公开(公告)号：US20230042267A1

公开(公告)日：2023-02-09

申请号：US17855325

申请日：2022-06-30

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Jonathan Mark Boutell ,  Wayne N. George ,  Xiaoyu Ma ,  Xiaolin Wu ,  Weixian Xi

IPC分类号： C08G63/685 ,  C08G63/82 ,  C08G63/91

摘要： An example of a kit includes a flow cell, a primer fluid, and a cleaving fluid. The flow cell includes at least one surface functionalized with a polymeric hydrogel including azide functional groups or amine functional groups. The primer fluid includes a plurality of alkyne-containing primers, each alkyne-containing primer having an amino cleavable group attaching a primer sequence of the alkyne-containing primer to an alkyne-containing moiety of the alkyne-containing primer. The cleaving fluid includes a substance that is reactive with the amino cleavable group.

公开(公告)号：US20220289876A1

公开(公告)日：2022-09-15

申请号：US17686828

申请日：2022-03-04

申请人： ILLUMINA, INC.

发明人： Brian Mather ,  Weixian Xi

IPC分类号： C08F8/30 ,  C08F220/56

摘要： In some examples, a method of coupling oligonucleotides to a polymer is provided. Inactive moieties in a first region of a polymer may be selectively irradiated with light, while inactive moieties in a second region of the polymer are not irradiated, to generate first active moieties in the first region of the polymer. The first active moieties may be coupled to first oligonucleotides. The inactive moieties in the second region of the polymer may be irradiated with light to generate second active moieties in the second region of the polymer. The second active moieties may be coupled to second oligonucleotides.

公开(公告)号：US20240352510A1

公开(公告)日：2024-10-24

申请号：US18633966

申请日：2024-04-12

申请人： Illumina, Inc.

发明人： Mathieu Lessard-Viger ,  Rebecca Turk-MacLeod ,  Vanessa Montaño-Machado ,  Jeffrey Fisher ,  Rohit Subramanian ,  Krishnarjun Sarkar ,  Sahngki Hong ,  Weixian Xi ,  Brandon Wenning ,  Lewis Kraft ,  Wayne George ,  Brian Mather ,  Allison Meade ,  John Daly

IPC分类号： C12Q1/6848 ,  C12Q1/6855

CPC分类号： C12Q1/6848 ,  C12Q1/6855

摘要： In some examples, a structure is contacted with polynucleotides having a variety of lengths and each including first and second adapters. The structure includes a substrate including first and second regions spaced apart from one another by a gap of at least 100 nm, a first set of capture primers coupled to the first region of the substrate, and a second set of capture primers coupled to the second region of the substrate. The first adapter of the polynucleotide is hybridized to a capture primer of the first set of capture primers. Based on that polynucleotide being sufficiently long to bridge the gap, it is amplified using the first and second sets of capture primers. Based upon that polynucleotide being insufficiently long to bridge the gap, it is not amplified. Optionally, a wall may be disposed in the gap.

公开(公告)号：US20230016633A1

公开(公告)日：2023-01-19

申请号：US17838986

申请日：2022-06-13

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Xiaoyu Ma ,  Weixian Xi ,  Jeffrey S. Fisher ,  Fei Shen ,  Wayne N. George ,  Brian D. Mather

IPC分类号： C08K5/54 ,  C12Q1/6874 ,  B01J19/00

摘要： Embodiments of the present application relate to substrate comprising a surface-bound azido functionalized organosilane wherein the substrate is free or substantially free of a hydrogel or a hydrophilic polymer. Methods of preparing such substrate surface for sequencing applications are also disclosed.

公开(公告)号：US20240209419A1

公开(公告)日：2024-06-27

申请号：US18539988

申请日：2023-12-14

申请人： Illumina, Inc.

发明人： Naneki McCallum ,  Fei Shen ,  Xiaoyu Ma ,  Weixian Xi ,  Olivia Ghazinejad ,  Jeffrey Brodin ,  Jeff Fisher

IPC分类号： C12Q1/6818 ,  C12Q1/6874

CPC分类号： C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6851

摘要： Methods for on-flow cell selective capture and enrichment of clustered beads, general capture strategies on bead mobility on flow cell surfaces, sorting clustered and unclustered beads, and flow cell reusability for bead immobilization onto flow cells.

