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公开(公告)号：US10859563B2
公开(公告)日：2020-12-08
申请号：US14956067
申请日：2015-12-01
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Jessica Godin Karp , Xiaohui Chen , Kashan Ali Shaikh , Mengli Wang , Ralf Lenigk , Christine Lynne Surrette
Abstract: A device and/or methodology are described that include a mechanism for separating erythrocytes from other constituents of blood and for purifying leukocytes from blood. The separation and purification aspects may be provided in separate components or within the same component. The separation aspect assists in separating erythrocytes (red blood cells) from other cells in blood, such as by aggregation of the red blood cells. A suitable aggregation device or device component uses chambers with at least one small dimension (e.g., a microfluidic chip) to control the interaction of the blood with a solution containing a high molecular weight polymer (e.g., dextran) to achieve separation.
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公开(公告)号：US11821891B2
公开(公告)日：2023-11-21
申请号：US17113527
申请日：2020-12-07
Applicant: General Electric Company
Inventor： Jessica Godin Karp , Xiaohui Chen , Kashan Ali Shaikh , Mengli Wang , Ralf Lenigk , Christine Lynne Surrette
CPC classification number: G01N33/5002 , B01L3/502715 , B01L3/502738 , B01L3/502761 , B01L3/502769 , C12Q1/24 , G01N33/491 , B01D2313/18 , B01D2313/50 , B01L2200/0647 , B01L2300/16 , G01N2001/4088
Abstract: A device and/or methodology are described that include a mechanism for separating erythrocytes from other constituents of blood and for purifying leukocytes from blood. The separation and purification aspects may be provided in separate components or within the same component. The separation aspect assists in separating erythrocytes (red blood cells) from other cells in blood, such as by aggregation of the red blood cells. A suitable aggregation device or device component uses chambers with at least one small dimension (e.g., a microfluidic chip) to control the interaction of the blood with a solution containing a high molecular weight polymer (e.g., dextran) to achieve separation.
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公开(公告)号：US20210088503A1
公开(公告)日：2021-03-25
申请号：US17113527
申请日：2020-12-07
Applicant: General Electric Company
Inventor： Jessica Godin Karp , Xiaohui Chen , Kashan Ali Shaikh , Mengli Wang , Ralf Lenigk , Christine Lynne Surrette
Abstract: A device and/or methodology are described that include a mechanism for separating erythrocytes from other constituents of blood and for purifying leukocytes from blood. The separation and purification aspects may be provided in separate components or within the same component. The separation aspect assists in separating erythrocytes (red blood cells) from other cells in blood, such as by aggregation of the red blood cells. A suitable aggregation device or device component uses chambers with at least one small dimension (e.g., a microfluidic chip) to control the interaction of the blood with a solution containing a high molecular weight polymer (e.g., dextran) to achieve separation.
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4.发明申请公开(公告)号：US20160123926A1
公开(公告)日：2016-05-05
申请号：US14865478
申请日：2015-09-25
Applicant: General Electric Company
Inventor： John Richard Nelson , Christopher Michael Puleo , Erin Jean Finehout , Patrick McCoy Spooner , Kashan Ali Shaikh , Xiaohui Chen , Li Zhu
IPC: G01N27/447
CPC classification number: G01N27/44791 , C07H1/08 , C07H21/00 , C12N15/1017 , G01N27/44747
Abstract: A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.
Abstract translation: 从生物材料中分离核酸的方法包括将生物材料应用于包含其中浸渍的一种或多种细胞裂解试剂的底物; 将液体施加到施加在基底上的生物材料; 从施加在基底上的生物材料中提取核酸; 并以基本上完整的形式收集所提取的核酸，其中所收集的核酸具有大于或等于20kb的分子量。
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公开(公告)号：US09347033B2
公开(公告)日：2016-05-24
申请号：US14813406
申请日：2015-07-30
Applicant: General Electric Company
Inventor： Zaeem Ashraf Khan , Aaron Joseph Dulgar-Tulloch , Stefan Rakuff , Philip Alexander Shoemaker , Erik Leeming Kvam , Xiaohui Chen , Jaydeep Roy
CPC classification number: C12M45/09 , B01F5/0057 , B01F5/0206 , B01F5/0231 , B01F5/0256 , B01F5/10 , C12M27/00 , C12M47/04 , C12N5/0653 , C12N2501/70 , C12N2501/734 , C12N2521/00
Abstract: A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer, introducing the adipose tissue into the vessel, introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided.
Abstract translation: 提供了一种处理脂肪组织以收集脂肪来源的再生细胞的方法，其中所述方法包括提供包含流体喷射混合器的容器，将脂肪组织引入容器中，将缓冲溶液引入容器中; 使用流体喷射混合器洗涤脂肪组织; 将酶溶液引入容器中; 使用流体喷射混合器将包含脂肪组织，酶溶液和缓冲溶液的射流混合到消化脂肪组织以形成消化产物; 将消化产物相分离成消化的浮力脂肪层和非浮力水层; 并收集包含脂肪来源的再生细胞的非浮力水层。 还提供了一种处理脂肪组织以收集脂肪来源的再生细胞的系统。
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Patent Agency Ranking
6.发明申请公开(公告)号：US20150094548A1
公开(公告)日：2015-04-02
申请号：US14039497
申请日：2013-09-27
Applicant: General Electric Company
Inventor： James Enrico Sabatini , Jeffrey Michael Ashe , Xiaohui Chen , Tzu-Jen Kao
CPC classification number: A61B5/0053 , A61B5/0537 , A61B5/4875 , A61B5/4878
Abstract: In accordance with one aspect of the present technique, a method is disclosed. The method includes applying a mechanical perturbation to a tissue region using a displacement device. The method further includes calculating a compression impedance of the tissue region in response to applying the mechanical perturbation. The method further includes retracting the displacement device and calculating a retraction impedance of the tissue region in response to retracting the displacement device. The method also includes determining a hydration level of the tissue region based on at least one of the compression impedance and the retraction impedances.
