公开(公告)号：US20240110240A1

公开(公告)日：2024-04-04

申请号：US18481950

申请日：2023-10-05

申请人： ENUMERIX, INC.

发明人： Eleen Yee Lam SHUM ,  Hei Mun Christina FAN ,  Stephen P.A. FODOR ,  Janice Hoiyi LAI ,  Jung Won KEUM ,  Haeun Grace LEE

IPC分类号： C12Q1/6876 ,  G01N21/64 ,  G01N33/542

CPC分类号： C12Q1/6876 ,  G01N21/6428 ,  G01N33/542 ,  C12Q2600/156 ,  C12Q2600/16 ,  G01N2021/6439

摘要： The disclosure provides compositions, methods, and systems for implementation of highly multiplexed molecular diagnostic assays involving color combinatorics, stimulus-responsive probes, tandem probes, conjugated polymer probes, and other mechanisms for increasing the number of targets that can be simultaneously detected in a digital assay. Multiplexed detection of targets is achieved in a rapid manner, with respect to sample partitioning and target detection using multiple color channels for detection. Implementation of methods described also achieve detection with significantly improved signal-to-noise ratio (SNR) values.

公开(公告)号：US20230287482A1

公开(公告)日：2023-09-14

申请号：US17985485

申请日：2022-11-11

申请人： Enumerix, Inc.

发明人： Hei Mun Christina FAN ,  Eleen Yee Lam SHUM ,  Janice Hoiyi LAI ,  Stephen P. A. FODOR

IPC分类号： C12Q1/6851 ,  C12Q1/686 ,  G01N1/38 ,  B01L3/00

CPC分类号： C12Q1/6851 ,  B01L3/5023 ,  C12Q1/686 ,  G01N1/38

摘要： The inventions cover systems and methods for generation of emulsions having suitable clarity without requiring refractive index matching between emulsion components. Systems can include: a substrate including a set of openings; a reservoir facing the substrate at a first side and containing a sample fluid configured for droplet formation upon interacting with the set of openings of the substrate; and a collecting container facing the substrate at a second side and containing a set of fluid layers configured with a density gradient and suitable immiscibility characteristics. One or more components of the system(s) can support methods for emulsion generation, in relation to enabling interactions between multiple continuous phases and a dispersed droplet phase to generate clear emulsions. Applications of the inventions(s) can include performance of droplet-based digital PCR in an improved manner (e.g., without requiring implementation of correction factors based upon Poisson statistics).

公开(公告)号：US20240352523A1

公开(公告)日：2024-10-24

申请号：US18759368

申请日：2024-06-28

申请人： ENUMERIX, INC.

发明人： Eleen Yee Lam SHUM ,  Hei Mun Christina FAN ,  Stephen P.A. FODOR ,  Janice Hoiyi LAI ,  Jung Won KEUM ,  Haeun Grace LEE

IPC分类号： C12Q1/6876 ,  G01N21/64 ,  G01N33/542

CPC分类号： C12Q1/6876 ,  G01N21/6428 ,  G01N33/542 ,  C12Q2600/156 ,  C12Q2600/16 ,  G01N2021/6439

摘要： This disclosure provides for devices, methods, and systems for performing a non-invasive prenatal testing (NIPT) digital assay upon generating at least a large number of counts per chromosome for a set of chromosomes present in a sample, where performing the NIPT digital assay can include: distributing nucleic acids of the sample and materials for an amplification reaction across a plurality of partitions; amplifying the nucleic acids with the materials, within the plurality of partitions; and generating counts per chromosome upon detecting signals from the plurality of partitions. The inventions enable processing of samples for NIPT digital analyses and/or other digital analyses involving other loci of interest, with unprecedented partitioning, reaction, readout, and analytical performance.

公开(公告)号：US20220186302A1

公开(公告)日：2022-06-16

申请号：US17561608

申请日：2021-12-23

申请人： Enumerix, Inc.

