公开(公告)号：US20200277634A1

公开(公告)日：2020-09-03

申请号：US16656816

申请日：2019-10-18

申请人： INVISTA North America S.á.r.l.

发明人： Alex Van Eck CONRADIE ,  Adriana L. BOTES ,  Alexander Brett FOSTER ,  Changlin CHEN

IPC分类号： C12P7/40 ,  C12P7/04 ,  C12P7/46 ,  C12P7/64 ,  C12P13/00 ,  C12P7/42 ,  C12P7/50 ,  C07C47/21 ,  C07C59/147 ,  C08G69/10 ,  C08G69/26 ,  C12N9/02 ,  C12N9/10 ,  C12N9/80 ,  C12N9/88 ,  C12N9/90 ,  C12P7/24 ,  C12P17/18

摘要： This document describes biochemical pathways for producing pimeloyl-CoA using a polypeptide having the enzymatic activity of a hydroperoxide lyase to form non-3-enal and 9-oxononanoate from 9-hydroxyperoxyoctadec-10,12-dienoate. Non-3-enal and 9-oxononanoate can be enzymatically converted to pimeloyl-CoA or a salt thereof using one or more polypeptides having the activity of a dehydrogenase, a CoA ligase, an isomerase, a reductase, a thioesterase, a monooxygenase, a hydratase, and/or a thiolase. Pimeloyl-CoA can be enzymatically converted to pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, or 1,7-heptanediol, or corresponding salts thereof. This document also describes recombinant microorganisms producing pimeloyl-CoA, as well as pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, and 1,7-heptanediol, or corresponding salts thereof.