公开(公告)号：US4362197A

公开(公告)日：1982-12-07

申请号：US228131

申请日：1981-01-26

申请人： Charles H. Wick ,  Stephen J. Suor

发明人： Charles H. Wick ,  Stephen J. Suor

IPC分类号： B27L5/00 ,  B27M1/00

CPC分类号： B27M1/00 ,  B27L5/00

摘要： A novel process for slicing veneer comprises the steps of (1) submerging a piece of lumber in a water bath maintained at an elevated temperature for a period of time sufficient to eliminate excessive curling upon slicing, (2) slicing a single slice of veneer from the lumber, and (3) returning the lumber to the bath for an additional soak period before running the wood through the veneer slicer. Soaking between each slice produces greater uniformity in the slices, which are dried more easily with consumption of less energy.

摘要翻译： 用于切割单板的新颖方法包括以下步骤：（1）将一块木材浸入保持在高温下的水浴中足以消除切片时的过度卷曲的时间，（2）将单片薄片从 木材，以及（3）将木材返回到浴中以进行额外的浸泡期，然后通过胶合板切片机运行木材。 在每个切片之间浸泡在切片中产生更大的均匀性，其在更少的能量消耗下更容易干燥。

公开(公告)号：US08524155B1

公开(公告)日：2013-09-03

申请号：US13673441

申请日：2012-11-09

申请人： Charles H. Wick

发明人： Charles H. Wick

IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC分类号： G01N1/2202 ,  G01N15/065 ,  G01N2001/2223 ,  G01N2015/0046 ,  Y10T436/11

摘要： An improved system for collecting, detecting, and classifying submicron-sized particles in a sample comprising an Integrated Virus Detection System (IVDS) of the type wherein the detecting means includes a differential mobility analyzer (DMA) and condensation particle counter (CPC), the improvement comprising positioning an aerosol collector or an electrostatic collector between the differential mobility analyzer and the condensation particle counter wherein submicron-sized particles from the environmental sample are collected for further analysis.

摘要翻译： 一种用于在样品中收集，检测和分类亚微米尺寸颗粒的改进系统，其包括其中检测装置包括差示迁移率分析仪（DMA）和冷凝颗粒计数器（CPC）的类型的综合病毒检测系统（IVDS）， 改进包括将微粒聚集体或静电收集器定位在差示迁移率分析仪和缩合颗粒计数器之间，其中收集来自环境样品的亚微米尺寸的颗粒用于进一步分析。

公开(公告)号：US08146446B1

公开(公告)日：2012-04-03

申请号：US12401013

申请日：2009-03-10

申请人： Charles H. Wick

发明人： Charles H. Wick

IPC分类号： G01N1/18

CPC分类号： G01N1/4077 ,  G01N2001/4088

摘要： A concentrator device and method of concentrating a liquid sample may be provided. The concentrator device may include a pressure vessel and a filter element disposed within the pressure vessel. The pressure vessel may include an inlet configured to introduce pressurized air into a first portion of the pressure vessel and a first outlet fluidly coupled with a second portion of the pressure vessel. The first outlet may be adapted to be selectively opened and closed. A second outlet may be configured to receive a capillary tube inserted into the first portion of the pressure vessel. The filter element may be configured to receive a liquid sample to be concentrated. The filter element may substantially separate the first portion of the pressure vessel from the second portion of the pressure vessel and may define a retentate side adjacent to the first portion and a filtrate side adjacent to the second portion. When pressurized air is introduced through the inlet and the first outlet is open, a filtrate of the liquid sample received in the filter element may pass from the retentate side to the filtrate side such that a concentrated retentate of the liquid sample remains on the retentate side. When the first outlet is closed, the concentrated retentate of the liquid sample may be forced out through the capillary tube.

