公开(公告)号：US20230001417A1

公开(公告)日：2023-01-05

申请号：US17837401

申请日：2022-06-10

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Ammon David Lentz ,  Kevin Bailey ,  Dustin Diemert ,  Joel Daniel Krayer ,  Laurence Michael Vaughan

IPC: B01L3/00 ,  B65D51/00

Abstract: A pierceable cap 11 may be used for containing sample specimens. The pierceable cap 11 may prevent escape of sample specimens before transfer with a transfer device 43. The pierceable cap 11 may fit over a vessel 21. An access port in the shell of the pierceable cap 11 may allow passage of a transfer device 43 through the pierceable cap 11. At least one frangible layer 215, 216 may be configured with cross slits 506 in a particular cross slit geometry. The cross slits 506 may contain an openable portion 644 or be covered by a thin membrane 645. The shell 610 and frangible layer(s) 215, 216 may be integrated into a one piece cap 601, or be separate components 634. The membrane on which the cross slits 506 are placed can be flat or contoured to guide the transfer device 43 to the cross slits 506.

公开(公告)号：US20200094256A1

公开(公告)日：2020-03-26

申请号：US15372021

申请日：2016-12-07

Applicant: Becton, Dickinson and Company

Inventor： Ammon David Lentz ,  Kevin Bailey ,  Dustin Diemert ,  Joel Daniel Krayer ,  Laurence Michael Vaughan

IPC: B01L3/00 ,  B65D51/00

Abstract: A pierceable cap 11 may be used for containing sample specimens. The pierceable cap 11 may prevent escape of sample specimens before transfer with a transfer device 43. The pierceable cap 11 may fit over a vessel 21. An access port in the shell of the pierceable cap 11 may allow passage of a transfer device 43 through the pierceable cap 11. At least one frangible layer 215, 216 may be configured with cross slits 506 in a particular cross slit geometry. The cross slits 506 may contain an openable portion 644 or be covered by a thin membrane 645. The shell 610 and frangible layer(s) 215, 216 may be integrated into a one piece cap 601, or be separate components 634. The membrane on which the cross slits 506 are placed can be flat or contoured to guide the transfer device 43 to the cross slits 506.

公开(公告)号：US20220372554A1

公开(公告)日：2022-11-24

申请号：US17883403

申请日：2022-08-08

Applicant: Becton, Dickinson and Company

Inventor： Feng Yang ,  Sha-Sha Wang ,  Laurence Michael Vaughan ,  Michael Porter ,  Elaine Rose

IPC: C12Q1/6806 ,  C12N1/06 ,  C12Q1/70 ,  G01N33/569

Abstract: A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

公开(公告)号：US11434519B2

公开(公告)日：2022-09-06

申请号：US16399305

申请日：2019-04-30

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Feng Yang ,  Sha-Sha Wang ,  Laurence Michael Vaughan ,  Michael Porter ,  Elaine Rose

IPC: C12Q1/68 ,  C12N1/06 ,  C12Q1/70 ,  C12Q1/6806 ,  G01N33/569

Abstract: A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

公开(公告)号：US20190256893A1

公开(公告)日：2019-08-22

申请号：US16399305

申请日：2019-04-30

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Feng Yang ,  Sha-Sha Wang ,  Laurence Michael Vaughan ,  Michael Porter ,  Elaine Rose

IPC: C12Q1/6806 ,  G01N33/569 ,  C12N1/06 ,  C12Q1/70

Abstract: A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

公开(公告)号：US10323267B2

公开(公告)日：2019-06-18

申请号：US15067428

申请日：2016-03-11

Applicant: Becton, Dickinson and Company

Inventor： Feng Yang ,  Sha-Sha Wang ,  Laurence Michael Vaughan ,  Michael Porter ,  Elaine Rose

IPC: C12Q1/68 ,  C12N1/06 ,  C12Q1/70 ,  C12Q1/6806 ,  G01N33/569

Abstract: A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

公开(公告)号：US20160194687A1

公开(公告)日：2016-07-07

申请号：US15067428

申请日：2016-03-11

Applicant: Becton, Dickinson and Company

Inventor： Feng Yang ,  Sha-Sha Wang ,  Laurence Michael Vaughan ,  Michael Porter ,  Elaine Rose

IPC: C12Q1/68 ,  G01N33/569 ,  C12Q1/70 ,  C12N1/06

CPC classification number: C12Q1/6806 ,  C12N1/06 ,  C12Q1/708 ,  G01N33/56983 ,  G01N2333/025 ,  C12Q2527/137 ,  C12Q2527/125

Abstract: A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

Abstract translation: 直接化学裂解组合物包括测定相容缓冲液组合物和测定相容性表面活性剂。 当与样品储存组合物组合时，这种组合物防止对生物样品中细胞裂解的核酸和蛋白质的不期望的修饰。 来自这些组合物的样品的测定不需要昂贵且耗时的步骤，例如离心和长时间的高温处理。 本发明的直接化学溶解组合物允许从生物样品中的细胞中直接进行核酸提取，而不需要从运输介质中滗出或以其它方式与测定相容的缓冲液交换传输介质。 不需要将样品与蛋白酶K或其他酶组合从细胞中提取核酸。 还考虑了用于裂解细胞以获得用于测定的靶核酸的方法和用于将直接化学裂解组合物与样品组合的试剂盒。