公开(公告)号：US20100099574A1

公开(公告)日：2010-04-22

申请号：US12644255

申请日：2009-12-22

Applicant: JOHN A. MOON ,  ALAN D. KERSEY ,  MARTIN A. PUTNAM ,  TUO LI

Inventor： JOHN A. MOON ,  ALAN D. KERSEY ,  MARTIN A. PUTNAM ,  TUO LI

IPC: C40B20/02

CPC classification number: G01N33/552 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/7743 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/54366 ,  G01N33/54386 ,  G03H1/0005 ,  G03H1/02 ,  G03H1/0236 ,  G03H1/26 ,  G03H2001/0033 ,  G03H2001/0044 ,  G03H2210/53 ,  G03H2210/63 ,  G03H2230/10 ,  G03H2250/12 ,  G06K19/04 ,  G06K19/06009

Abstract: A method of identifying an analyte. The method includes providing a plurality of microparticles. The microparticles have optically detectable codes extending along bodies of the corresponding microparticle. The microparticles have the chemical probes attached thereto. Each of the chemical probes is associated with a corresponding one of the codes. The method also includes selectively binding target analytes to the chemical probes on the microparticles to produce labeled microparticles and distributing the labeled microparticles to random locations of a substrate. The method also includes determining the codes for the labeled microparticles in the random array and code positions of the codes in the random array. The method further includes detecting the label on the labeled microparticles in the random array and label positions of the labels in the random array. The method also includes using the code positions and the label positions to analyze the target analyte.

Abstract translation: 识别分析物的方法。 该方法包括提供多个微粒。 微粒具有沿相应微粒的主体延伸的光学检测码。 微粒具有附着于其上的化学探针。 每个化学探针与相应的一个代码相关联。 该方法还包括将目标分析物选择性地结合到微粒上的化学探针以产生标记的微粒并将标记的微粒分布到基底的随机位置。 该方法还包括确定随机阵列中的标记微粒的代码和随机阵列中代码的代码位置。 该方法还包括随机阵列中标记的微粒上的标记和随机阵列中的标记的标记位置的检测。 该方法还包括使用代码位置和标签位置来分析目标分析物。

公开(公告)号：US20090194589A1

公开(公告)日：2009-08-06

申请号：US12174490

申请日：2008-07-16

Applicant: JOHN A. MOON ,  DAVID R. FOURNIER ,  MARTIN A. PUTNAM ,  ALAN D. KERSEY ,  TUO LI

Inventor： JOHN A. MOON ,  DAVID R. FOURNIER ,  MARTIN A. PUTNAM ,  ALAN D. KERSEY ,  TUO LI

IPC: G06K7/10

CPC classification number: G06K7/12 ,  G06K19/06084 ,  G06K19/06178 ,  G06K2019/06225

Abstract: An optical reader system that includes a plurality of substrates. The substrates have an optically readable code disposed therein and a source light assembly that is configured to illuminate the substrates with a code-reading beam and another beam for detecting another optically readable property of the substrate. The code-reading beam and the other beam form beam spots on the substrates that have different shapes. The system also includes a reader that is configured to receive output signals from the code-reading beam and the other beam when the substrates are illuminated. The output signals from the code-reading beam are indicative of the code.

Abstract translation: 一种包括多个基板的光学读取器系统。 基板具有设置在其中的光学可读代码，以及源光组件，其被配置为用代码读取光束照射基板，另一个光束用于检测基板的另一光学可读性。 代码读取光束和另一个光束在具有不同形状的基板上形成光束点。 该系统还包括读取器，其被配置为当基板被照亮时从代码读取光束和另一个光束接收输出信号。 代码读取波束的输出信号表示代码。

公开(公告)号：US20110114729A1

公开(公告)日：2011-05-19

申请号：US13014298

申请日：2011-01-26

Applicant: JOHN A. MOON ,  DAVID R. FOURNIER ,  MARTIN A. PUTNAM ,  ALAN D. KERSEY ,  TUO LI

Inventor： JOHN A. MOON ,  DAVID R. FOURNIER ,  MARTIN A. PUTNAM ,  ALAN D. KERSEY ,  TUO LI

IPC: G06K7/14

CPC classification number: G06K7/12 ,  G06K19/06084 ,  G06K19/06178 ,  G06K2019/06225

Abstract: An optical reader system including a source light assembly that has a code-reading beam and a fluorescence-excitation beam that are configured to illuminate encoded substrates. The substrates have optically readable codes that provide output signals when the code-reading beam is incident thereon. The output signals are indicative of the codes. The reader system also includes a fluorescence detector that is configured to detect fluorescent signals from the substrates and code pickup optics that are configured to project the output signals from the optically readable codes onto a Fourier plane. The reader system also includes a code detector that is positioned to detect the output signals in the Fourier plane.

Abstract translation: 一种光学读取器系统，包括具有代码读取光束的源光组件和被配置为照亮编码的基板的荧光激发光束。 衬底具有光学可读代码，当代码读取光束入射时，其提供输出信号。 输出信号表示代码。 读取器系统还包括荧光检测器，其被配置为检测来自基板的荧光信号和被配置为将来自光学可读代码的输出信号投影到傅立叶平面上的代码拾取光学器件。 读取器系统还包括代码检测器，其被定位成检测傅立叶平面中的输出信号。

公开(公告)号：US20110058172A1

公开(公告)日：2011-03-10

申请号：US12941568

申请日：2010-11-08

Applicant: JOHN A. MOON ,  ALAN D. KERSEY ,  MARTIN A. PUTNAM ,  TUO LI

Inventor： JOHN A. MOON ,  ALAN D. KERSEY ,  MARTIN A. PUTNAM ,  TUO LI

IPC: G01N21/25

CPC classification number: G01N33/552 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/7743 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/54366 ,  G01N33/54386 ,  G03H1/0005 ,  G03H1/02 ,  G03H1/0236 ,  G03H1/26 ,  G03H2001/0033 ,  G03H2001/0044 ,  G03H2210/53 ,  G03H2210/63 ,  G03H2230/10 ,  G03H2250/12 ,  G06K19/04 ,  G06K19/06009

Abstract: A method of identifying analytes that react with probes on encoded particles. The method includes providing a support substrate that has a plurality of the particles randomly distributed on the support substrate. The particles have elongated bodies with codes that extend along the corresponding bodies. The codes identify probes that are attached to the corresponding bodies, wherein at least some of the probes include fluorescent labels from reactions with the analytes. The method also includes detecting fluorescent signals that are emitted from the fluorescent labels. The fluorescent signals emit from random spatial locations along the support substrate. The method also includes detecting the codes of the particles at the random spatial locations along the support substrate and analyzing the codes and the fluorescent signals to identify the analytes that react with the probes on the particles.

Abstract translation: 识别与编码粒子上的探针反应的分析物的方法。 该方法包括提供具有随机分布在支撑衬底上的多个颗粒的支撑衬底。 颗粒具有细长体，其具有沿相应体部延伸的代码。 代码识别附着于相应体的探针，其中至少一些探针包括与分析物反应的荧光标记。 该方法还包括检测从荧光标记物发射的荧光信号。 荧光信号沿着支撑衬底从随机空间位置发射。 该方法还包括沿着支撑衬底的随机空间位置检测颗粒的代码并分析代码和荧光信号以识别与颗粒上的探针反应的分析物。