ,  C12Q1/48 ,  C12N9/12 ,  C12N15/10

摘要： The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

公开(公告)号：US11603547B2

公开(公告)日：2023-03-14

申请号：US17181230

申请日：2021-02-22

申请人： Kapa Biosystems, Inc.

发明人： William Bourn ,  Maryke Appel ,  Gavin Rush ,  John Foskett ,  Paul J. McEwan

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12N15/10 ,  C12Q1/48

摘要： The present invention provides improved DNA polymerases, in particular, type A DNA polymerases, that may be better suited for applications in recombinant DNA technologies. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.

公开(公告)号：US20210317422A1

公开(公告)日：2021-10-14

申请号：US17199343

申请日：2021-03-11

申请人： Kapa Biosystems, Inc.

发明人： Wolfgang Schafer ,  Paul J. McEwan ,  Eric van der Walt ,  John Foskett ,  William Bourn

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/686

摘要： The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications.