公开(公告)号：US20150056656A1

公开(公告)日：2015-02-26

申请号：US14473017

申请日：2014-08-29

Applicant: Hoffmann-La Roche Inc.

Inventor： Adelbert Grossmann ,  Friederike Hesse ,  Erhard Kopetzki ,  Wilma Lau ,  Christian Schantz

IPC: C07K14/775

CPC classification number: C07K14/775 ,  C12N15/67

Abstract: Herein is reported a method for the recombinant production of a polypeptide, which comprises the dipeptide AR, characterized in that the method comprises the recovering of the polypeptide from the cells or the cultivation medium of a cultivation of a cell comprising a nucleic acid encoding the polypeptide and thereby producing the polypeptide, whereby the dipeptide AR comprised in the polypeptide is encoded by the oligonucleotide gca cgt, or the oligonucleotide gcg cgt, or the oligonucleotide gcc cgt.

Abstract translation: 本文报道了重组产生多肽的方法，其包含二肽AR，其特征在于该方法包括从培养包含编码多肽的核酸的细胞或培养基的培养基中回收多肽 从而产生多肽，由此多肽中包含的二肽AR由寡核苷酸gca cgt或寡核苷酸gcg cgt或寡核苷酸gcc cgt编码。

公开(公告)号：US11591383B2

公开(公告)日：2023-02-28

申请号：US16367766

申请日：2019-03-28

Applicant: Hoffmann-La Roche Inc.

Inventor： Erhard Kopetzki ,  Oliver Ploettner

IPC: C12N15/85 ,  C07K16/00

Abstract: Herein is reported a nucleic acid comprising in 5′ to 3′ direction i) a first nucleic acid fragment encoding a polypeptide of interest without an in frame translational stop codon, ii) a second nucleic acid fragment operably linked to said first nucleic acid fragment which is beginning with the 5′ splice donor site of an immunoglobulin heavy chain CH3 or CH4 domain and which is terminated by the 3′ splice acceptor site of the succeeding immunoglobulin heavy chain transmembrane domain exon M1 and which comprises in frame translational stop codon and a polyadenylation signal, and iii) a third nucleic acid fragment operably linked to said second nucleic acid encoding at least a fragment of a transmembrane domain, wherein the second nucleic acid fragment has at its 3′ terminus the nucleotide sequence CTACCACCCCCTTCCTGTCCAG (SEQ ID NO: 29) or TGACCACGCCAATCGTGTCCAG (SEQ ID NO: 14) or CTACCACGCCAATCGTGTCCAG (SEQ ID NO: 31).

公开(公告)号：US20170210785A1

公开(公告)日：2017-07-27

申请号：US15480533

申请日：2017-04-06

Applicant: Hoffmann-La Roche Inc.

Inventor： Simone Hoege ,  Erhard Kopetzki ,  Dominique Ostler ,  Stefan Seeber ,  Georg Tiefenthaler

IPC: C07K16/00 ,  C12N15/85 ,  C12N5/0781

Abstract: In the method as reported herein the isolation of nucleic acid segments encoding antibody variable domains and the insertion of the isolated nucleic acid segments in eukaryotic expression plasmids is performed without the intermediate isolation and analysis of clonal intermediate plasmids. Thus, in the method as reported herein the intermediate cloning, isolation and analysis of intermediate plasmids is not required, e.g. by analysis of isolated transformed E. coli cells.

公开(公告)号：US20160083735A1

公开(公告)日：2016-03-24

申请号：US14721061

申请日：2015-05-26

Applicant: Hoffmann-La Roche Inc.

Inventor： Christian Klein ,  Erhard Kopetzki

IPC: C12N15/63 ,  C07K14/435

CPC classification number: C12N15/63 ,  C07K14/43595 ,  C07K14/4702 ,  C12N15/85

Abstract: The current invention reports a promoter having the nucleic acid sequence of SEQ ID NO: 02, or SEQ ID NO: 03, or SEQ ID NO: 04, or SEQ ID NO: 06, which is a 5′ shortened SV40 promoter with reduced promoter strength especially useful for the limited expression of heterologous polypeptides or selectable markers.

Abstract translation: 本发明报告了具有SEQ ID NO：02或SEQ ID NO：03或SEQ ID NO：04或SEQ ID NO：06的核酸序列的启动子，其是具有降低的启动子的5'缩短的SV40启动子 对于异源多肽或选择性标记的有限表达特别有用的强度。

公开(公告)号：US20150064746A1

公开(公告)日：2015-03-05

申请号：US14473367

申请日：2014-08-29

Applicant: Hoffmann-La Roche Inc.

Inventor： Adelbert Grossmann ,  Friederike Hesse ,  Erhard Kopetzki ,  Wilma Lau ,  Christian Schantz

IPC: C07K14/775 ,  C07K14/47

CPC classification number: C07K14/775 ,  C07K14/4726 ,  C07K2319/00 ,  C12N15/67

Abstract: Herein is reported a method for the recombinant production of a polypeptide, which comprises the tripeptide QKK, characterized in that the method comprises the step of recovering the polypeptide from the cells or the cultivation medium of a cultivation of a cell comprising a nucleic acid encoding the polypeptide and thereby producing the polypeptide, whereby the tripeptide QKK comprised in the polypeptide is encoded by the oligonucleotide cag aaa aaa or the oligonucleotide caa aag aaa.

