公开(公告)号：US20080031813A1

公开(公告)日：2008-02-07

申请号：US11872139

申请日：2007-10-15

申请人： Gary Griffiths ,  Hans Hansen ,  David Goldenberg

发明人： Gary Griffiths ,  Hans Hansen ,  David Goldenberg

IPC分类号： A61K31/69 ,  A61K31/70 ,  A61K38/00 ,  A61K51/00 ,  A61P43/00

CPC分类号： A61K51/109 ,  A61K31/70 ,  A61K38/02 ,  A61K38/465 ,  A61K47/645 ,  A61K47/665 ,  A61K47/6853 ,  A61K47/6893 ,  A61K47/6898 ,  A61K51/0497 ,  A61K51/065 ,  B82Y5/00 ,  C07K16/4266

摘要： The present invention is directed to methods for treating cancer wherein more than one therapeutic agent is used, with each of the therapeutic agents having different tumor-killing capabilities, and wherein the therapeutic agents are delivered to the tumor sites using combined targeting and pre-targeting methods. The methods of the present invention achieve good tumor to non-tumor ratios of the therapeutic agents, and are effective for cancer therapy.

摘要翻译： 本发明涉及用于治疗癌症的方法，其中使用多于一种治疗剂，每种治疗剂具有不同的杀伤能力，并且其中使用组合靶向和预靶向将治疗剂递送至肿瘤部位 方法。 本发明的方法实现了治疗剂的非肿瘤比例的良好肿瘤，并且对于癌症治疗是有效的。

公开(公告)号：US20060002855A1

公开(公告)日：2006-01-05

申请号：US11210684

申请日：2005-08-25

申请人： Gary Griffiths ,  Hans Hansen ,  David Goldenberg

发明人： Gary Griffiths ,  Hans Hansen ,  David Goldenberg

IPC分类号： A61K51/00 ,  A61K38/19 ,  C07K16/46 ,  A61K36/37 ,  A61K36/185

CPC分类号： A61K33/22 ,  A61K31/7088 ,  A61K33/24 ,  A61K38/465 ,  A61K45/06 ,  A61K47/645 ,  A61K47/665 ,  A61K47/6853 ,  A61K47/6893 ,  A61K47/6898 ,  A61K51/0497 ,  A61K51/065 ,  A61K51/10 ,  A61K51/1096 ,  B82Y5/00 ,  Y02A50/463 ,  A61K2300/00

摘要： The present invention is directed to methods for treating cancer wherein more than one therapeutic agent is used, with each of the therapeutic agents having different tumor-killing capabilities, and wherein the therapeutic agents are delivered to the tumor sites using combined targeting and pre-targeting methods. The methods of the present invention achieve good tumor to non-tumor ratios of the therapeutic agents, and are effective for cancer therapy.

公开(公告)号：US20070048227A1

公开(公告)日：2007-03-01

申请号：US11553814

申请日：2006-10-27

申请人： Gary Griffiths ,  Serengulam Govindan ,  Hans Hansen

发明人： Gary Griffiths ,  Serengulam Govindan ,  Hans Hansen

IPC分类号： A61K39/00

CPC分类号： C07K16/44 ,  A61K47/665 ,  A61K47/67 ,  B82Y5/00 ,  C07K16/3007 ,  C07K2317/31 ,  C07K2317/55

摘要： Embodiments of the invention relate to a method for treating target cells, tissues or pathogens in a subject, comprising administering a non-covalently bound complex which comprises a multispecific targeting protein and a hapten-enzyme covalent conjugate, followed by administration of a prodrug that is converted to an active drug by the enzyme. Other embodiments of the invention further relate to kits comprising the non-covalently bound complex or the components to prepare the complex.

摘要翻译： 本发明的实施方案涉及用于治疗受试者中靶细胞，组织或病原体的方法，包括施用包含多特异性靶向蛋白质和半抗原酶共价缀合物的非共价结合复合物，然后施用前药，其为 通过酶转化为活性药物。 本发明的其它实施方案还涉及包含非共价结合复合物或制备复合物的组分的试剂盒。

公开(公告)号：US20060104899A1

公开(公告)日：2006-05-18

申请号：US11216033

申请日：2005-09-01

申请人： Hans Hansen ,  Gary Griffiths ,  Shui-on Leung ,  William McBride ,  Zhengxing Qu

发明人： Hans Hansen ,  Gary Griffiths ,  Shui-on Leung ,  William McBride ,  Zhengxing Qu

