公开(公告)号：US12209268B2

公开(公告)日：2025-01-28

申请号：US17334953

申请日：2021-05-31

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Raymond W. Bourdeau ,  Anupama Lakshmanan ,  Arash Farhadi ,  Mikhail G. Shapiro ,  Audrey Lee-Gosselin

IPC: C12N15/75 ,  A61K49/18 ,  A61K49/22 ,  C07K14/195 ,  C07K14/32 ,  C12N15/70 ,  C12N15/74 ,  C12Q1/10

Abstract: Hybrid gas vesicle gene cluster (GVGC) configured for expression in a prokaryotic host are described comprising gas vesicle assembly (GVA) genes native to a GVA prokaryotic species and capable of being expressed in a functional form in the prokaryotic host, and one or more gas vesicle structural (GVS) genes native to one or more GVS prokaryotic species, at least one of the one or more GVS prokaryotic species different from the GVA prokaryotic species, and related gas vesicle reporting (GVR) genetic circuits, genetic, vectors, engineered cells, and related compositions methods and systems to produce GVs, hybrid GVGC and/or image a target site.

公开(公告)号：US10975420B2

公开(公告)日：2021-04-13

申请号：US15384254

申请日：2016-12-19

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mikhail G. Shapiro ,  Dan I. Piraner ,  Mohamad H. Abedi ,  Brittany Moser ,  Audrey Lee-Gosselin

IPC: C12Q1/68 ,  C12Q1/6825 ,  B01L7/00 ,  C12N15/63 ,  C12N15/10 ,  C12N15/82 ,  G01N33/53 ,  C12N15/00

Abstract: Temperature sensitive transcriptional bioswitches and related genetic circuits and in particular bandpass and/or multiplex genetic circuits, vectors, cells, compositions methods and systems are described.

公开(公告)号：US11959127B2

公开(公告)日：2024-04-16

申请号：US17204782

申请日：2021-03-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Mikhail G. Shapiro ,  Dan Piraner ,  Mohamad H. Abedi ,  Brittany Moser ,  Audrey Lee-Gosselin

IPC: C12Q1/68 ,  B01L7/00 ,  C12N15/10 ,  C12N15/63 ,  C12N15/82 ,  C12Q1/6825 ,  G01N33/53 ,  C12N15/00

CPC classification number: C12Q1/6825 ,  B01L7/52 ,  C12N15/1055 ,  C12N15/63 ,  C12N15/8217 ,  G01N33/53 ,  C12N15/00 ,  C12Q2525/30

Abstract: Temperature sensitive transcriptional bioswitches and related genetic circuits and in particular bandpass and/or multiplex genetic circuits, vectors, cells, compositions methods and systems are described.

公开(公告)号：US11118210B2

公开(公告)日：2021-09-14

申请号：US15663635

申请日：2017-07-28

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Raymond W. Bourdeau ,  Anupama Lakshmanan ,  Arash Farhadi ,  Mikhail G. Shapiro ,  Audrey Lee-Gosselin

IPC: C12Q1/10 ,  A61K49/22 ,  A61K49/18 ,  C12N15/74 ,  C07K14/32 ,  C12N15/70 ,  C07K14/195

Abstract: Hybrid gas vesicle gene cluster (GVGC) configured for expression in a prokaryotic host are described comprising gas vesicle assembly (GVA) genes native to a GVA prokaryotic species and capable of being expressed in a functional form in the prokaryotic host, and one or more gas vesicle structural (GVS) genes native to one or more GVS prokaryotic species, at least one of the one or more GVS prokaryotic species different from the GVA prokaryotic species, and related gas vesicle reporting (GVR) genetic circuits, genetic, vectors, engineered cells, and related compositions methods and systems to produce GVs, hybrid GVGC and/or image a target site.