摘要翻译： 可以提供浓缩装置和浓缩液体样品的方法。 集中器装置可以包括设置在压力容器内的压力容器和过滤元件。 压力容器可以包括被配置成将加压空气引入压力容器的第一部分的入口和与压力容器的第二部分流体耦合的第一出口。 第一出口可以适于选择性地打开和关闭。 第二出口可以被配置为接收插入到压力容器的第一部分中的毛细管。 过滤元件可以被配置为接收待浓缩的液体样品。 过滤元件可以将压力容器的第一部分与压力容器的第二部分基本上分开，并且可以限定邻近第一部分的滞留侧和邻近第二部分的滤液侧。 当加压空气通过入口引入并且第一出口打开时，容纳在过滤器元件中的液体样品的滤液可以从渗余物侧通过滤液侧，使得液体样品的浓缩滞留物保留在滞留侧 。 当第一出口关闭时，液体样品的浓缩滞留物可能被迫通过毛细管排出。

公开(公告)号：US08524482B1

公开(公告)日：2013-09-03

申请号：US12435477

申请日：2009-05-05

申请人： Charles H. Wick

发明人： Charles H. Wick

IPC分类号： C12N7/02 ,  C12Q1/70 ,  A61K39/00

CPC分类号： G01N1/34 ,  A61K38/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/00051 ,  C12N2720/00051 ,  C12N2730/00051 ,  C12N2740/00051 ,  C12N2750/00051 ,  C12N2760/00051 ,  C12N2770/00051

摘要： A system for sampling and separating submicron-sized particles to detect the presence of an agent such as viruses in an environmental sample, which includes collecting means for collecting a sample suspected of containing submicron-sized particles from the environment, size separation means receiving the submicron-sized particles from the collecting means for separating the submicron-sized particles based on size into at least one size range, and a microscale particle counter adapted for counting the size separated submicron-sized particles received from the size separation means. The particle counter includes at least one cantilever each corresponding to submicron-sized particles of a particular size range, wherein the cantilever is deflectable from a first to a second position to permit passage of the submicron-sized particle therethrough, and wherein the corresponding deflection of the cantilever generates a count signal.

摘要翻译： 一种用于采样和分离亚微米尺寸颗粒以检测环境样品中诸如病毒的试剂的存在的系统，其包括用于从环境中收集怀疑含有亚微米尺寸颗粒的样品的收集装置，尺寸分离装置接收亚微米 来自收集装置的尺寸的颗粒，用于基于尺寸将亚微米尺寸的颗粒分离成至少一个尺寸范围;以及微尺寸颗粒计数器，其适于对从尺寸分离装置接收的分离的亚微米尺寸的颗粒进行计数。 颗粒计数器包括至少一个悬臂，每个悬臂对应于特定尺寸范围的亚微米尺寸的颗粒，其中悬臂可从第一位置偏转到第二位置以允许亚微米尺寸的颗粒通过其中，并且其中相应的偏转 悬臂产生计数信号。

公开(公告)号：US08412464B1

公开(公告)日：2013-04-02

申请号：US13546282

申请日：2012-07-11

申请人： Charles H. Wick ,  Michael F. Stanford ,  Alan W. Zulich ,  Samir V. Deshpande ,  Rabih E. Jabbour ,  Jacek P. Dworzanski ,  Patrick E. McCubbin

发明人： Charles H. Wick ,  Michael F. Stanford ,  Alan W. Zulich ,  Samir V. Deshpande ,  Rabih E. Jabbour ,  Jacek P. Dworzanski ,  Patrick E. McCubbin

IPC分类号： G01N33/48 ,  G01N33/50

CPC分类号： G01N33/6848 ,  C12Q1/04 ,  G01N33/5005

摘要： A process is provided for identifying a cell type in a sample that includes identification of one or more peptide sequences in the sample. Each peptide sequence is assigned to a protein of known sequence. A matrix of assignments is generated for the presence or absence of each peptide in one or more cells. The matrix of assignments is rearranged according to cell classification. A cell type based on the most probable cell classification is identified.

