公开(公告)号：US20100221788A1

公开(公告)日：2010-09-02

申请号：US12548830

申请日：2009-08-27

申请人： Edmund RADMACHER ,  Klaus Moller

发明人： Edmund RADMACHER ,  Klaus Moller

IPC分类号： C12P19/34 ,  C07H21/04 ,  C12N9/16

CPC分类号： C12N15/1006

摘要： The invention relates to a method for recovering at least short RNA, having at least the following steps: a) making available a biological solution containing at least short RNA as well as proteins and/or long nucleic acids (long RNA and DNA); b) removing the proteins and the long nucleic acids from the solution, at least the proteins being precipitated; c) adsorbing the short RNA onto a solid (first) carrier after precipitation of the proteins; d) recovering the short RNA by desorption from the carrier. The invention further includes a kit for carrying out the method.

摘要翻译： 本发明涉及至少回收短RNA的方法，其至少具有以下步骤：a）提供至少含有短RNA以及蛋白质和/或长核酸（长RNA和DNA）的生物溶液; b）从溶液中去除蛋白质和长核酸，至少蛋白质被沉淀; c）在蛋白沉淀后将短RNA吸附到固体（第一）载体上; d）通过从载体解吸而回收短RNA。 本发明还包括用于执行该方法的套件。