公开(公告)号：US11767499B2

公开(公告)日：2023-09-26

申请号：US16628810

申请日：2018-07-13

Applicant: CORNING INCORPORATED

Inventor： Gregory Roger Martin ,  Ana Maria Del Pilar Pardo ,  Michael Kurt Schaefer ,  Allison Jean Tanner

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/24 ,  C12M1/04 ,  C12M1/00

CPC classification number: C12M23/16 ,  B01L3/502715 ,  C12M23/08 ,  C12M23/24 ,  C12M29/04 ,  B01L2300/0681 ,  B01L2300/12

Abstract: A cell culture vessel (100) includes a wall including an inner surface defining a cell culture chamber of the vessel. A substrate (215) of non-porous material is positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber. The substrate includes a plurality of microcavities (220), each microcavity of the plurality of microcavities includes a concave surface defining a well and an opening, the concave surface of each microcavity includes at least one aperture including a dimension less than or equal to about 15 microns defining a path from the well to the second region. A cell culture vessel including a substrate including a plurality of microcavities and a layer of porous material is also provided. Methods of culturing cells in the cell culture vessel are also provided.