公开(公告)号：US09834793B2

公开(公告)日：2017-12-05

申请号：US14690542

申请日：2015-04-20

申请人： Evolva SA

发明人： Michael Katz ,  Hans Peter Smits ,  Jochen Forster ,  Jens Bredal Nielsen

IPC分类号： C12P7/22 ,  C12N1/00 ,  C07H21/04 ,  C12P23/00 ,  C12N9/10 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12N15/52 ,  C12P7/42 ,  A23C3/08 ,  A61K31/05

CPC分类号： C12P7/22 ,  A23C3/08 ,  A61K31/05 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/1029 ,  C12N9/1037 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/42 ,  C12P23/00 ,  C12Y106/02004 ,  C12Y114/13011 ,  C12Y203/01074 ,  C12Y403/01005 ,  C12Y602/01012 ,  C12Y604/01002 ,  Y02A50/473 ,  Y02P20/52

摘要： A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.

公开(公告)号：US09725743B2

公开(公告)日：2017-08-08

申请号：US13732887

申请日：2013-01-02

申请人： Evolva SA

发明人： Michael Katz ,  Jochen Forster ,  Helga David ,  Hans Peter Schmidt ,  Malin Sendelius ,  Sara Peterson Bjorn ,  Thomas Thomasen Durhuus

IPC分类号： C12P7/22 ,  C12N9/10 ,  C12N9/88 ,  C12N9/00

CPC分类号： C12P7/22 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/93

摘要： A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4 -hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.

公开(公告)号：US09404129B2

公开(公告)日：2016-08-02

申请号：US14514718

申请日：2014-10-15

申请人： Evolva SA

发明人： Michael Katz ,  Hans Peter Smits ,  Jochen Forster ,  Jens Bredel Nielsen

IPC分类号： C12N1/20 ,  C12N1/00 ,  C12P7/22 ,  C07H21/04 ,  C12P23/00 ,  C12N9/10 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12N15/52 ,  C12P7/42 ,  A23C3/08 ,  A61K31/05

CPC分类号： C12P7/22 ,  A23C3/08 ,  A61K31/05 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/1029 ,  C12N9/1037 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/42 ,  C12P23/00 ,  C12Y106/02004 ,  C12Y114/13011 ,  C12Y203/01074 ,  C12Y403/01005 ,  C12Y602/01012 ,  C12Y604/01002 ,  Y02A50/473 ,  Y02P20/52

摘要： A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.