公开(公告)号：US20230416435A1

公开(公告)日：2023-12-28

申请号：US18330298

申请日：2023-06-06

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Gianluca Andrea Artioli ,  Timothy J.N. Beech ,  Mathieu Lessard-Viger ,  Rebecca Turk-MacLeod ,  Brian D. Mather ,  Weixian Xi ,  Xavier von Hatten

IPC分类号： C08F220/56 ,  C08J3/075 ,  C12Q1/6869

CPC分类号： C08F220/56 ,  C08J3/075 ,  C12Q1/6869 ,  C08J2333/26

摘要： A co-polymer includes a plurality of a first monomer including a terminal functional group that is to attach to at least two different primers; a plurality of a second monomer including a second functional group that is different from the terminal functional group, and that is selected from the group consisting of a phenyl group, methoxy propyl, glycosyl, vinyl pyrrolidone, and an imidazole group; and a plurality of a third monomer that is different from the first and second monomers. This co-polymer may be used in a flow cell, and may enhance the clustering efficiency and kinetics.

公开(公告)号：US20240345475A1

公开(公告)日：2024-10-17

申请号：US18633865

申请日：2024-04-12

申请人： Illumina, Inc.

发明人： Krishnarjun Sarkar ,  Sahngki Hong ,  Weixian Xi ,  Brandon Wenning ,  Lewis Kraft ,  Jeffrey Fisher ,  Wayne George ,  Brian Mather

IPC分类号： G03F7/00

CPC分类号： G03F7/0002

摘要： In some examples, a method includes disposing a first hydrogel within a first recess of a substrate and over a pillar. The substrate may include a second recess in which the pillar is disposed, and a wall separating the first recess from the second recess. While the first hydrogel is disposed within the first recess, the pillar may be removed. After removing the pillar, a second hydrogel may be disposed within the second recess. Also provided herein are nonlimiting examples of manners in which recesses, walls, and patterned hydrogels may be formed.

公开(公告)号：US20240210829A1

公开(公告)日：2024-06-27

申请号：US18520093

申请日：2023-11-27

申请人： ILLUMINA, INC.

发明人： Jeffrey S. Fisher ,  Sahngki Hong ,  Lewis J. Kraft ,  David Prescott ,  Brandon Wenning ,  Weixian Xi

IPC分类号： G03F7/039 ,  G03F7/038 ,  G03F7/11 ,  G03F7/20

CPC分类号： G03F7/039 ,  G03F7/038 ,  G03F7/11 ,  G03F7/2004

摘要： In an example method, a positive photoresist is deposited over a substrate that includes depressions separated by interstitial regions. The positive photoresist is exposed to ultraviolet light at an angle that is non-perpendicular, non-parallel, and offset from a surface plane of the depressions such that a first portion of the positive photoresist in each depression remains soluble and a second portion of the positive photoresist in each depression is rendered insoluble. The soluble portions of the positive photoresist are removed, which exposes a first substrate portion in each depression. A first functionalized layer is deposited over the first substrate portion in each depression. The insoluble portions of the positive photoresist are removed, which exposes a second substrate portion in each depression. The second functionalized layer is selectively deposited over the second substrate portion in each depression.

公开(公告)号：US20240200135A1

公开(公告)日：2024-06-20

申请号：US18538441

申请日：2023-12-13

申请人： Illumina, Inc.

发明人： Weixian Xi ,  Brian Mather ,  Claudia Battistella

IPC分类号： C12Q1/6874 ,  B01L3/00

CPC分类号： C12Q1/6874 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0654

摘要： Reusable flow cells for sequencing which exhibit signal intensity retention over numerous use cycles, the active surface of which contains reactive sulfur moieties for reversible primer binding, methods of using such flow cells, reagents therefor, and kits containing the same.