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1.
公开(公告)号:US20220186302A1
公开(公告)日:2022-06-16
申请号:US17561608
申请日:2021-12-23
申请人: Enumerix, Inc.
IPC分类号: C12Q1/6851 , C12Q1/686 , G01N1/38 , B01L3/00
摘要: The inventions cover systems and methods for generation of emulsions having suitable clarity without requiring refractive index matching between emulsion components. Systems can include: a substrate including a set of openings; a reservoir facing the substrate at a first side and containing a sample fluid configured for droplet formation upon interacting with the set of openings of the substrate; and a collecting container facing the substrate at a second side and containing a set of fluid layers configured with a density gradient and suitable immiscibility characteristics. One or more components of the system(s) can support methods for emulsion generation, in relation to enabling interactions between multiple continuous phases and a dispersed droplet phase to generate clear emulsions. Applications of the inventions(s) can include performance of droplet-based digital PCR in an improved manner (e.g., without requiring implementation of correction factors based upon Poisson statistics).
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2.
公开(公告)号:US20220170085A1
公开(公告)日:2022-06-02
申请号:US17673598
申请日:2022-02-16
申请人: Enumerix, Inc.
IPC分类号: C12Q1/6851 , C12Q1/686 , G01N1/38 , B01L3/00
摘要: The inventions cover systems and methods for generation of emulsions having suitable clarity without requiring refractive index matching between emulsion components. Systems can include: a substrate including a set of openings; a reservoir facing the substrate at a first side and containing a sample fluid configured for droplet formation upon interacting with the set of openings of the substrate; and a collecting container facing the substrate at a second side and containing a set of fluid layers configured with a density gradient and suitable immiscibility characteristics. One or more components of the system(s) can support methods for emulsion generation, in relation to enabling interactions between multiple continuous phases and a dispersed droplet phase to generate clear emulsions. Applications of the inventions(s) can include performance of droplet-based digital PCR in an improved manner (e.g., without requiring implementation of correction factors based upon Poisson statistics).
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3.
公开(公告)号:US20230287482A1
公开(公告)日:2023-09-14
申请号:US17985485
申请日:2022-11-11
申请人: Enumerix, Inc.
IPC分类号: C12Q1/6851 , C12Q1/686 , G01N1/38 , B01L3/00
CPC分类号: C12Q1/6851 , B01L3/5023 , C12Q1/686 , G01N1/38
摘要: The inventions cover systems and methods for generation of emulsions having suitable clarity without requiring refractive index matching between emulsion components. Systems can include: a substrate including a set of openings; a reservoir facing the substrate at a first side and containing a sample fluid configured for droplet formation upon interacting with the set of openings of the substrate; and a collecting container facing the substrate at a second side and containing a set of fluid layers configured with a density gradient and suitable immiscibility characteristics. One or more components of the system(s) can support methods for emulsion generation, in relation to enabling interactions between multiple continuous phases and a dispersed droplet phase to generate clear emulsions. Applications of the inventions(s) can include performance of droplet-based digital PCR in an improved manner (e.g., without requiring implementation of correction factors based upon Poisson statistics).
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