公开(公告)号：US12275989B2

公开(公告)日：2025-04-15

申请号：US18386975

申请日：2023-11-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yi Xue ,  Joshua Ritchey ,  Robert Meltzer

IPC: C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6816

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

公开(公告)号：US11759780B2

公开(公告)日：2023-09-19

申请号：US17153311

申请日：2021-01-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Joshua Ritchey ,  Yi Xue ,  Robert Meltzer

IPC: B01L3/00 ,  C09D183/04 ,  C09D183/00 ,  B81C1/00 ,  C12Q1/686

CPC classification number: B01L3/50273 ,  B01L3/502707 ,  B01L3/502784 ,  B81C1/00206 ,  C09D183/00 ,  C09D183/04 ,  B01L2200/06 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2400/04 ,  C12Q1/686 ,  C09D183/04 ,  C08K3/36

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

公开(公告)号：US11857968B2

公开(公告)日：2024-01-02

申请号：US17357860

申请日：2021-06-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lung-Hsin Hung ,  Robert Meltzer ,  Lucas Frenz

IPC: B01L3/00 ,  C12Q1/6869

CPC classification number: B01L3/502761 ,  B01L3/502784 ,  C12Q1/6869 ,  B01L3/502776 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/143 ,  B01L2400/0487 ,  Y10T436/2575

Abstract: Microfluidic methods of altering the spacing of a stream of objects. In an exemplary method, objects of the object stream may be transported in carrier fluid along a microfluidic channel structure having an inflow region, an outflow region, and an expanded region extending from the inflow region to the outflow region. The expanded region may have a greater cross-sectional area for fluid flow than each of the inflow region and the outflow region. Objects of the object stream may be moved from the inflow region to the expanded region such that at least a subset of such objects are moved closer to one another. Objects of the object stream may be passed from the expanded region to the outflow region to increase a distance between such objects.

公开(公告)号：US10919038B2

公开(公告)日：2021-02-16

申请号：US15855913

申请日：2017-12-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Joshua Ritchey ,  Yi Xue ,  Robert Meltzer

IPC: C09D183/04 ,  B01L3/00 ,  C09D183/00 ,  B81C1/00 ,  C12Q1/686

Abstract: Compositions, devices, and methods are disclosed for the modification of polymer surfaces with coatings having a dispersion of silicone polymer and hydrophobic silica. The surface coatings provide the polymer surface with high hydrophobicity, as well as increased resistance to biofouling with proteinaceous material. The polymer surfaces can be particularly useful in microfluidic devices and methods that involve the contacting of the covalently modified polymer surfaces with emulsions of aqueous droplets containing biological macromolecules within an oil carrier phase.

公开(公告)号：US11845980B2

公开(公告)日：2023-12-19

申请号：US17665170

申请日：2022-02-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yi Xue ,  Joshua Ritchey ,  Robert Meltzer

IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6844 ,  C12Q1/6816

CPC classification number: C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6846 ,  C12Q1/6816 ,  C12Q2527/125 ,  C12Q2563/159 ,  C12Q1/6846 ,  C12Q2527/137 ,  C12Q2563/159

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

公开(公告)号：US11274337B2

公开(公告)日：2022-03-15

申请号：US16921453

申请日：2020-07-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yi Xue ,  Joshua Ritchey ,  Robert Meltzer

IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6844 ,  C12Q1/6816

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

公开(公告)号：US20210316309A1

公开(公告)日：2021-10-14

申请号：US17357860

申请日：2021-06-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lung-Hsin Hung ,  Robert Meltzer ,  Lucas Frenz

IPC: B01L3/00 ,  C12Q1/6869

Abstract: Microfluidic methods of altering the spacing of a stream of objects. In an exemplary method, objects of the object stream may be transported in carrier fluid along a microfluidic channel structure having an inflow region, an outflow region, and an expanded region extending from the inflow region to the outflow region. The expanded region may have a greater cross-sectional area for fluid flow than each of the inflow region and the outflow region. Objects of the object stream may be moved from the inflow region to the expanded region such that at least a subset of such objects are moved closer to one another. Objects of the object stream may be passed from the expanded region to the outflow region to increase a distance between such objects.

公开(公告)号：US11118217B2

公开(公告)日：2021-09-14

申请号：US15882098

申请日：2018-01-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yi Xue ,  Joshua Ritchey ,  Robert Meltzer

IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6844 ,  C12Q1/6816

Abstract: Emulsion compositions are provided herein. Also provided herein are kits containing one or more emulsion compositions or components for making such emulsion compositions. Also provided herein are methods of using such emulsion compositions, such as for amplification of target nucleic acids in emulsion droplets.

公开(公告)号：US11045805B2

公开(公告)日：2021-06-29

申请号：US16178300

申请日：2018-11-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lung-Hsin Hung ,  Robert Meltzer ,  Lucas Frenz

IPC: B01L3/00 ,  C12Q1/6869

Abstract: Microfluidic systems and methods for arranging a set of objects. In an exemplary method, the set of objects may be transported in carrier fluid along a microfluidic channel structure having a reformatting zone including an object-accessible region and at least one object-excluding region. A portion of the carrier fluid may be moved from the object-accessible region to the at least one object-excluding region in an upstream section of the reformatting zone, to reduce a spacing of objects of the set. The portion of the carrier fluid may be directed into the object-accessible region from the at least one object-excluding region in a downstream section of the reformatting zone, to increase a spacing of objects of the set. The steps of moving and directing in combination may increase the spacing between objects disproportionately for a subset of the objects that are closest to one another.