公开(公告)号：US06272203B1

公开(公告)日：2001-08-07

申请号：US09368413

申请日：1999-08-05

Applicant: Eiji Yoneda ,  Yasuhisa Asano

Inventor： Eiji Yoneda ,  Yasuhisa Asano

IPC: G01N23223

CPC classification number: G01N23/223 ,  G01N2223/076

Abstract: The apparatus enables measurement of the amount of contaminant in a welded portion to high accuracy and in a short time, using an X-ray fluorescence inspection apparatus. The end portion of a fuel rod having a bead portion on an end surface of an end plug is covered with an approximately cylindrical cap, with the bead portion exposed via a central aperture. The material of the cap is preferably titanium. With titanium the fluorescent X-ray radiation has a different wavelength from that of tungsten and zircaloy, and the amount of fluorescent X-ray radiation detected by a detector at a diffraction angle of the spectrum W-L &agr; of fluorescent X-ray radiation for tungsten is less than those for tungsten and zircaloy. Therefore, while the fluorescent X-ray radiation of the cap which covers the end surface of the end plug around the bead portion results in noise, the amount of this fluorescent X-ray radiation measured by the detector is much smaller than for the tungsten and the zircaloy of the bead portion.

Abstract translation: 该装置能够使用X射线荧光检查装置，以高精度和短时间测量焊接部分中的污染物的量。 具有在端塞的端面上的胎圈部分的燃料棒的端部被大致圆筒形的盖子覆盖，胎圈部分通过中心孔露出。 盖的材料优选为钛。 利用钛，荧光X射线辐射具有与钨和锆石的不同的波长，并且由钨的荧光X射线辐射的光谱WLα的衍射角由检测器检测的荧光X射线辐射的量为 小于钨和锆石的。 因此，虽然盖子的荧光X射线辐射覆盖端子塞周围的珠粒部分的端面导致噪音，但由该检测器测得的该荧光X射线辐射的量比钨和 胎圈部分的氧化锆。

公开(公告)号：US20170268033A1

公开(公告)日：2017-09-21

申请号：US15608460

申请日：2017-05-30

Applicant: Yasuhisa Asano ,  Masafumi Kameya ,  Hiroyasu Onaka

Inventor： Yasuhisa Asano ,  Masafumi Kameya ,  Hiroyasu Onaka

IPC: C12Q1/26

CPC classification number: C12Q1/26 ,  C12Y104/03

Abstract: Disclosed is a method for quantifying L-tryptophan involving a step for mixing a specimen, L-tryptophan oxidase, and water, a step for allowing the obtained reaction solution to stand a predetermined period of time in the presence of oxygen, and a step for measuring the reaction product resulting from action of enzymes present in the reaction solution after allowing to stand. The L-tryptophan oxidase has a given amino acid sequence and has oxidase activity that generates hydrogen peroxide and ammonia by acting on the L-tryptophan in the presence of oxygen and water. The oxidase activity of the L-tryptophan oxidase on the L-phenylalanine is in the range of 0-3% of the oxidase activity thereof on the L-tryptophan, and the L-tryptophan oxidase does not have oxidase activity on protein-constituting amino acids other than L-tryptophan and L-phenylalanine. Also disclosed are a kit used to quantify the L-tryptophan containing L-tryptophan oxidase, and an enzyme sensor using the L-tryptophan oxidase. This method, kit and enzyme sensor use an L-tryptophan-specific enzyme, so are capable of quantifying L-tryptophan even in the presence of other amino acids.

公开(公告)号：US09181574B2

公开(公告)日：2015-11-10

申请号：US14445597

申请日：2014-07-29

Applicant: Yasuhisa Asano ,  Daisuke Matsui

Inventor： Yasuhisa Asano ,  Daisuke Matsui

IPC: C12N9/06 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/28

CPC classification number: C12Q1/005 ,  C12N9/0022 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2533/00

Abstract: Methods are provided measuring L-lysine using a variant enzyme, an L-lysine measurement kit, and an enzyme sensor. Variant L-amino acid oxidase having a predetermined amino acid mutation, and having oxidase activity that is highly substrate-specific for L-lysine; a method for measuring L-lysine using this variant enzyme; an L-lysine measurement kit; and an enzyme sensor are also provided.