摘要翻译： 提供了用于鉴定样品中包含样品中一个或多个肽序列的鉴定的细胞类型的方法。 将每个肽序列分配给已知序列的蛋白质。 生成一个或多个细胞中各肽存在或不存在的分配矩阵。 分配矩阵根据细胞分类重新排列。 确定基于最可能的细胞分类的细胞类型。

公开(公告)号：US08309029B1

公开(公告)日：2012-11-13

申请号：US12411679

申请日：2009-03-26

申请人： Charles H. Wick

发明人： Charles H. Wick

IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC分类号： G01N1/2202 ,  G01N15/065 ,  G01N2001/2223 ,  G01N2015/0046 ,  Y10T436/11

摘要： An improved system for collecting, detecting, and classifying submicron-sized particles in a sample comprising an Integrated Virus Detection System (IVDS) of the type wherein the detecting means includes a differential mobility analyzer (DMA) and condensation particle counter (CPC), the improvement comprising positioning an aerosol collector or an electrostatic collector between the differential mobility analyzer and the condensation particle counter wherein submicron-sized particles from the environmental sample are collected for further analysis.

摘要翻译： 一种用于在样品中收集，检测和分类亚微米尺寸颗粒的改进系统，其包括其中检测装置包括差示迁移率分析仪（DMA）和冷凝颗粒计数器（CPC）的类型的综合病毒检测系统（IVDS）， 改进包括将微粒聚集体或静电收集器定位在差示迁移率分析仪和缩合颗粒计数器之间，其中收集来自环境样品的亚微米尺寸的颗粒用于进一步分析。

公开(公告)号：US07850908B1

公开(公告)日：2010-12-14

申请号：US11829357

申请日：2007-07-27

申请人： Charles H. Wick

发明人： Charles H. Wick

IPC分类号： C12Q1/68 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N9/30

CPC分类号： G01N15/065 ,  G01N1/2202 ,  G01N1/2273 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/1456 ,  G01N33/48714 ,  G01N33/497 ,  G01N2001/2223 ,  G01N2001/4066 ,  G01N2015/1486 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

摘要： A system and method for detecting the presence of submicron sized particles in a sample taken from the environment. More particularly, the system may be used to detect and identify bacteria by detecting the presence of bacterial pili which have been separated from bacterial cells in the sample. The system includes means for collecting a sample from the environment, separating pili from bacteria in the sample, and purifying and concentrating the submicron sized pili in the sample based on the size of the pili. The purified and concentrated pili are detected with an apparatus which includes an electrospray assembly having an electrospray capillary, a differential mobility analyzer which receives output from the capillary, and a condensation particle counting device for counting the number of pili sized particles that pass through the differential mobility analyzer.

摘要翻译： 用于检测取自环境的样品中亚微米尺寸颗粒的存在的系统和方法。 更具体地，该系统可用于通过检测已经从样品中的细菌细胞分离的细菌毛利的存在来检测和鉴定细菌。 该系统包括用于从环境中收集样品的装置，将样品中的细菌分离出来，并且根据小便的大小净化和浓缩样品中的亚微米尺寸的小便。 使用包括具有电喷雾毛细管的电喷雾组件，接收来自毛细管的输出的差分迁移率分析仪和用于计数通过差速器的粗大粒径的颗粒的冷凝颗粒计数装置的装置来检测纯化和浓缩的皮毛 移动分析仪。

公开(公告)号：US08224581B1

公开(公告)日：2012-07-17

申请号：US12570038

申请日：2009-09-30

申请人： Charles H. Wick ,  Michael F. Stanford ,  Alan W. Zulich ,  Samir V. Deshpande ,  Rabih E. Jabbour ,  Jacek P. Dworzanski ,  Patrick E. McCubbin

发明人： Charles H. Wick ,  Michael F. Stanford ,  Alan W. Zulich ,  Samir V. Deshpande ,  Rabih E. Jabbour ,  Jacek P. Dworzanski ,  Patrick E. McCubbin

IPC分类号： G01N33/48 ,  G01N33/50

CPC分类号： G01N33/6848 ,  C12Q1/04 ,  G01N33/5005

摘要： A process is provided for identifying a cell type in a sample that includes identification of one or more peptide sequences in the sample. Each peptide sequence is assigned to a protein of known sequence. A matrix of assignments is generated for the presence or absence of each peptide in one or more cells. The matrix of assignments is rearranged according to cell classification. A cell type based on the most probable cell classification is identified.

