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公开(公告)号：US20130260434A1
公开(公告)日：2013-10-03
申请号：US13806491
申请日：2011-06-21
IPC分类号： C12N13/00
摘要： The invention relates to an electrode assembly 20, in particular for applying at least one electric field to adherent cells, comprising at least two electrodes 21, each having at least one surface 32 which is arranged opposite the corresponding surface 32 of the other electrode 21, wherein an electrically insulating material 26 is arranged at least partially between the surfaces 32 of the electrodes 21. The solution according to the invention allows the electric field to be concentrated in the region of the cells to be treated such that a voltage pulse, or the current produced thereby, flows through the cells without the majority flowing away over the cells unused in the electrolyte. The invention further relates to a method for applying at least one electric field to adherent cells, in which the electric field is generated by applying a voltage to at least two electrodes, the electric field is concentrated on the side of the electrodes which faces the cells and/or is limited to the space between the cells and the side of the electrodes which faces the cells.
摘要翻译： 本发明涉及电极组件20，特别是用于将至少一个电场施加到贴壁电池，包括至少两个电极21，每个具有至少一个与另一电极21的对应表面32相对的表面32， 其中电绝缘材料26至少部分地布置在电极21的表面32之间。根据本发明的溶液允许电场集中在要处理的电池的区域中，使得电压脉冲或 由此产生的电流流过电池，而绝大多数电解液不会在电解质中未使用的电池上流动。 本发明还涉及一种用于将至少一个电场施加到贴壁电池的方法，其中通过向至少两个电极施加电压而产生电场，电场集中在面对电池的电极侧 和/或限于电池与面对电池的电极侧之间的空间。
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公开(公告)号：US20130230895A1
公开(公告)日：2013-09-05
申请号：US13806395
申请日：2011-06-21
IPC分类号： C12N13/00
摘要： The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. The invention further relates to a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.
摘要翻译： 本发明涉及一种使贴壁细胞经受至少一个电场的方法，其中通过向至少两个有效电极63施加电压而产生电场，其中至少提供三个电极63,64，其中在 当施加电压以产生第一电场时，至少两个电极63,64是有效电极63，并且其中产生至少一个第二电场，其中两个先前有效电极63中的至少一个是电位 - 当施加电压时，电极64。 本发明还涉及包括至少三个电极63,64的装置58，这些电极通过至少一个开关装置59,60,61,62连接到至少一个电压源，其中至少五个电极63,64 通过四个开关装置59,60,61,62连接到至少一个电压源，其中至少两个电极63,64通过公共开关装置59,60,61连接到电压源， 结合上述方法，根据本发明的装置允许使用电场对粘附细胞进行有效和均匀的处理，其中有效和无电极之间的开关可以使用较低的 开关器件数量。
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公开(公告)号：US09701954B2
公开(公告)日：2017-07-11
申请号：US13806395
申请日：2011-06-21
摘要： The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. The invention further relates to a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.
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公开(公告)号：US09624486B2
公开(公告)日：2017-04-18
申请号：US13806491
申请日：2011-06-21
摘要： The invention relates to an electrode assembly 20, in particular for applying at least one electric field to adherent cells, comprising at least two electrodes 21, each having at least one surface 32 which is arranged opposite the corresponding surface 32 of the other electrode 21, wherein an electrically insulating material 26 is arranged at least partially between the surfaces 32 of the electrodes 21. The solution according to the invention allows the electric field to be concentrated in the region of the cells to be treated such that a voltage pulse, or the current produced thereby, flows through the cells without the majority flowing away over the cells unused in the electrolyte. The invention further relates to a method for applying at least one electric field to adherent cells, in which the electric field is generated by applying a voltage to at least two electrodes, the electric field is concentrated on the side of the electrodes which faces the cells and/or is limited to the space between the cells and the side of the electrodes which faces the cells.
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公开(公告)号：US09080139B2
公开(公告)日：2015-07-14
申请号：US13145124
申请日：2010-01-20
摘要： The invention relates to a container (1) with at least three reaction spaces (2) which in each case have at least one electrode pair for applying an electric voltage for generating an electric field within the reaction space (2) and which are arranged geometrically in at least one row and/or electrically connected in at least one row, wherein at least one electrode (3, 4) of a reaction space (2) is a common electrode (3, 4) with at least one other reaction space (2). According to the invention n+x electrodes (3, 4, 5) are provided, wherein n is the number of reaction spaces (2), with n≧3 and x is the number of rows, with x≧1.