Abstract translation: 提供使用变体酶，L-赖氨酸测量试剂盒和酶传感器测量L-赖氨酸的方法。 具有预定氨基酸突变的具有对L-赖氨酸具有高度底物特异性的氧化酶活性的变体L-氨基酸氧化酶; 使用该变体酶测定L-赖氨酸的方法; L-赖氨酸测量试剂盒; 还提供酶传感器。

公开(公告)号：US07776570B2

公开(公告)日：2010-08-17

申请号：US12174995

申请日：2008-07-17

Applicant: Yasuhisa Asano ,  Atsushi Inoue

Inventor： Yasuhisa Asano ,  Atsushi Inoue

IPC: C12P13/04 ,  C12N9/78 ,  C12N9/80 ,  C07H21/04

CPC classification number: C12P13/04 ,  C12N9/80 ,  C12P13/12 ,  C12P41/006 ,  C12Y305/01004

Abstract: A recombinant microorganism is produced by introducing a DNA encoding an enzyme which hydrolyzes an amido bond of L-amino acid amide, especially L-2-alkylcysteine amide, and L-amino acid is produced by using cells or cell processed product of the obtained microorganism.

Abstract translation: 通过引入编码水解L-氨基酸酰胺的酰胺键的酶，特别是L-2-烷基半胱氨酸酰胺的DNA，通过使用得到的微生物的细胞或细胞加工产物产生L-氨基酸来制备重组微生物 。

公开(公告)号：US06246741B1

公开(公告)日：2001-06-12

申请号：US09258443

申请日：1999-02-26

Applicant: Kohichi Nunokawa ,  Yasuhisa Asano

Inventor： Kohichi Nunokawa ,  Yasuhisa Asano

IPC: G21C330

CPC classification number: G21C7/20 ,  Y02E30/32 ,  Y02E30/39

Abstract: A fuel assembly for a pressurized water reactor having control rod guide thimbles (5) each having a dashpot (12) for protecting against flexural deformation which may impair insertability of a control rod The fuel assembly includes, a plurality of control rod guide thimbles (5) having bottom and top end portions fixedly secured to a lower nozzle (2) and an upper nozzle (4), respectively, disposed in opposition to each other. The dashpot (12) of each control rod guide thimble (5) includes a small diameter section (13b) having an outer diameter smaller than that of the control rod guide thimble (5) formed at an upper portion of the dashpot (12), and a large diameter section (13a) having an outer diameter substantially equal to that of the control rod guide thimble (5) formed at a lower portion of the dashpot (12). With the length of the control rod guide thimble (5) represented by L, the effective length (S) of the small diameter section (13b) is selected to lie within a range of from 0.03 L to 0.1 L.

Abstract translation: 一种用于具有控制杆导向套管（5）的压力水反应器的燃料组件，每个具有缓冲器（12），用于防止可能损害控制棒的可插入性的弯曲变形。燃料组件包括多个控制杆导向套管（5 ），其底部和顶端部分分别固定地固定到彼此相对设置的下喷嘴（2）和上喷嘴（4）。 每个控制杆导向套管（5）的缓冲器（12）包括一个小直径部分（13b），其外径小于形成在缓冲器（12）的上部处的控制杆导向套管（5）的外径， 以及具有与形成在缓冲器（12）的下部的控制棒导向套管（5）的外径大致相等的外径的大直径部（13a）。 以L表示的控制杆导向套管（5）的长度，小直径部分（13b）的有效长度（S）选择在0.03L至0.1L的范围内。