摘要翻译： 提供了用于鉴定样品中包含样品中一个或多个肽序列的鉴定的细胞类型的方法。 将每个肽序列分配给已知序列的蛋白质。 生成一个或多个细胞中各肽存在或不存在的分配矩阵。 分配矩阵根据细胞分类重新排列。 确定基于最可能的细胞分类的细胞类型。

公开(公告)号：US08021884B1

公开(公告)日：2011-09-20

申请号：US12964032

申请日：2010-12-09

申请人： Charles H. Wick

发明人： Charles H. Wick

IPC分类号： C12Q1/68 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N9/30

CPC分类号： G01N15/065 ,  G01N1/2202 ,  G01N1/2273 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/1456 ,  G01N33/48714 ,  G01N33/497 ,  G01N2001/2223 ,  G01N2001/4066 ,  G01N2015/1486 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/25875

摘要： A system and method for detecting the presence of submicron sized particles in a sample taken from the environment. More particularly, the system may be used to detect and identify bacteria by detecting the presence of bacterial pili which have been separated from bacterial cells in the sample. The system includes means for collecting a sample from the environment, separating pili from bacteria in the sample, and purifying and concentrating the submicron sized pili in the sample based on the size of the pili. The purified and concentrated pili are detected with an apparatus which includes an electrospray assembly having an electrospray capillary, a differential mobility analyzer which receives output from the capillary, and a condensation particle counting device for counting the number of pili sized particles that pass through the differential mobility analyzer.

摘要翻译： 用于检测取自环境的样品中亚微米尺寸颗粒的存在的系统和方法。 更具体地，该系统可用于通过检测已经从样品中的细菌细胞分离的细菌毛利的存在来检测和鉴定细菌。 该系统包括用于从环境中收集样品的装置，将样品中的细菌分离出来，并且根据小便的大小净化和浓缩样品中的亚微米尺寸的小便。 使用包括具有电喷雾毛细管的电喷雾组件，接收来自毛细管的输出的差分迁移率分析仪和用于计数通过差速器的粗大粒径的颗粒的冷凝颗粒计数装置的装置来检测纯化和浓缩的皮毛 移动分析仪。

公开(公告)号：US06238866B1

公开(公告)日：2001-05-29

申请号：US09246277

申请日：1999-02-08

申请人： Homer R. Yeh ,  Charles H. Wick

发明人： Homer R. Yeh ,  Charles H. Wick

IPC分类号： C12Q168

CPC分类号： C12Q1/6837 ,  C12Q2565/549 ,  C12Q2525/179

摘要： The present invention provides devices and methods for detecting or characterizing a nucleic acid analyte without requiring electrophoresis or the direct sequencing of analyte samples or analyte fragments. The device includes a panel or array of double stranded oligonucleotide probes immobilized on a solid support. each probe comprising a nucleotide sequence having a hypervariable number of tandem repeat sequences. Desirably, the specificity of the probes is varied with the location on the panel or array. One strand of each probe is preferably anchored at one terminus to a solid support and the opposite terminus of a second strand is not so anchored. The probes and/or the analyte are labeled by one or more reporter moieties, designed, for example, to allow for visual or instrument based detection of hybridization events.

摘要翻译： 本发明提供用于检测或表征核酸分析物而不需要电泳或分析物样品或分析物片段的直接测序的装置和方法。 该装置包括固定在固体支持物上的面板或双链寡核苷酸探针阵列。 每个探针包含具有高变数数目的串联重复序列的核苷酸序列。 期望地，探针的特异性随着面板或阵列上的位置而变化。 每个探针的一条链优选在一个末端锚定到固体支持物，而第二条链的相对末端不是如此锚定的。 探针和/或分析物被一个或多个报道分子标记，例如设计用于允许基于视觉或仪器的杂交事件的检测。