摘要:
A process for efficient expression of a foreign gene in a Bacillus host was developed by varying the length and nucleotide sequence in the spacer region between ribosomal binding sequence (RBS) and initiation codon (ATG) of the gene. Bacillus thuringiensis cry2Ac gene was selected as a model gene because it requires an upstream open reading frame designated as orf2 for its efficient expression and crystallization in a Bacillus host.
摘要:
A chimeric protein comprising a cytolytic δ-endotoxin portion and a heterologous polypeptide portion is provided. Nucleic acids encoding the chimeric protein are also provided. In some cases, the chimeric protein can be expressed in parasporal inclusions of Bacillus thuringiensis. In certain cases, the cytolytic δ-endotoxin portion can include Cyt1Aa, and the heterologous portion can include an insecticidal polypeptide. The chimeric protein can be expressed in a suitable B. thuringiensi cell and purified as parasporal inclusions. Also provided is a method of controlling insects by exposing an insect to a chimerical insecticidal protein.
摘要:
A polymer electrolyte including a polymer fiber having a nanoscale diameter, wherein the polymer fiber is fabricated by an electrospinning method and a solar cell device exhibiting high energy conversion efficiency using the same. The solid-state electrolyte comprising such nanosized polymer fiber does not need a sealing agent and further simplifies the entire process compared to a conventional dye-sensitized solar cell using liquid electrolytes. Specifically, the energy conversion efficiency of the present dye-sensitized solar cell is significantly superior to that of a dye-sensitized solar cell using a polymer film electrolyte fabricated by a spin coating method. Further, the present dye-sensitized solar cell device can be obtained by using a scattering layer and compensating the surface effect.
摘要:
An error management system for managing an error occurring in a client application includes: a client terminal which intercepts an event, and collects error information and a crash dump file when the client application is abnormally terminated, and transmits the collected error information and the crash dump file in a predetermined format via a network; and an error collecting server which receives the error information and the crash dump file from the client terminal, and stores the received error information and the crash dump file for each online service, the error collecting server being independent from an online service server that executes a server application providing a service in correspondence to the client application.
摘要:
The invention relates to the discovery that nucleic acid sequences comprising a BtI or BtII promoter, or a combination of a BtI and a BtII promoter, a bacterial STAB-SD sequence, and a sequence encoding proteins of the B. sphaericus (“Bs”) binary toxin and expressed in B. thuringiensis (“Bt”) or Bs cells results in production of Bs binary toxin at least 10 times that of untransformed Bs cells. The invention provides nucleic acid sequences, expression vectors, host cells, and methods of increasing the toxicity of an insecticidal bacterium by transforming the bacterium with a nucleic acid sequence of the invention. Further, the invention relates to the discovery that the Cyt1Aa1 protein of Bt subspecies israelensis (“Bti”) reverses resistance to Bs binary toxin in Bs-resistant mosquitoes. The invention provides Bs cells expressing Bti Cyt1Aa1 protein, and methods of reversing resistance to Bs binary toxin by co-administering the Cyt1Aa1 protein with Bs binary toxin.
摘要:
An error management system for managing an error occurring in a client application includes: a client terminal which intercepts an event, and collects error information and a crash dump file when the client application is abnormally terminated, and transmits the collected error information and the crash dump file in a predetermined format via a network; and an error collecting server which receives the error information and the crash dump file from the client terminal, and stores the received error information and the crash dump file for each online service, the error collecting server being independent from an online service server that executes a server application providing a service in correspondence to the client application.
摘要:
Disclosed is a game system and method using a twin gage. In the game method, a twin gage for performing the ball game is provided to a user. The twin gage includes first and second swing gages arranged on the basis of an impact zone for determining a movement direction of the ball, and movable first and second scroll bars are formed on the first and second swing gages, respectively. A batting point of the ball according to the batting intention of the user is determined, and then a position of the impact zone of the twin gage is varied according to the determined batting point. Then, when the user manipulates the twin gage, the ball is moved in a movement direction of the ball determined according to a positional relation between the first and second scroll bars, which move and then stop according to the manipulation of the twin gage, and the impact zone. With such a configuration, a degree of difficulty of manipulation of a swing is varied according to the batting intention of the user, thereby making ball games more interesting.
摘要:
A method and apparatus for calculating an observation probability includes a first operation unit that subtracts a mean of a first plurality of parameters of an input voice signal from a second parameter of an input voice signal, and multiplies the subtraction result to obtain a first output. The first output is squared and accumulated N times in a second operation unit to obtain a second output. A third operation unit subtracts a given weighted value from the second output to obtain a third output, and a comparator stores the third output for a comparator stores the third output in order to extract L outputs therefrom, and stores the L extracted outputs based on an order of magnitude of the extracted L outputs.
摘要:
A polymer electrolyte including a polymer fiber having a nanoscale diameter, wherein the polymer fiber is fabricated by an electrospinning method and a solar cell device exhibiting high energy conversion efficiency using the same. The solid-state electrolyte comprising such nanosized polymer fiber does not need a sealing agent and further simplifies the entire process compared to a conventional dye-sensitized solar cell using liquid electrolytes. Specifically, the energy conversion efficiency of the present dye-sensitized solar cell is significantly superior to that of a dye-sensitized solar cell using a polymer film electrolyte fabricated by a spin coating method. Further, the present dye-sensitized solar cell device can be obtained by using a scattering layer and compensating the surface effect.