Abstract translation: 本文报道了重组产生多肽的方法，其包含三肽QKK，其特征在于所述方法包括从细胞或培养基中培养多肽的步骤，所述培养基包含编码 多肽，从而产生多肽，由此多肽中包含的三肽QKK由寡核苷酸cag aaa aaa或寡核苷酸caa aag aaa编码。

公开(公告)号：US08541204B2

公开(公告)日：2013-09-24

申请号：US13705339

申请日：2012-12-05

Applicant: Hoffmann-La Roche Inc.

Inventor： Josef Endl ,  Erhard Kopetzki ,  Oliver Ploettner ,  Ursula Schwarz ,  Georg Tiefenthaler

IPC: C12P21/04

CPC classification number: C12N15/85 ,  C07K16/00 ,  C07K16/065 ,  C07K2319/03

Abstract: The current invention describes a nucleic acid comprising in a 5′ to 3′ direction a) a first nucleic acid encoding a heterologous polypeptide without an in frame stop codon, b) a second nucleic acid beginning with a 5′ splice donor site and terminated by a 3′ splice acceptor site comprising an in frame translational stop codon and a polyadenylation signal, and c) a nucleic acid encoding i) at least a fragment of a transmembrane domain, or ii) a signal peptide for a GPI-anchor.

Abstract translation: 本发明描述了包含在5'至3'方向的核酸a）编码不具有​​帧内终止密码子的异源多肽的第一核酸，b）以5'剪接供体位点开始并以 3''剪接受体位点，其包含内部翻译终止密码子和多聚腺苷酸化信号，以及c）编码i）至少跨膜结构域片段的核酸，或ii）GPI锚的信号肽。

公开(公告)号：US20130109058A1

公开(公告)日：2013-05-02

申请号：US13705339

申请日：2012-12-05

Applicant: Hoffmann-La Roche Inc.

Inventor： Josef Endl ,  Erhard Kopetzki ,  Oliver Ploettner ,  Ursula Schwarz ,  Georg Tiefenthaler

IPC: C12N15/85

CPC classification number: C12N15/85 ,  C07K16/00 ,  C07K16/065 ,  C07K2319/03

Abstract: The current invention describes a nucleic acid comprising in a 5′ to 3′ direction a) a first nucleic acid encoding a heterologous polypeptide without an in frame stop codon, b) a second nucleic acid beginning with a 5′ splice donor site and terminated by a 3′ splice acceptor site comprising an in frame translational stop codon and a polyadenylation signal, and c) a nucleic acid encoding i) at least a fragment of a transmembrane domain, or ii) a signal peptide for a GPI-anchor.

公开(公告)号：US12024548B2

公开(公告)日：2024-07-02

申请号：US16244909

申请日：2019-01-10

Applicant: Hoffmann-La Roche Inc.

Inventor： Simone Dethloff ,  Erhard Kopetzki ,  Dominique Ostler ,  Stefan Seeber ,  Georg Tiefenthaler

IPC: C12N15/85 ,  C07K16/00 ,  C12N5/0781 ,  C12N15/10 ,  C12P21/02

CPC classification number: C07K16/00 ,  C12N5/0635 ,  C12N15/1086 ,  C12N15/85 ,  C12P21/02 ,  C07K2317/14 ,  C07K2317/56 ,  C12N2510/02 ,  C12N15/1086 ,  C12Q2521/507 ,  C12Q2537/143

Abstract: In the method as reported herein the isolation of nucleic acid segments encoding antibody variable domains and the insertion of the isolated nucleic acid segments in eukaryotic expression plasmids is performed without the intermediate isolation and analysis of clonal intermediate plasmids. Thus, in the method as reported herein the intermediate cloning, isolation and analysis of intermediate plasmids is not required, e.g. by analysis of isolated transformed E. coli cells.

公开(公告)号：US11407836B2

公开(公告)日：2022-08-09

申请号：US16133579

申请日：2018-09-17

Applicant: Hoffmann-La Roche Inc.

Inventor： Sebastian Fenn ,  Erhard Kopetzki ,  Georg Tiefenthaler

IPC: C07K16/28 ,  C12N9/52 ,  C07K16/46 ,  C07K16/32 ,  C07K16/40

Abstract: Herein is reported a method for producing a bispecific antibody comprising the step of incubating (i) an antibody Fab fragment or a scFv antibody comprising within the 20 C-terminal amino acid residues the amino acid sequence LPX1TG (SEQ ID NO: 01), (ii) a one-armed antibody comprising a full length antibody heavy chain, a full length antibody light chain, and an Fc-heavy chain, whereby the full length antibody heavy chain and the full length antibody light chain are cognate antibody chains that thereof forms an antigen binding site, whereby the full length antibody heavy chain and the Fc-heavy chain are covalently linked to each other via one or more disulfide bonds forming an antibody hinge region, and whereby the Fc-heavy chain has an oligoglycine amino acid sequence at its N-terminus, and (iii) a Sortase A enzyme.

公开(公告)号：US11169146B2

公开(公告)日：2021-11-09

申请号：US15625950

申请日：2017-06-16

Applicant: Hoffmann-La Roche Inc.

Inventor： Mara Boenitz-Dulat ,  Erhard Kopetzki ,  Peter Kratzsch ,  Martin Schatte

IPC: G01N33/53 ,  C12Q1/37 ,  C12Q1/00 ,  A61K38/00

Abstract: Herein is reported a method for the detection of a sortase in a sample, comprising the following steps: a) incubating the sample with a first substrate comprising an immobilization tag and a second substrate comprising a detectable label, whereby in the presence of a sortase in the sample a conjugate comprising the immobilization tag and the detectable label is formed, b) immobilizing the conjugate of step a) via/using the immobilization tag to a solid phase, c) detecting the immobilized conjugate via/using the detectable label and thereby detecting the sortase in the sample.