摘要翻译： 本发明涉及具有至少三个反应空间（2）的容器（1），每个反应空间（2）在每种情况下具有至少一个电极对，用于施加用于在反应空间（2）内产生电场的电压并且几何地布置 在至少一排中和/或电连接在至少一排中，其中反应空间（2）的至少一个电极（3,4）是具有至少一个其它反应空间的公共电极（3,4） 2）。 根据本发明，提供n + x电极（3,4,5），其中n是反应空间（2）的数量，n≥3，x是行数，x≥1。
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公开(公告)号：US20120087841A1
公开(公告)日：2012-04-12
申请号：US13145124
申请日：2010-01-20
摘要： The invention relates to a container (1) with at least three reaction spaces (2) which in each case have at least one electrode pair for applying an electric voltage for generating an electric field within the reaction space (2) and which are arranged geometrically in at least one row and/or electrically connected in at least one row, wherein at least one electrode (3, 4) of a reaction space (2) is a common electrode (3, 4) with at least one other reaction space (2). According to the invention n+x electrodes (3, 4, 5) are provided, wherein n is the number of reaction spaces (2), with n≧3 and x is the number of rows, with x≧1.
摘要翻译： 本发明涉及具有至少三个反应空间（2）的容器（1），每个反应空间（2）在每种情况下具有至少一个电极对，用于施加用于在反应空间（2）内产生电场的电压并且几何地布置 在至少一排中和/或电连接在至少一排中，其中反应空间（2）的至少一个电极（3,4）是具有至少一个其它反应空间的公共电极（3,4） 2）。 根据本发明，提供n + x电极（3,4,5），其中n是反应空间（2）的数量，n≥3，x是行数，x≥1。
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7.发明授权公开(公告)号：US09284525B2
公开(公告)日：2016-03-15
申请号：US13145142
申请日：2010-01-20
CPC分类号： C12M35/02 , C12M23/44 , Y10T29/49117
摘要： The invention relates to a method for the application of at least one voltage pulse to at least two containers fitted with electrodes, by means of which at least one voltage pulse is applied to at least one container, while at least one other container is subjected to a preparation or a post-processing. According to the invention the method comprises mutual exchange of the respective positions of the container to which a voltage pulse has already been applied and the further container. Furthermore, the invention relates to a device (1) for making electrical contact with at least one container fitted with electrodes, with at least one receptacle (3, 7) upon which at least one container can be set, and with at least one contact appliance (8) for making contact with the electrodes of the container. According to the invention at least two receptacles (3, 7) are provided, wherein one receptacle (7) is located at or inside the contact appliance (8), and wherein both receptacles (3, 7) and/or the contact appliance (8) can be moved such that after completion of the movement the other receptacle (3) is located at or inside the contact appliance (8).
摘要翻译： 本发明涉及一种用于将至少一个电压脉冲应用于装配有电极的至少两个容器的方法，通过该电容器将至少一个电压脉冲施加到至少一个容器，同时至少一个其它容器经受 准备或后期处理。 根据本发明，该方法包括相互更换已经施加了电压脉冲的容器的相应位置和另外的容器。 此外，本发明涉及一种用于与装有电极的至少一个容器进行电接触的装置（1），其中至少一个容器（3,7）可以在其上设置至少一个容器，并具有至少一个触点 器具（8），用于与容器的电极接触。 根据本发明，提供了至少两个插座（3,7），其中一个插座（7）位于接触器具（8）中或其内部，并且其中两个插座（3,7）和/或触头装置 8）可以移动，使得在移动完成之后，另一容器（3）位于联系设备（8）中或内部。
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公开(公告)号：US09249384B2
公开(公告)日：2016-02-02
申请号：US13145113
申请日：2010-01-20
IPC分类号： C12M1/42
CPC分类号： C12M35/02
摘要： The invention relates to a method for applying at least one electrical voltage pulse to at least one reaction space by at least partially discharging at least one charge storing unit for storing electrical charges. According to the invention the method comprises applying at least one voltage pulse to a reaction space by at least partially discharging a second charge storing unit and simultaneously charging or partially discharging a first charge storing. Furthermore, the invention relates to a device 1 for applying at least one electrical voltage pulse to at least one reaction space, comprising at least two charge storing units (10, 11) for storing electrical charges and at least one power supply unit (12) for charging the charge storing units (10, 11) and/or at least one discharge device for partially discharging the charge storing units (10, 11). According to the invention it is provided that at least two of the charge storing units (10, 11) form a common storage module which can be discharged at any time.