公开(公告)号：US4970157A

公开(公告)日：1990-11-13

申请号：US84238

申请日：1987-08-11

Applicant: Yasuo Hibino ,  Yasuhisa Asano ,  Noriko Okazaki ,  Naganori Numao

Inventor： Yasuo Hibino ,  Yasuhisa Asano ,  Noriko Okazaki ,  Naganori Numao

IPC: C12N1/21 ,  C12N9/06 ,  C12N15/53 ,  C12P13/22

CPC classification number: C12N9/0018 ,  C12N9/0028 ,  C12P13/222

Abstract: An isolated gene coding for phenylalanine dehydrogenase of a microorganism belonging to a genus selected from the group consisting of the genera Bacillus and Sporosarcina origin; plasmids containing the gene; microorganism transformed with the plasmid, a process for the production of phenylalanine dehydrogenase using the microogranism; and a process for the production of L-phenylalanine using the enzyme.

Abstract translation: 编码属于选自芽孢杆菌属和孢子纲属的属的微生物的苯丙氨酸脱氢酶的分离基因; 含有该基因的质粒; 用质粒转化的微生物，使用微观色谱法生产苯丙氨酸脱氢酶的方法; 以及使用该酶生产L-苯丙氨酸的方法。

公开(公告)号：US20140357524A1

公开(公告)日：2014-12-04

申请号：US14453189

申请日：2014-08-06

Applicant: Yasuhisa Asano ,  Masafumi Kameya

Inventor： Yasuhisa Asano ,  Masafumi Kameya

IPC: C12Q1/48 ,  C12Q1/32 ,  C12Q1/34

CPC classification number: C12Q1/485 ,  C12Q1/25 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/48 ,  G01N33/6812 ,  G01N33/6815

Abstract: There is provided a method for quantifying a subject substance, of which typical examples are amino acids. The method of the present invention comprises the following steps: the step of allowing an enzyme that can generate pyrophosphate by using adenosine triphosphate (ATP) as a substrate with converting the subject substance to act on the subject substance to generate pyrophosphate; the step of allowing pyruvate pyrophosphate dikinase (PPDK) to act on the generated pyrophosphate in the presence of adenosine monophosphate (AMP) and phosphoenolpyruvate (PEP) to generate ATP, phosphoric acid, and pyruvate; and the step of quantifying the generated pyruvate, and amount of the subject substance is determined on the basis of the obtained amount of pyruvate. According to the present invention, an amino acid in a biological sample containing a lot of various kinds of contaminants such as inorganic phosphoric acid and urea can be conveniently and quickly quantified without being influenced by the contaminants.

Abstract translation: 本发明提供了一种定量目标物质的方法，其中典型的实例是氨基酸。 本发明的方法包括以下步骤：通过使用三磷酸腺苷（ATP）作为底物，使可以产生焦磷酸盐的酶转化为目标物质以产生焦磷酸盐; 在磷酸腺苷（AMP）和磷酸烯醇丙酮酸（PEP）存在下，使丙酮酸焦磷酸二激酶（PPDK）作用于产生的焦磷酸盐以产生ATP，磷酸和丙酮酸盐的步骤; 并且基于获得的丙酮酸的量来确定定量产生的丙酮酸的步骤和目标物质的量。 根据本发明，可以方便快速地定量含有大量各种污染物如无机磷酸和尿素的生物样品中的氨基酸，而不受污染物的影响。

公开(公告)号：US08409841B2

公开(公告)日：2013-04-02

申请号：US13210630

申请日：2011-08-16

Applicant: Yasuhisa Asano ,  Takanori Akiyama ,  Fujio Yu ,  Eiji Sato

Inventor： Yasuhisa Asano ,  Takanori Akiyama ,  Fujio Yu ,  Eiji Sato

IPC: C12N9/00 ,  C12N9/88 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC classification number: C12N9/88 ,  C12P7/42 ,  C12P13/004

Abstract: An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture.

