公开(公告)号：US20240352523A1

公开(公告)日：2024-10-24

申请号：US18759368

申请日：2024-06-28

申请人： ENUMERIX, INC.

发明人： Eleen Yee Lam SHUM ,  Hei Mun Christina FAN ,  Stephen P.A. FODOR ,  Janice Hoiyi LAI ,  Jung Won KEUM ,  Haeun Grace LEE

IPC分类号： C12Q1/6876 ,  G01N21/64 ,  G01N33/542

CPC分类号： C12Q1/6876 ,  G01N21/6428 ,  G01N33/542 ,  C12Q2600/156 ,  C12Q2600/16 ,  G01N2021/6439

摘要： This disclosure provides for devices, methods, and systems for performing a non-invasive prenatal testing (NIPT) digital assay upon generating at least a large number of counts per chromosome for a set of chromosomes present in a sample, where performing the NIPT digital assay can include: distributing nucleic acids of the sample and materials for an amplification reaction across a plurality of partitions; amplifying the nucleic acids with the materials, within the plurality of partitions; and generating counts per chromosome upon detecting signals from the plurality of partitions. The inventions enable processing of samples for NIPT digital analyses and/or other digital analyses involving other loci of interest, with unprecedented partitioning, reaction, readout, and analytical performance.

公开(公告)号：US20230193385A1

公开(公告)日：2023-06-22

申请号：US18085217

申请日：2022-12-20

申请人： Enumerix, Inc.

发明人： Eleen Yee Lam SHUM ,  Hei Mun Christina FAN ,  Stephen P.A. FODOR ,  Janice Hoiyi LAI ,  Jung Won KEUM ,  Haeun Grace LEE

IPC分类号： C12Q1/6876 ,  G01N21/64 ,  G01N33/542

CPC分类号： C12Q1/6876 ,  G01N21/6428 ,  G01N33/542 ,  G01N2021/6439 ,  C12Q2600/16 ,  C12Q2600/156

摘要： The disclosure provides compositions, methods, and systems for implementation of highly multiplexed molecular diagnostic assays involving color combinatorics, stimulus-responsive probes, tandem probes, conjugated polymer probes, and other mechanisms for increasing the number of targets that can be simultaneously detected in a digital assay. Multiplexed detection of targets is achieved in a rapid manner, with respect to sample partitioning and target detection using multiple color channels for detection. Implementation of methods described also achieve detection with significantly improved signal-to-noise ratio (SNR) values.

公开(公告)号：US20240110240A1

公开(公告)日：2024-04-04

申请号：US18481950

申请日：2023-10-05

申请人： ENUMERIX, INC.

发明人： Eleen Yee Lam SHUM ,  Hei Mun Christina FAN ,  Stephen P.A. FODOR ,  Janice Hoiyi LAI ,  Jung Won KEUM ,  Haeun Grace LEE

IPC分类号： C12Q1/6876 ,  G01N21/64 ,  G01N33/542

CPC分类号： C12Q1/6876 ,  G01N21/6428 ,  G01N33/542 ,  C12Q2600/156 ,  C12Q2600/16 ,  G01N2021/6439

摘要： The disclosure provides compositions, methods, and systems for implementation of highly multiplexed molecular diagnostic assays involving color combinatorics, stimulus-responsive probes, tandem probes, conjugated polymer probes, and other mechanisms for increasing the number of targets that can be simultaneously detected in a digital assay. Multiplexed detection of targets is achieved in a rapid manner, with respect to sample partitioning and target detection using multiple color channels for detection. Implementation of methods described also achieve detection with significantly improved signal-to-noise ratio (SNR) values.

公开(公告)号：US20230074085A1

公开(公告)日：2023-03-09

申请号：US17939774

申请日：2022-09-07

申请人： Enumerix, Inc.

发明人： Eleen Yee Lam Shum ,  Hei Mun Christina Fan ,  Stephen P. A. Fodor ,  Haeun Grace LEE

IPC分类号： C12Q1/6844 ,  C12Q1/6883

摘要： This disclosure provides for devices, methods, and systems for performing a non-invasive prenatal testing (NIPT) digital assay upon generating at least a large number of counts per chromosome for a set of chromosomes present in a sample, where performing the NIPT digital assay can include: distributing nucleic acids of the sample and materials for an amplification reaction across a plurality of partitions; amplifying the nucleic acids with the materials, within the plurality of partitions; and generating counts per chromosome upon detecting signals from the plurality of partitions. The inventions enable processing of samples for NIPT digital analyses and/or other digital analyses involving other loci of interest, with unprecedented partitioning, reaction, readout, and analytical performance.