摘要翻译： 本发明涉及通过至少部分地排放至少一个用于存储电荷的电荷存储单元来将至少一个电压脉冲施加到至少一个反应空间的方法。 根据本发明，该方法包括通过至少部分地排放第二电荷存储单元并同时对第一电荷存储进行充电或部分放电来将至少一个电压脉冲施加到反应空间。 此外，本发明涉及一种用于向至少一个反应空间施加至少一个电压脉冲的装置1，包括用于存储电荷的至少两个电荷存储单元（10,11）和至少一个电源单元（12） 用于对电荷存储单元（10,11）充电和/或用于部分地放电电荷存储单元（10,11）的至少一个放电装置。 根据本发明，提供至少两个电荷存储单元（10,11）形成可在任何时间放电的公共存储模块。
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公开(公告)号：US08450102B2
公开(公告)日：2013-05-28
申请号：US12035960
申请日：2008-02-22
申请人： Andreas Wirth , Michael Kazinski
发明人： Andreas Wirth , Michael Kazinski
CPC分类号： G01N27/44756 , B01L3/5027 , C12M35/02
摘要： A device 1 with at least one electrode 8 for generating an electrical field in a chamber 7 is disclosed. The device 1 comprises at least one input channel 6 for introducing a fluid into the chamber 7, and at least one output channel 11 for discharging the fluid from the chamber 7. Also disclosed is a method for stabilizing the flow of a fluid through a chamber 7 in which an electrical field is generated and which has at least one input channel 6 for introducing the fluid into the chamber and at least one output channel 11 for discharging the fluid from the chamber 8. To avoid undesirable backflow of the fluid due to gas formation, the average inside diameter of the input channel 6 of device 1 is smaller than the average inside diameter of the output channel 11.
摘要翻译： 公开了一种具有至少一个用于在腔室7中产生电场的电极8的装置1。 装置1包括用于将流体引入室7的至少一个输入通道6和用于从腔室7排出流体的至少一个输出通道11.还公开了一种用于使流体通过腔室 7，其中产生电场并且具有用于将流体引入腔室的至少一个输入通道6和用于从腔室8排出流体的至少一个输出通道11.为了避免由于气体而导致的流体的不期望的回流 装置1的输入通道6的平均内径小于输出通道11的平均内径。
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公开(公告)号：US20120214708A1
公开(公告)日：2012-08-23
申请号：US13334319
申请日：2011-12-22
申请人： Richard Wagner , Karsten Gall , Andreas Wirth
发明人： Richard Wagner , Karsten Gall , Andreas Wirth
CPC分类号： G01N33/48728 , G01N33/5005
摘要： The invention relates to a measuring device for measuring at least one property of cells or vesicles, characterized in that is divided into two chambers by a hydrophobic partition having a horizontal opening, these chambers containing electrolyte solution and said opening being closed by a lipid double layer. The invention is characterized in that at least one cell or vesicle contacts the lipid double layer. The invention also relates to methods involving the use of the measuring device and to arrays, measuring chambers and microtitration plates all suited therefor.
摘要翻译： 本发明涉及一种用于测量细胞或囊泡的至少一种性质的测量装置，其特征在于：通过具有水平开口的疏水隔板将其分为两个腔室，这些腔室包含电解质溶液，所述开口由脂质双层 。 本发明的特征在于，至少一个细胞或囊泡接触脂质双层。 本发明还涉及涉及使用测量装置和全部适用于其的阵列，测量室和微量滴定板的方法。
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