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    Lincosamides universal monoclonal antibody hybridoma cell strain and application thereof
    2.
    发明授权
    Lincosamides universal monoclonal antibody hybridoma cell strain and application thereof 有权

    公开(公告)号：US10808041B2

    公开(公告)日：2020-10-20

    申请号：US16699048

    申请日：2019-11-28

    申请人： Jiangnan University

    发明人： Hua Kuang ,  Lingling Guo ,  Chuanlai Xu ,  Liguang Xu ,  Wei Ma ,  Liqiang Liu ,  Shanshan Song ,  Xiaoling Wu

    IPC分类号： A61K39/395 ,  C07K16/44 ,  G01N33/94

    摘要： The present disclosure discloses a lincosamides universal monoclonal antibody hybridoma cell strain and application thereof, and belongs to the technical field of food safety immunological detection. According to the present disclosure, a clindamycin chlorine-substituted derivative is used as a hapten, the hapten is coupled with bovine serum albumin (BSA) by an activated ester method to obtain an immunizing antigen, and after being uniformly mixed with a Freund's adjuvant, the immunizing antigen is subcutaneously injected to immunize BALB/c mice; clindamycin is coupled with ovalbumin (OVA) by a carbonyl diimidazole (CDI) method to be used as a coating antigen used for detecting mouse serums and a cell supernatant. The spleen cells of the immunized mice are fused with mouse myeloma cells by a PEG method, and screened by indirect ELISA and indirect competitive ELISA and subcloned three times to obtain a population-selective hybridoma cell strain. The cell strain provided by the present disclosure has relatively good inhibition on clindamycin, lincomycin and pirlimycin, and can meet the demand for lincosamides multi-residue immunoassay products on the market.

    Hybridoma cell line of secreting clarithromycin monoclonal antibodies and preparation method thereof
    3.
    发明授权
    Hybridoma cell line of secreting clarithromycin monoclonal antibodies and preparation method thereof 有权

    公开(公告)号：US10696748B2

    公开(公告)日：2020-06-30

    申请号：US16394982

    申请日：2019-04-25

    申请人： JIANGNAN UNIVERSITY

    发明人： Chuanlai Xu ,  Hua Kuang ,  Lu Zeng ,  Liguang Xu ,  Liqiang Liu ,  Shanshan Song ,  Xiaoling Wu ,  Yongming Hu

    IPC分类号： C07K16/44 ,  A61K31/7048 ,  C12N5/12 ,  C07K16/06

    摘要： A hybridoma cell line of secreting clarithromycin monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14696 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with clarithromycin complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three subclones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to clarithromycin (value of IC50 is 0.3 ng/ml), being suitable for detection of clarithromycin in food.

    Hybridoma cell line of secreting clarithromycin monoclonal antibodies and preparation method thereof
    4.
    发明授权
    Hybridoma cell line of secreting clarithromycin monoclonal antibodies and preparation method thereof 有权

    公开(公告)号：US10323100B1

    公开(公告)日：2019-06-18

    申请号：US16222792

    申请日：2018-12-17

    申请人： JIANGNAN UNIVERSITY

    发明人： Chuanlai Xu ,  Hua Kuang ,  Lu Zeng ,  Liguang Xu ,  Liqiang Liu ,  Shanshan Song ,  Xiaoling Wu ,  Yongming Hu

    IPC分类号： C07K16/44 ,  A61K31/7048 ,  C12N5/12 ,  C07K16/06

    摘要： A hybridoma cell line of secreting clarithromycin monoclonal antibodies with a preservation number of hybridoma cell line of CGMCC No. 14696 belongs to the field of food safety immunological detection. BALB/c mice are immunized through one time immunization with complete freund's adjuvant, three times of booster immunization with incomplete freund's adjuvant and one time of rush immunization with clarithromycin complete antigen without adjuvant; the spleen cells from BALB/C mice immunized with high potency and low value of IC50 are fused with murine myeloma cells; and then the hybridoma cell line is obtained through indirect competitive ELISA screening and three subclones. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to clarithromycin (value of IC50 is 0.3 ng/ml), being suitable for detection of clarithromycin in food.

    Hybridoma cell strain secreting nifursolol residue marker monoclonal antibody
    6.
    发明授权
    Hybridoma cell strain secreting nifursolol residue marker monoclonal antibody 有权

    公开(公告)号：US10882896B2

    公开(公告)日：2021-01-05

    申请号：US16934709

    申请日：2020-07-21

    申请人： JIANGNAN UNIVERSITY

    发明人： Hua Kuang ,  Chuanlai Xu ,  Aihong Wu ,  Liguang Xu ,  Wei Ma ,  Liqiang Liu ,  Xiaoling Wu ,  Shanshan Song ,  Yongming Hu

    IPC分类号： C07K16/06 ,  C07K16/12 ,  C07K16/44 ,  G01N33/12

    摘要： A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 μg/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.

    Hybridoma cell strain secreting nifursolol residue marker monoclonal antibody
    7.
    发明授权
    Hybridoma cell strain secreting nifursolol residue marker monoclonal antibody 有权

    公开(公告)号：US10759845B2

    公开(公告)日：2020-09-01

    申请号：US16221719

    申请日：2018-12-17

    申请人： JIANGNAN UNIVERSITY

    发明人： Hua Kuang ,  Chuanlai Xu ,  Aihong Wu ,  Liguang Xu ,  Wei Ma ,  Liqiang Liu ,  Xiaoling Wu ,  Shanshan Song ,  Yongming Hu

    IPC分类号： C07K16/06 ,  C07K16/12 ,  C07K16/44 ,  G01N33/12

    摘要： A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody with CGMCC No. 18525 is prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 μg/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.