IPC分类号： A61K51/00 ,  A61K39/395 ,  A61K49/16

CPC分类号： C07K16/468 ,  A61K47/6863 ,  A61K47/6899 ,  A61K51/1048 ,  A61K51/1063 ,  A61K2039/505 ,  B82Y5/00 ,  C07K16/18 ,  C07K16/30 ,  C07K16/3007 ,  C07K16/3046 ,  C07K16/44 ,  C07K2317/12 ,  C07K2317/13 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2319/00

摘要： The present invention relates to a bi-specific antibody or antibody fragment having at least one arm that is reactive against a targeted tissue and at least one other arm that is reactive against a linker moiety. The linker moiety encompasses a hapten to which antibodies have been prepared. The antigenic linker is conjugated to one or more therapeutic or diagnostic agents or enzymes. The invention provides constructs and methods for producing the bispecific antibodies or antibody fragments, as well as methods for using them.

公开(公告)号：US06808874B2

公开(公告)日：2004-10-26

申请号：US09877919

申请日：2001-06-07

申请人： Gary Griffiths

发明人： Gary Griffiths

IPC分类号： C12Q100

CPC分类号： G01N33/573

摘要： We describe a method for monitoring the activity of an enzyme, the method comprising the steps of: providing a binding domain which includes a site for enzymatic modification; providing a binding partner which binds to the binding domain in a manner which is dependent upon modification of the site. The binding domain is contacted with the enzyme; and binding of the binding domain to the binding partner is detected as an indication of the activity of the enzyme. One of the binding domain and binding partner comprises a polypeptide and the other of the binding domain and binding partner comprises a nucleic acid.

摘要翻译： 我们描述了一种用于监测酶的活性的方法，该方法包括以下步骤：提供包含酶修饰位点的结合结构域; 提供以取决于位点修饰的方式结合结合域的结合配偶体。 结合结构域与酶接触; 检测结合结构域与结合配偶体的结合作为酶的活性的指示。 结合结构域和结合配偶体之一包含多肽，另一个结合结构域和结合配偶体包含核酸。

公开(公告)号：US20050271671A1

公开(公告)日：2005-12-08

申请号：US11137385

申请日：2005-05-26

申请人： Gary Griffiths

发明人： Gary Griffiths

IPC分类号： A61K39/395 ,  A61K47/48 ,  C07K16/46

CPC分类号： A61K47/6849 ,  A61K47/6809 ,  A61K47/6889

摘要： The invention relates to therapeutic conjugates with the ability to target various antigens. The conjugates contain a targeting antibody or antigen binding fragment thereof and an anthracycline chemotherapeutic drug. The targeting antibody and the chemotherapeutic drug are linked via a linker comprising a hydrazide moiety.

摘要翻译： 本发明涉及具有靶向各种抗原能力的治疗性缀合物。 缀合物含有靶向抗体或其抗原结合片段和蒽环类化疗药物。 靶向抗体和化学治疗药物通过包含酰肼部分的接头连接。

公开(公告)号：US20050191239A1

公开(公告)日：2005-09-01

申请号：US11000500

申请日：2004-12-01

申请人： Gary Griffiths ,  Serengulam Govindan

发明人： Gary Griffiths ,  Serengulam Govindan

IPC分类号： A61K51/00 ,  A61K51/10 ,  C07K14/52 ,  C07K14/525 ,  C07K14/54 ,  C07K16/46

CPC分类号： A61K51/1027 ,  A61K51/0482 ,  A61K51/1045

摘要： Improved synthesis methods are provided for preparing conjugates of proteins and chelating agents. In the synthesis methods, non-stable bonds are hydrolyzed after the conjugate is synthesized, for example by treating the conjugate with a quenching agent. The synthesis method provides conjugates that are substantially free of non-stable bonds between the protein and the chelating agent, such that the chelating agent is less likely to dissociate with the conjugate after the conjugate has been stored for extended periods. The conjugate may be useful for therapeutic or diagnostic methods.

摘要翻译： 提供改进的合成方法用于制备蛋白质和螯合剂的缀合物。 在合成方法中，在合成共轭物后，例如通过用猝灭剂处理缀合物，非稳定键被水解。 合成方法提供基本上不含蛋白质和螯合剂之间的非稳定键的缀合物，使得螯合剂在缀合物长时间储存​​后不太可能与缀合物解离。 缀合物可用于治疗或诊断方法。