公开(公告)号：US20130052679A1

公开(公告)日：2013-02-28

申请号：US13602473

申请日：2012-09-04

Applicant: Yasuhisa Asano ,  Techawaree Ueatrongchit

Inventor： Yasuhisa Asano ,  Techawaree Ueatrongchit

IPC: C12Q1/32 ,  C12N15/63 ,  C12N1/21 ,  C12N15/53 ,  C12N1/19 ,  C12N1/15 ,  G01N21/75 ,  G01N21/64 ,  C12N9/04 ,  C12N5/10

CPC classification number: C12Q1/32 ,  C12N9/0006 ,  C12Q1/005

Abstract: A method for analyzing L-threonine contained in an specimen, which includes the steps of mixing a sample containing the specimen with an L-threonine dehydrogenase derived from Cupriavidus necator and a coenzyme NAD+ and analyzing the amount of NADH or 2-amino-3-oxobutyric acid after a predetermined period; an L-threonine dehydrogenase derived from Cupriavidus necator, which is a novel L-threonine dehydrogenase (TDH; EC 1.1.1.103) and can be utilized in the above-mentioned analysis method; a method for preparing a gene or the like to be used in the preparation of the enzyme, or a method for preparing the enzyme; an L-threonine analysis kit which includes (A) the L-threonine dehydrogenase and (B) a coenzyme NAD+; an enzyme preparation for use in the analysis of L-threonine, which includes the L-threonine dehydrogenase contained in a buffer solution; and an enzyme sensor utilizing the L-threonine dehydrogenase.

Abstract translation: 一种用于分析样品中所含的L-苏氨酸的方法，其包括以下步骤：将含有样品的样品与源自Cupriavidus Necator的L-苏氨酸脱氢酶和辅酶NAD +混合，并分析NADH或2-氨基-3- 预定时间后的氧代丁酸; 衍生自Cupriavidus Necator的L-苏氨酸脱氢酶，其是新型L-苏氨酸脱氢酶（TDH; EC 1.1.1.103），可用于上述分析方法; 制备用于制备酶的基因等的方法或其制备方法; 一种L-苏氨酸分析试剂盒，其包括（A）L-苏氨酸脱氢酶和（B）辅酶NAD +; 用于分析L-苏氨酸的酶制剂，其包括缓冲溶液中所含的L-苏氨酸脱氢酶; 和利用L-苏氨酸脱氢酶的酶传感器。

公开(公告)号：US20130005007A1

公开(公告)日：2013-01-03

申请号：US13519058

申请日：2011-02-14

Applicant: Yasuhisa Asano ,  Kenichi Fuhshuku ,  Tetsuro Nishii ,  Akihiro Ishii

Inventor： Yasuhisa Asano ,  Kenichi Fuhshuku ,  Tetsuro Nishii ,  Akihiro Ishii

IPC: C12P7/04

CPC classification number: C12P7/04 ,  C12N1/16 ,  C12N15/81

Abstract: Disclosed is a method for producing (S)-1,1,1-trifluoro-2-propanol with high optical purity and high yield by having at least one kind of microorganism, which is selected from the group consisting of Hansenula polymorpha, Pichia anomala, Candida parapsilosis, Candida mycoderma, Pichia naganishii, Candida saitoana, Cryptococcus curvatus, Saturnospora dispora, Saccharomyces bayanus and Pichia membranaefaciens, act on 1,1,1-trifluoroacetone. Since microorganisms found in nature are made to act in a natural state, the problems to be raised when a transformant or the like is used can be avoided in this method. Consequently, the method can be easily put in industrial practice.

Abstract translation: 公开了具有高光学纯度和高产率的（S）-1,1,1-三氟-2-丙醇的方法，该方法具有至少一种微生物，其选自多形汉逊酵母（Hansenula polymorpha），异形毕赤酵母 ，奇异假丝酵母（Candida parapsilosis），真菌念珠菌（Candida mycoderma），日本毕赤酵母（Pichia naganishii），蚕豆假丝酵母（Candida saitoana），曲霉隐球菌（Cryptococcus curvatus），土星孢子（Saturnospora dispora），巴豆酵母（Saccharomyces bayanus）和毕赤酵母（Pichia membranaefaciens），作用于1,1,1-三氟丙酮。 由于在自然界中发现的微生物使自然状态发生作用，所以在这种方法中可以避免使用转化体等引起的问题。 因此，该方法可以轻松投入工业实践。