公开(公告)号：US20170330724A1

公开(公告)日：2017-11-16

申请号：US15527562

申请日：2015-11-26

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Taiga OKUMURA ,  Takashi OHSHIMA ,  Yuusuke OOMINAMI ,  Minami SHOUJI ,  Akiko HISADA ,  Akio YONEYAMA

IPC: H01J37/28 ,  H01J37/20

CPC classification number: H01J37/28 ,  G01N2223/418 ,  H01J37/20 ,  H01J37/22 ,  H01J37/222 ,  H01J2237/0245 ,  H01J2237/20 ,  H01J2237/20207 ,  H01J2237/20214 ,  H01J2237/228 ,  H01J2237/2802

Abstract: An electronic microscope has a great depth of focus compared with an optical microscope. Thus, information is superimposed in the depth direction in one image. Thus, observation of a three-dimensional structure inside a specimen with use of the electronic microscope requires accurate specification of a three-dimensional position or density of a structure inside the specimen. Furthermore, the specimen on a slide glass that is observed with the optical microscope may not be put in a TEM device in the related art. Thus, a very complicated preparation of the specimen is required for performing three-dimensional internal structure observation, with the electronic microscope, of a location that is observed with the optical microscope.Provided is a charged particle beam device including a charged particle optical column that irradiates a specimen with a primary charged particle beam, and a specimen base rotating unit that is capable of rotating the specimen base in a state of an angle formed by a surface of the specimen base and an optical axis of the primary charged particle beam being inclined to a non-perpendicular angle, in which the specimen base is configured to include a detecting element that detects a charged particle scattered or transmitted inside the specimen, and transmitted charged particle images of the specimen corresponding to each angle is acquired by irradiating the specimen in a state of the specimen base rotating unit being rotated at a plurality of different angles.

公开(公告)号：US20190013177A1

公开(公告)日：2019-01-10

申请号：US15753685

申请日：2015-08-21

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Yusuke OMINAMI ,  Akiko HISADA

IPC: H01J37/16 ,  H01J37/28 ,  H01J37/20 ,  H01J37/244

CPC classification number: H01J37/16 ,  H01J37/20 ,  H01J37/244 ,  H01J37/28 ,  H01J2237/1825 ,  H01J2237/2003 ,  H01J2237/2007

Abstract: In order to observe a water-containing sample with excellent convenience under an air atmosphere or a gas atmosphere, or under a desired pressure, in the present invention, there is provided an observation support unit for observation by irradiating the sample disposed in a non-vacuum space separated by a diaphragm from an inner space of a charged particle optical lens barrel that generates a charged particle beam, with the charged particle beam. The observation support unit includes a main body portion for covering a hole portion that forms an observation region where the sample is observed, and the sample, and the observation support unit is directly mounted between the sample and the diaphragm, that is, on the sample.

公开(公告)号：US20160168525A1

公开(公告)日：2016-06-16

申请号：US14891155

申请日：2014-05-09

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Akiko HISADA ,  Hiroshi SONODA

IPC: C12M1/12 ,  C12N5/071

CPC classification number: C12M25/16 ,  C12N5/0671 ,  C12N2533/54

Abstract: Provided is a culturing method capable of causing a three-dimensional aggregate in which cell function is similar to in vivo function to adhere to a culturing vessel while improving permeability of nutrients, oxygen, drugs and reagents from the culture medium and maintaining cell function. By sowing isolated cells in a culturing vessel, and after the cells have settled on the culturing surface, administering microscaffold particles that are cell-adhesive and substance-permeable onto the cells, it is possible to cause the cells to adhere to the culturing vessel while forming cell aggregates that encompass the particles. For example, cell aggregates encompassing the particles are formed by administering gelatin particles in which the size of the culture carrier to be encompassed in the cell aggregate is at least ⅕ of the cell diameter at a cell number:particle number of at least 80:1 with respect to the number of cultured cells per unit culturing area.

Abstract translation: 本发明提供一种培养方法，其能够使细胞功能与体内功能相似的三维聚集体粘附于培养容器，同时提高营养物，氧，药物和试剂从培养基中的渗透性，并维持细胞功能。 通过在培养容器中播种分离的细胞，并且在细胞沉积在培养表面上之后，将细胞粘附和物质可渗透的微支架颗粒施用到细胞上，可以使细胞粘附到培养容器上，同时 形成包含颗粒的细胞聚集体。 例如，包含颗粒的细胞聚集体通过施用明胶颗粒而形成，其中包含在细胞集合体中的培养载体的大小至少为细胞数目的细胞直径的⅕：粒子数至少为80:1 相对于每单位培养面积的培养细胞数。