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公开(公告)号：US12110532B2
公开(公告)日：2024-10-08
申请号：US18170045
申请日：2023-02-16
Applicant: Design-Zyme LLC
Inventor： Peter Albert Petillo , Dwight O'Dell Deay, III
CPC classification number: C12Q1/005 , C07K1/006 , C07K1/1077 , C12N9/0004 , C12N9/0006 , C12P21/005
Abstract: The present invention is broadly concerned with new in vitro glycosylation methods that provide rational approaches for producing glycosylated proteins, and the use of glycosylated proteins. In more detail, the present invention comprises methods of glycosylating a starting protein having an amino sidechain with a nucleophilic moiety, comprising the step of reacting the protein with a carbohydrate having an oxazoline moiety on the reducing end thereof, to covalently bond the amino sidechain of the starting protein with the oxazoline moiety, wherein the glycosylated protein substantially retains the structure and function of the starting protein. Target proteins include oxidase, oxidoreductase and dehydrogenase enzymes. The glycosylated proteins advantageously have molecular weights of at least about 7500 Daltons. In a further embodiment, the present invention concerns the use of glycosylated proteins, fabricated by the methods disclosed herein, in the assembly of amperometric biosensors.
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公开(公告)号：US11643676B2
公开(公告)日：2023-05-09
申请号：US17301590
申请日：2021-04-08
Applicant: Design-Zyme LLC
CPC classification number: C12Q1/005 , C07K1/006 , C07K1/1077 , C12N9/0004 , C12N9/0006 , C12P21/005
Abstract: The present invention is broadly concerned with new in vitro glycosylation methods that provide rational approaches for producing glycosylated proteins, and the use of glycosylated proteins. In more detail, the present invention comprises methods of glycosylating a starting protein having an amino sidechain with a nucleophilic moiety, comprising the step of reacting the protein with a carbohydrate having an oxazoline moiety on the reducing end thereof, to covalently bond the amino sidechain of the starting protein with the oxazoline moiety, wherein the glycosylated protein substantially retains the structure and function of the starting protein. Target proteins include oxidase, oxidoreductase and dehydrogenase enzymes. The glycosylated proteins advantageously have molecular weights of at least about 7500 Daltons. In a further embodiment, the present invention concerns the use of glycosylated proteins, fabricated by the methods disclosed herein, in the assembly of amperometric biosensors.
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公开(公告)号：US20240139312A1
公开(公告)日：2024-05-02
申请号：US18196214
申请日：2023-05-11
Applicant: Design-Zyme LLC
Inventor： Peter Albert Petillo , Dwight O'Dell Deay, III , Michael Gregory Branden , Erik Naylor , Mary Beth Carter
IPC: A61K39/39 , A61K39/215 , A61P31/14 , A61P37/04
CPC classification number: A61K39/39 , A61K39/215 , A61P31/14 , A61P37/04 , A61K2039/55583
Abstract: Vaccine compositions comprising at least one modified immunogen via in vitro glycosylation methods that provide a rational approach for generating glycosylated versions of immunogens via the reducing end of a linear carbohydrate, the reducing end containing an N-acyl-2-amino moiety. Vaccine compositions comprising a plurality of heterologous immunogens associated with a multivalent carrier, wherein at least one immnunogen is glycosylated. Vaccine compositions comprising multivalent carriers and related methods using the vaccine compositions in various therapeutic and prophylactic applications for inducing an immune response against, treating, or preventing a bacterial, viral, fungal, or protozoan infection. Pathogens for which this approach may be useful include, but are not limited to, influenza viruses, rhinoviruses, human immunodeficiency viruses (HIV), respiratory syncytial virus (RSV), coronaviruses, Babesia, Borrelia, Neisseria, and Chlamydia, and the related diseases thereof
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Patent Agency Ranking
公开(公告)号：US20210230661A1
公开(公告)日：2021-07-29
申请号：US17301590
申请日：2021-04-08
Applicant: Design-Zyme LLC
Abstract: The present invention is broadly concerned with new in vitro glycosylation methods that provide rational approaches for producing glycosylated proteins, and the use of glycosylated proteins. In more detail, the present invention comprises methods of glycosylating a starting protein having an amino sidechain with a nucleophilic moiety, comprising the step of reacting the protein with a carbohydrate having an oxazoline moiety on the reducing end thereof, to covalently bond the amino sidechain of the starting protein with the oxazoline moiety, wherein the glycosylated protein substantially retains the structure and function of the starting protein. Target proteins include oxidase, oxidoreductase and dehydrogenase enzymes. The glycosylated proteins advantageously have molecular weights of at least about 7500 Daltons. In a further embodiment, the present invention concerns the use of glycosylated proteins, fabricated by the methods disclosed herein, in the assembly of amperometric biosensors.
