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    Dot matrix membrane cell expansion and maintenance vessel
    4.
    发明授权
    Dot matrix membrane cell expansion and maintenance vessel 失效
    DOT MATRIX膜细胞扩增和维护血管

    公开(公告)号：US5068195A

    公开(公告)日：1991-11-26

    申请号：US266876

    申请日：1988-11-02

    申请人： Gary W. Howell ,  Joseph D. Irr ,  Ara T. Nahapetian

    发明人： Gary W. Howell ,  Joseph D. Irr ,  Ara T. Nahapetian

    IPC分类号： C12M1/00 ,  C12M3/06 ,  C12N5/02

    CPC分类号： C12M29/10 ,  C12M23/14 ,  C12M29/04

    摘要： There is disclosed a perfusable cell culture device capable of expansion and maintenance of biological cells, including mammalian, microbial, plant and insect cells in culture. The device comprises an upper and lower polymeric film layer and a gas and liquid permeable flow divider membrane. The flow divider membrane is affixed between the upper and lower polymeric layers and to the lower polymeric layer in a manner providing channels for fluid distribution of culture medium which is perfused at a controlled rate, uniformly across the flow divider membrane and to a bed of cells dispersed on the flow divider membrane.

    摘要翻译： 公开了能够扩增和维持生物细胞（包括哺乳动物，微生物，植物和昆虫细胞）的可灌注细胞培养装置。 该装置包括上和下聚合物膜层和气体和液体可渗透分流膜。 流动分隔膜以一定的方式固定在上下聚合物层和下聚合物层之间，从而提供用于流体分布的培养基的通道，培养基以受控的速率，均匀地流过分流器膜和细胞床 分散在分流膜上。

    Dot matrix membrane cell expansion
    5.
    发明授权
    Dot matrix membrane cell expansion 失效
    点阵膜细胞扩增

    公开(公告)号：US5139946A

    公开(公告)日：1992-08-18

    申请号：US725148

    申请日：1991-07-09

    申请人： Gary W. Howell ,  Joseph D. Irr ,  Ara T. Nahapetian

    发明人： Gary W. Howell ,  Joseph D. Irr ,  Ara T. Nahapetian

    IPC分类号： C12M1/00 ,  C12M3/06

    CPC分类号： C12M29/10 ,  C12M23/14 ,  C12M23/24 ,  C12M29/04 ,  Y10S435/818

    摘要： There is disclosed a perfusable cell culture device capable of expansion and maintenance of biological cells, including mammalian, microbial, plant and insect cells in culture. The device comprises an upper and lower polymeric film layer and a gas and liquid permeable flow divider membrane. The flow divider membrane is affixed between the upper and lower polymeric layers and to the lower polymeric layer in a manner providing channels for fluid distribution of culture medium which is perfused at a controlled rate, uniformly across the flow divider membrane and to a bed of cells dispersed on the flow divider membrane.

    摘要翻译： 公开了能够扩增和维持生物细胞（包括哺乳动物，微生物，植物和昆虫细胞）的可灌注细胞培养装置。 该装置包括上和下聚合物膜层和气体和液体可渗透的分流膜。 流动分隔膜以一定的方式固定在上下聚合物层和下聚合物层之间，从而提供用于流体分布的培养基的通道，培养基以受控的速率，均匀地流过分流器膜和细胞床 分散在分流膜上。