发明人： Hei Mun Christina FAN ,  Eleen Yee Lam SHUM ,  Janice Hoiyi LAI ,  Stephen P. A. FODOR

IPC分类号： C12Q1/6851 ,  C12Q1/686 ,  G01N1/38 ,  B01L3/00

摘要： The inventions cover systems and methods for generation of emulsions having suitable clarity without requiring refractive index matching between emulsion components. Systems can include: a substrate including a set of openings; a reservoir facing the substrate at a first side and containing a sample fluid configured for droplet formation upon interacting with the set of openings of the substrate; and a collecting container facing the substrate at a second side and containing a set of fluid layers configured with a density gradient and suitable immiscibility characteristics. One or more components of the system(s) can support methods for emulsion generation, in relation to enabling interactions between multiple continuous phases and a dispersed droplet phase to generate clear emulsions. Applications of the inventions(s) can include performance of droplet-based digital PCR in an improved manner (e.g., without requiring implementation of correction factors based upon Poisson statistics).

公开(公告)号：US20220170085A1

公开(公告)日：2022-06-02

申请号：US17673598

申请日：2022-02-16

申请人： Enumerix, Inc.

发明人： Hei Mun Christina FAN ,  Eleen Yee Lam SHUM ,  Janice Hoiyi LAI ,  Stephen P. A. FODOR

IPC分类号： C12Q1/6851 ,  C12Q1/686 ,  G01N1/38 ,  B01L3/00

摘要： The inventions cover systems and methods for generation of emulsions having suitable clarity without requiring refractive index matching between emulsion components. Systems can include: a substrate including a set of openings; a reservoir facing the substrate at a first side and containing a sample fluid configured for droplet formation upon interacting with the set of openings of the substrate; and a collecting container facing the substrate at a second side and containing a set of fluid layers configured with a density gradient and suitable immiscibility characteristics. One or more components of the system(s) can support methods for emulsion generation, in relation to enabling interactions between multiple continuous phases and a dispersed droplet phase to generate clear emulsions. Applications of the inventions(s) can include performance of droplet-based digital PCR in an improved manner (e.g., without requiring implementation of correction factors based upon Poisson statistics).

公开(公告)号：US20230212561A1

公开(公告)日：2023-07-06

申请号：US18093721

申请日：2023-01-05

申请人： Enumerix, Inc.

发明人： Eleen Yee Lam SHUM ,  Hei Mun Christina FAN ,  Stephen P.A. FODOR

IPC分类号： C12N15/10 ,  C12Q1/6806

CPC分类号： C12N15/1093 ,  C12Q1/6806

摘要： The disclosure provides compositions, methods, and systems for extremely accurate generation of nucleic acid libraries, without use of bulk amplification methods. Accurate library preparation is achieved in a rapid manner, with respect to sample partitioning and amplification in a manner that achieves high performance in relation to low levels of amplification bias and low levels of artifact/chimeric sequence generation. Implementation of methods described also achieve library preparation with significantly reduced false positive rates, across a wide variety of applications.

公开(公告)号：US20230193385A1

公开(公告)日：2023-06-22

申请号：US18085217

申请日：2022-12-20

申请人： Enumerix, Inc.

发明人： Eleen Yee Lam SHUM ,  Hei Mun Christina FAN ,  Stephen P.A. FODOR ,  Janice Hoiyi LAI ,  Jung Won KEUM ,  Haeun Grace LEE

IPC分类号： C12Q1/6876 ,  G01N21/64 ,  G01N33/542

CPC分类号： C12Q1/6876 ,  G01N21/6428 ,  G01N33/542 ,  G01N2021/6439 ,  C12Q2600/16 ,  C12Q2600/156

摘要： The disclosure provides compositions, methods, and systems for implementation of highly multiplexed molecular diagnostic assays involving color combinatorics, stimulus-responsive probes, tandem probes, conjugated polymer probes, and other mechanisms for increasing the number of targets that can be simultaneously detected in a digital assay. Multiplexed detection of targets is achieved in a rapid manner, with respect to sample partitioning and target detection using multiple color channels for detection. Implementation of methods described also achieve detection with significantly improved signal-to-noise ratio (SNR) values.

公开(公告)号：US20220411857A1

公开(公告)日：2022-12-29

申请号：US17836736

申请日：2022-06-09

申请人： Enumerix, Inc.

发明人： Hei Mun Christina FAN ,  Eleen Yee Lam SHUM

IPC分类号： C12Q1/6848

摘要： Described herein are methods for amplifying and detecting a specific nucleic acid in a nucleic acid sample. The methods may comprise amplifying a target nucleic acid and detecting the target nucleic acid in the presence of a fluorescent probe and a probe complement. Also described herein are probe complements for detecting specific nucleic acid sequences with reduced background fluorescence.