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公开(公告)号：US20240156958A1
公开(公告)日：2024-05-16
申请号：US18499765
申请日：2023-11-01
Applicant: Design-Zyme LLC
Inventor： Peter Albert Petillo , Dwight O'Dell Deay, III , Mary Beth Carter
IPC: A61K39/39 , A61K39/018 , A61K39/02 , A61K39/145 , A61K39/21
CPC classification number: A61K39/39 , A61K39/018 , A61K39/0208 , A61K39/145 , A61K39/21 , A61K2039/55555
Abstract: The present invention is broadly concerned with a vaccine composition comprising a central carrier, at least one linear carbohydrate molecule, and at least one immunogen molecule, wherein each of the at least one linear carbohydrate molecule and at least one immunogen molecule are covalently bound to the carrier via respective covalent linkages. Vaccine compositions comprising multivalent carriers and related methods may find various therapeutic and prophylactic applications for inducing an immune response against, treating, or preventing a bacterial, viral, fungal, or protozoan infection, including, but are not limited to, coronaviruses, Lyme Disease, Chlamydia, and the related diseases thereof.
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公开(公告)号：US20230203557A1
公开(公告)日：2023-06-29
申请号：US18170045
申请日：2023-02-16
Applicant: Design-Zyme LLC
Inventor： Peter Albert Petillo , Dwight O'Dell Deay, III
CPC classification number: C12Q1/005 , C12P21/005 , C07K1/1077 , C12N9/0006 , C12N9/0004 , C07K1/006
Abstract: The present invention is broadly concerned with new in vitro glycosylation methods that provide rational approaches for producing glycosylated proteins, and the use of glycosylated proteins. In more detail, the present invention comprises methods of glycosylating a starting protein having an amino sidechain with a nucleophilic moiety, comprising the step of reacting the protein with a carbohydrate having an oxazoline moiety on the reducing end thereof, to covalently bond the amino sidechain of the starting protein with the oxazoline moiety, wherein the glycosylated protein substantially retains the structure and function of the starting protein. Target proteins include oxidase, oxidoreductase and dehydrogenase enzymes. The glycosylated proteins advantageously have molecular weights of at least about 7500 Daltons. In a further embodiment, the present invention concerns the use of glycosylated proteins, fabricated by the methods disclosed herein, in the assembly of amperometric biosensors.
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公开(公告)号：US11021730B2
公开(公告)日：2021-06-01
申请号：US16224795
申请日：2018-12-19
Applicant: Design-Zyme LLC
Abstract: The present invention is broadly concerned with new in vitro glycosylation methods that provide rational approaches for producing glycosylated proteins, and the use of glycosylated proteins. In more detail, the present invention comprises methods of glycosylating a starting protein having an amino sidechain with a nucleophilic moiety, comprising the step of reacting the protein with a carbohydrate having an oxazoline moiety on the reducing end thereof, to covalently bond the amino sidechain of the starting protein with the oxazoline moiety, wherein the glycosylated protein substantially retains the structure and function of the starting protein. Target proteins include oxidase, oxidoreductase and dehydrogenase enzymes. The glycosylated proteins advantageously have molecular weights of at least about 7500 Daltons. In a further embodiment, the present invention concerns the use of glycosylated proteins, fabricated by the methods disclosed herein, in the assembly of amperometric biosensors.
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公开(公告)号：US20190185900A1
公开(公告)日：2019-06-20
申请号：US16224795
申请日：2018-12-19
Applicant: Design-Zyme LLC
CPC classification number: C12Q1/005 , C12P21/005
Abstract: The present invention is broadly concerned with new in vitro glycosylation methods that provide rational approaches for producing glycosylated proteins, and the use of glycosylated proteins. In more detail, the present invention comprises methods of glycosylating a starting protein having an amino sidechain with a nucleophilic moiety, comprising the step of reacting the protein with a carbohydrate having an oxazoline moiety on the reducing end thereof, to covalently bond the amino sidechain of the starting protein with the oxazoline moiety, wherein the glycosylated protein substantially retains the structure and function of the starting protein. Target proteins include oxidase, oxidoreductase and dehydrogenase enzymes. The glycosylated proteins advantageously have molecular weights of at least about 7500 Daltons. In a further embodiment, the present invention concerns the use of glycosylated proteins, fabricated by the methods disclosed herein, in the assembly of amperometric biosensors.
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