公开(公告)号：US11376584B2

公开(公告)日：2022-07-05

申请号：US16400629

申请日：2019-05-01

Applicant: Illumina, Inc.

Inventor： Sz-Chin Lin ,  Jay Taylor ,  Minsoung Rhee ,  Jennifer Foley ,  Wesley Cox-Muranami ,  Cyril Delattre ,  Tarun Khurana ,  Paul Crivelli

IPC: G01N21/05 ,  G01N21/03 ,  B01L3/00

Abstract: In one example, a flow cell includes a plurality of inlet ports sized to receive a flow of reagent from one of a plurality of reagents into the flow cell. An outlet port of the flow cell is sized to pass each flow of reagent out of the flow cell. A flow channel of the flow cell is positioned between, and in fluid communication with, each inlet port and the outlet port. The flow channel includes a manifold section and a detection section. The manifold section has a plurality of manifold branches in fluid communication with a common line, wherein each branch is connected to one of each inlet port. The detection section is in fluid communication with the common line and the outlet port. The detection section is operable to perform a plurality of different chemical reactions between the plurality of reagents and analytes positioned in the detection section.

公开(公告)号：US11951477B2

公开(公告)日：2024-04-09

申请号：US17809604

申请日：2022-06-29

Applicant: Illumina, Inc.

Inventor： Sz-Chin Lin ,  Jay Taylor ,  Minsoung Rhee ,  Jennifer Foley ,  Wesley Cox-Muranami ,  Cyril Delattre ,  Tarun Khurana ,  Paul Crivelli

IPC: B01L3/00 ,  G01N21/05

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502738 ,  G01N21/05 ,  B01L2300/0861 ,  B01L2400/0622 ,  B01L2400/08

Abstract: An example method includes connecting a flow cell to an instrument. The flow cell includes a flow channel including a manifold section having a manifold section swept volume and a detection section having a detection section swept volume. A ratio of the detection section swept volume to manifold section swept volume is at least 10 to 1. A first reagent is pumped through the flow channel. A first chemical reaction is performed between the first reagent and analytes positioned in the detection section. A subsequent reagent is pumped through the flow channel to flush out the remaining reagent. A concentration of at least 99.95 percent of reagent positioned in the detection section is the subsequent reagent, after pumping a total volume of the subsequent reagent through the flow channel that is equal to or less than 2.5 times a total swept volume of the manifold section plus the detection section.