Abstract translation: 根据本技术的一个方面，公开了一种方法。 该方法包括使用位移装置将机械扰动施加到组织区域。 该方法还包括响应于施加机械扰动来计算组织区域的压缩阻抗。 该方法还包括回缩位移装置并响应于缩回位移装置计算组织区域的缩回阻抗。 该方法还包括基于压缩阻抗和回缩阻抗中的至少一个来确定组织区域的水化水平。
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公开(公告)号：US20150002168A1
公开(公告)日：2015-01-01
申请号：US13929362
申请日：2013-06-27
Applicant: General Electric Company
Inventor： Tzu-Jen Kao , David Michael Davenport , Jeffrey Michael Ashe , Xiaohui Chen
IPC: G01R27/02
CPC classification number: A61B5/0536 , A61B5/4875 , A61B5/4878
Abstract: One system includes a pattern generator that generates one or more excitation patterns suitable for probing a hydration level of a tissue of a subject at one or more depths from a surface of the subject into an interrogation region. Each of the excitation patterns has a spatial sensitivity at one of the one or more predetermined depths. A data analysis module receives one or more measured responses of the subject at a plurality of electrodes to excitation applied by the plurality of electrodes based on the one or more excitation patterns and determines one or more hydration changes at the one or more depths within the subject based on the measured responses. Each of the measured responses corresponds to the one of the one or more predetermined depths for which the applied excitation pattern has spatial sensitivity.
Abstract translation: 一个系统包括图案发生器，其产生一个或多个激励图案，所述激励图案适于探测从被摄体表面到询问区域的一个或多个深度处的受试者的组织的水化水平。 每个激发图案在一个或多个预定深度中的一个处具有空间灵敏度。 数据分析模块基于一个或多个激励图案，在多个电极处接收受试者在多个电极处的一个或多个测量的响应，由多个电极施加的激励，并确定受试者内的一个或多个深度处的一个或多个水合变化 基于测量的响应。 每个测量的响应对应于所施加的激励图案具有空间灵敏度的一个或多个预定深度中的一个。
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公开(公告)号：US09999352B2
公开(公告)日：2018-06-19
申请号：US14039497
申请日：2013-09-27
Applicant: General Electric Company
Inventor： James Enrico Sabatini , Jeffrey Michael Ashe , Xiaohui Chen , Tzu-Jen Kao
CPC classification number: A61B5/0053 , A61B5/0537 , A61B5/4875 , A61B5/4878
Abstract: In accordance with one aspect of the present technique, a method is disclosed. The method includes applying a mechanical perturbation to a tissue region using a displacement device. The method further includes calculating a compression impedance of the tissue region in response to applying the mechanical perturbation. The method further includes retracting the displacement device and calculating a retraction impedance of the tissue region in response to retracting the displacement device. The method also includes determining a hydration level of the tissue region based on at least one of the compression impedance and the retraction impedances.
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公开(公告)号：US20170153223A1
公开(公告)日：2017-06-01
申请号：US14956067
申请日：2015-12-01
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Jessica Godin Karp , Xiaohui Chen , Kashan Ali Shaikh , Mengli Wang , Ralf Lenigk , Christine Lynne Surrette
CPC classification number: G01N33/5002 , B01L3/502715 , B01L3/502738 , B01L3/502761 , B01L3/502769 , B01L2200/0647 , B01L2300/16 , C12Q1/24 , G01N33/491
Abstract: A device and/or methodology are described that include a mechanism for separating erythrocytes from other constituents of blood and for purifying leukocytes from blood. The separation and purification aspects may be provided in separate components or within the same component. The separation aspect assists in separating erythrocytes (red blood cells) from other cells in blood, such as by aggregation of the red blood cells. A suitable aggregation device or device component uses chambers with at least one small dimension (e.g., a microfluidic chip) to control the interaction of the blood with a solution containing a high molecular weight polymer (e.g., dextran) to achieve separation.
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10.发明申请公开(公告)号：US20150337263A1
公开(公告)日：2015-11-26
申请号：US14813406
申请日：2015-07-30
Applicant: General Electric Company
Inventor： Zaeem Ashraf Khan , Aaron Joseph Dulgar-Tulloch , Stefan Rakuff , Philip Alexander Shoemaker , Erik Leeming Kvam , Xiaohui Chen , Jaydeep Roy
IPC: C12N5/077
CPC classification number: C12M45/09 , B01F5/0057 , B01F5/0206 , B01F5/0231 , B01F5/0256 , B01F5/10 , C12M27/00 , C12M47/04 , C12N5/0653 , C12N2501/70 , C12N2501/734 , C12N2521/00
Abstract: A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer, introducing the adipose tissue into the vessel, introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided.
Abstract translation: 提供了一种处理脂肪组织以收集脂肪来源的再生细胞的方法，其中所述方法包括提供包含流体喷射混合器的容器，将脂肪组织引入容器中，将缓冲溶液引入容器中; 使用流体喷射混合器洗涤脂肪组织; 将酶溶液引入容器中; 使用流体喷射混合器将包含脂肪组织，酶溶液和缓冲溶液的射流混合到消化脂肪组织以形成消化产物; 将消化产物相分离成消化的浮力脂肪层和非浮力水层; 并收集包含脂肪来源的再生细胞的非浮力水层。 还提供了一种处理脂肪组织以收集脂肪来源的再生细胞的系统。
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